D66 :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	525,978	T→G	S64A (TCT→GCT)	SAUSA300_RS02510 →	methionine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	525,978	0	T	G	99.5%	534.9 / ‑4.9	198	S64A (TCT→GCT)	SAUSA300_RS02510	methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (196/1); total (197/1)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.42e-01

Only 100 of 199 total aligned reads displayed.

TATTGCAAGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAATCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCAGAACA  >  NC_007793/525884‑526076

tATTGCAAGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGt                                                                                               >  1:2109360/1‑100 (MQ=255)

   tGCAAGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAg                                                                                            >  1:180835/1‑100 (MQ=255)

    tcAAGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGa                                                                                           >  1:1514939/2‑100 (MQ=255)

     cAAGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGAc                                                                                          >  1:5382507/1‑100 (MQ=255)

       aGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAg                                                                                        >  1:4668636/1‑100 (MQ=255)

        gATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGa                                                                                       >  1:163518/1‑100 (MQ=255)

          gattaagtaaTGCAAGGATATGATGTTCGCTATTTGACTTGAACGGATGAACACGGTCAAAAAATTCACGAAAAAGCTCAAAACGCTGGTAAGACAGaat                                                                                     >  1:642606/9‑99 (MQ=255)

           ataAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAAt                                                                                    >  1:5047158/1‑100 (MQ=255)

           ataAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAAt                                                                                    >  1:1381738/1‑100 (MQ=255)

             aaGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTg                                                                                  >  1:1609349/1‑100 (MQ=255)

             aaGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATCCAAGAAAAAGCTCAAAAAGCTGGTAAGatcggaagag                                                                                  >  1:5143370/1‑91 (MQ=255)

               tagaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGaa                                                                                >  1:3650055/2‑100 (MQ=255)

               ctgaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGATATTGaa                                                                                >  1:4601330/3‑100 (MQ=255)

               aagaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTcagacagatcggaag                                                                                >  1:2433058/2‑93 (MQ=255)

                tgaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAat                                                                               >  1:3806056/2‑100 (MQ=255)

                tgaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAat                                                                               >  1:3590955/2‑100 (MQ=255)

                ctaaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAat                                                                               >  1:2555336/3‑100 (MQ=255)

                atctcgCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAat                                                                               >  1:2802725/6‑100 (MQ=255)

                agaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAat                                                                               >  1:5746510/1‑100 (MQ=255)

                agaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAat                                                                               >  1:5284423/1‑100 (MQ=255)

                agaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAat                                                                               >  1:1907014/1‑100 (MQ=255)

                agaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAat                                                                               >  1:1843532/1‑100 (MQ=255)

                 taaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAata                                                                              >  1:3397496/2‑100 (MQ=255)

                 taaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAata                                                                              >  1:4276485/2‑100 (MQ=255)

                 gaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTTAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAata                                                                              >  1:4779950/1‑100 (MQ=255)

                 gaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAata                                                                              >  1:2003316/1‑100 (MQ=255)

                 gaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAata                                                                              >  1:5452222/1‑100 (MQ=255)

                 gaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAata                                                                              >  1:1588102/1‑100 (MQ=255)

                 gaATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAata                                                                              >  1:1664110/1‑100 (MQ=255)

                 caaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACCGAAATTGAata                                                                              >  1:2993028/2‑100 (MQ=255)

                  taTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAatat                                                                             >  1:2742360/2‑100 (MQ=255)

                  aaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTTAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAatat                                                                             >  1:1238402/1‑100 (MQ=255)

                  aaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAatat                                                                             >  1:3260151/1‑100 (MQ=255)

                  aaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAatat                                                                             >  1:2605406/1‑100 (MQ=255)

                  aaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAatat                                                                             >  1:3918408/1‑100 (MQ=255)

                  aaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAatat                                                                             >  1:2434186/1‑100 (MQ=255)

                  aaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAatat                                                                             >  1:5241369/1‑100 (MQ=255)

                  aaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAatat                                                                             >  1:5503052/1‑100 (MQ=255)

                  aaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAatat                                                                             >  1:1016297/1‑100 (MQ=255)

                  aaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAatat                                                                             >  1:1205685/1‑100 (MQ=255)

                  aaTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAatat                                                                             >  1:1279948/1‑100 (MQ=255)

                   ctGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATAtt                                                                            >  1:101659/2‑100 (MQ=255)

                   ctGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATAtt                                                                            >  1:5534258/2‑100 (MQ=255)

                   aTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATAtt                                                                            >  1:4984929/1‑100 (MQ=255)

                   aTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATAtt                                                                            >  1:3691030/1‑100 (MQ=255)

                   aTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATAtt                                                                            >  1:2503766/1‑100 (MQ=255)

                   aTGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATAtt                                                                            >  1:5784126/1‑100 (MQ=255)

                    tGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATAttt                                                                           >  1:4051300/1‑100 (MQ=255)

                    tGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATAtta                                                                           >  1:3851232/1‑99 (MQ=255)

                     gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATAttgg                                                                          >  1:1708885/1‑98 (MQ=255)

                     gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATAttcg                                                                          >  1:4364709/1‑98 (MQ=255)

                     gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATAttcg                                                                          >  1:3760106/1‑98 (MQ=255)

                     gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATAttag                                                                          >  1:2159747/1‑98 (MQ=255)

                     gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTg                                                                          >  1:1439406/1‑100 (MQ=255)

                     gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTg                                                                          >  1:571720/1‑100 (MQ=255)

                     gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTg                                                                          >  1:3057067/1‑100 (MQ=255)

                     gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTg                                                                          >  1:4093533/1‑100 (MQ=255)

                     gCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTg                                                                          >  1:273779/1‑100 (MQ=255)

                     ccAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATAttgg                                                                          >  1:1763895/2‑98 (MQ=255)

                       caGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGga                                                                        >  1:28760/2‑100 (MQ=255)

                       aaGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGga                                                                        >  1:3281431/1‑100 (MQ=255)

                       aaGGATATGATGTTCGCTATCTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAGGCTGGTAAGACAGAAATTGAATATTCGga                                                                        >  1:772618/1‑100 (MQ=255)

                             atgatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGgatgagat                                                                  >  1:4459722/1‑100 (MQ=255)

                             atgatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGaatgagat                                                                  >  1:46171/1‑100 (MQ=255)

                             atgatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTCGgatgagat                                                                  >  1:2175283/1‑100 (MQ=255)

                              agatgTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGgatgagatc                                                                 >  1:1492278/2‑99 (MQ=255)

                               gatgTTCGCTATTTGACTGGAGCGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTCGgatgagatcg                                                                >  1:2509072/1‑98 (MQ=255)

                                  ccacGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTCGgatgagatcggaa                                                             >  1:3852867/4‑95 (MQ=255)

                                     cGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGgatgagatcggaagag                                                          >  1:3309853/1‑92 (MQ=255)

                                     cGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTCGgatgagatcggaagag                                                          >  1:5261594/1‑92 (MQ=255)

                                     cGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTAGgatgagatcggaagag                                                          >  1:5388481/1‑92 (MQ=255)

                                         aTTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGaga                                                      >  1:4729129/1‑98 (MQ=255)

                                         aTTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAAt                                                      >  1:1848596/1‑100 (MQ=255)

                                             gACTGGAACGGATGAACACGGTC‑AAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAAc                                                 >  1:3354512/1‑100 (MQ=255)

                                              attGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAAc                                                 >  1:5183336/3‑100 (MQ=255)

                                              aCTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAAc                                                 >  1:299827/1‑100 (MQ=255)

                                               cTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACa                                                >  1:5086147/1‑100 (MQ=255)

                                                 ggAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAAt                                              >  1:721662/1‑100 (MQ=255)

                                                 ggAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAAt                                              >  1:4000555/1‑100 (MQ=255)

                                                 ggAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAAt                                              >  1:564479/1‑100 (MQ=255)

                                                 ggAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAAt                                              >  1:2273014/1‑100 (MQ=255)

                                                    ccGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATtgt                                           >  1:2055519/2‑100 (MQ=255)

                                                       tgtGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTggg                                        >  1:38135/3‑100 (MQ=255)

                                                       ctggAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTggg                                        >  1:1075911/4‑100 (MQ=255)

                                                        aTGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGc                                       >  1:599273/1‑100 (MQ=255)

                                                          gcacacGGTC‑AAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTaa                                    >  1:4473528/3‑100 (MQ=255)

                                                          gAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTa                                     >  1:1139344/1‑100 (MQ=255)

                                                          aaaCACGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTa                                     >  1:2511422/2‑100 (MQ=255)

                                                           gacacGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTaa                                    >  1:3662274/2‑100 (MQ=255)

                                                            acacGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAg                                   >  1:2389518/1‑100 (MQ=255)

                                                            acacGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAg                                   >  1:4579091/1‑100 (MQ=255)

                                                            acacGGTCAAAAAATTCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTAGGCTAAg                                   >  1:5347802/1‑100 (MQ=255)

                                                                         cctCAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCTTGAAATTTCaa                      >  1:3566164/3‑100 (MQ=255)

                                                                            cAAGAAAAAGCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCTTGAAATTTCAAatg                   >  1:4267414/1‑100 (MQ=255)

                                                                                        cAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCa       >  1:2246948/1‑100 (MQ=255)

                                                                                        cAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCa       >  1:5038168/1‑100 (MQ=255)

                                                                                        cAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCa       >  1:4681687/1‑100 (MQ=255)

                                                                                         aaaaaGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCAg      >  1:3631104/1‑100 (MQ=255)

                                                                                           aaaGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCAGaa    >  1:2316727/1‑100 (MQ=255)

                                                                                             ctCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCAGaaca  >  1:159516/2‑100 (MQ=255)

TATTGCAAGATATAAGAGAATGCAAGGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAAGCTCAAAAATCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAACAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCAGAACA  >  NC_007793/525884‑526076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`