Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	524,099	C→T	A135V (GCA→GTA)	SAUSA300_RS02490 →	O‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	524,099	0	C	T	100.0%	49.1 / NA	19	A135V (GCA→GTA)	SAUSA300_RS02490	O‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/5);  total (14/5)

GTTACTAAAGTATTTAAACCTTCACAATATACTTTAGTAACGTGTAATCCGCCTTATTTTAAAGAGAATCAGCAACACCAACATCAAAAAGAAGCACATAAGATAGCGAGACATGAGATTATGTGTACACTTGAAGATTGCATGATTGCAGCCCGTCATTTATTAAAAGAAGGTGGCAGGCTAAAC  >  NC_007793/524005‑524190                                                                                               |                                                                                            gTTACTAAAGTATTTAAACCTTCACAATATACTTTAGTAACGTGTAATCCGCCTTATTTTAAAGAGAATCAGCAACACCAACATCAAAAAGAAGTACATa                                                                                        <  1:1347640/100‑1 (MQ=255) gTTACTAAAGTATTTAAACCTTCACAATATACTTTAGTAACGTGTAATCCGCCTTATTTTAAAGAGAATCAGCAACACCAACATCAAAAAGAAGTACATa                                                                                        <  1:5594698/100‑1 (MQ=255)        aaGTATTTAAACCTTCACAATATACTTTAGTATCGTGTAATCCGCCTTATTTTAAAGAGAATCAGCAACACCAACATC‑AAAAGAAGTACATAAGATAGCg                                                                                <  1:1448279/100‑1 (MQ=255)                aaaCCTTCACAATATACTTTAGTAACGTGTAATCCGCCTTATTTTAAAGAGAATCAGCAACACCAACATCAAAAGGAAGTACATAAGATAGCGAGACATg                                                                         <  1:453133/100‑1 (MQ=255)                      tCACAATATACTTTAGTAACGTGTAATCCGCCTTATTTTAAAGAGAATCAGCAACACCAACATCAAAAAGAAGTACAAAAGATAGCGAGACATGAGAtcg                                                                   >  1:4026111/1‑98 (MQ=255)                                   tAGTAACGTGTAATCCGCCTTATTTTAAAGAGAATCAGCAACACCAACATCAAAAAGAAGTACATAAGATAGCGAGACATGAGATTATGTGTACACTTGa                                                      <  1:2594902/100‑1 (MQ=255)                                   tAGTAACGTGTAATCCGCCTTATTTTAAAGAGAATCAGCAACACCAACATCAAAAAGAAGTACATAAGATAGCGAGACATGAGATTATGTGTACACTTGa                                                      >  1:4187368/1‑100 (MQ=255)                                       taCGTGTAATCCGCCTTATTTTAAAGAGAATCAGCAACACCAACATCAAAAAGAAGTACATAAGATAGCGAGACATGAGATTATGTGTACACTTGAAGAt                                                  >  1:5651557/2‑100 (MQ=255)                                       aaCGTGTAATCCGCCTTATTTTAAAGAGAATCAGCAACACCAACATCAAAAAGAAGTACATAAGATAGCGAGACATGAGATTATGTGTACACTTGAAGAt                                                  >  1:907411/1‑100 (MQ=255)                                                 ccGCCTTATTTTAAAGAGAATCAGCAACACCAACATCAAAAAGAAGTACATAAGATAGCGAGACATGAGATTATGTGTACACTTGAAGATTGCATGATTg                                        >  1:5355715/1‑100 (MQ=255)                                                                      cAGCAACACCAACATCAAAAAGAAGTACATAAGATAGCGAGACATGAGATTATGTGTACACTTGAAGATTGCATGATTGCAGCCCGTCATTTATTAAaag                   >  1:5685415/1‑100 (MQ=255)                                                                      cAGCAACACCAACATCAAAAAGAAGTACATAAGATAGCGAGACATGAGATTATGTGTACACTTGAAGATTGCATGATTGCAGCCCGTCATTTATTAAaag                   >  1:579154/1‑100 (MQ=255)                                                                      cAGCAACACCAACATCAAAAAGAAGTACATAAGATAGCGAGACATGAGATTATGTGTACACTTGAAGATTGCATGATTGCAGCCCGTCATTTATTAAaag                   >  1:785183/1‑100 (MQ=255)                                                                       tgCAACACCAACATCAAAAAGAAGTACATAAGATAGCGAGACATGAGATTATGTGTACACTTGAAGATTGCATGATTGCAGCCCGTCATTTATTAAaaga                  >  1:1244659/2‑100 (MQ=255)                                                                        gCAACACCAACATCAAAAAGAAGTACATAAGATAGTGAGACATGAGATTATGTGTACACTTGAAGATTGCATGATTGCAGCCCGTCATTTATTAAaagaa                 >  1:1794648/1‑100 (MQ=255)                                                                        gCAACACCAACATCAAAAAGAAGTACATAAGATAGCGAGACATGAGATTATGTGTACACTTGAAGATTGCATGATTGCAGCCCGTCATTTATTAAaagaa                 >  1:3999954/1‑100 (MQ=255)                                                                           acacCAACATCAAAAAGAAGTACATAAGATAGCGAGACATGAGATTATGTGTACACTTGAAGATTGCATGATTGCAGCCCGTCATTTATTAAAAGAAGGt              >  1:4920019/1‑100 (MQ=255)                                                                              atcaCATCAAAAAGAAGTACATAAGATAGCGAGACATGAGATTATGTGTACACTTGAAGATTGCATGATTGCAGCCCGTCATTTATTAAAAGAAGGTGGc           >  1:4356327/4‑100 (MQ=255)                                                                                       taaaGAAGTACATAAGATAGCGAGACATGAGATTATGTGTACACTTGAAGATTGCATGATTGCAGCCCGTCATTTATTAAAAGAAGGTGGCAGGCTAAAc  >  1:4501295/2‑100 (MQ=255)                                                                                               |                                                                                            GTTACTAAAGTATTTAAACCTTCACAATATACTTTAGTAACGTGTAATCCGCCTTATTTTAAAGAGAATCAGCAACACCAACATCAAAAAGAAGCACATAAGATAGCGAGACATGAGATTATGTGTACACTTGAAGATTGCATGATTGCAGCCCGTCATTTATTAAAAGAAGGTGGCAGGCTAAAC  >  NC_007793/524005‑524190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑