Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	519,306	T→C	F169S (TTT→TCT)	SAUSA300_RS02455 →	lysine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	519,306	0	T	C	100.0%	24.6 / NA	11	F169S (TTT→TCT)	SAUSA300_RS02455	lysine decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/1);  total (10/1)

TGAATAATGACGGTCACAAACTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATTTTTGCACCAATTAAATATTCCTGTACTCATTGACGAAGCACACGGCGCGCACTTTGGATTGCA  >  NC_007793/519222‑519369                                                                                     |                                                                tGAATAATGACGGTCACAAACTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTaaa                                                  >  1:1906000/1‑100 (MQ=255)      aatGACGGTCACAAACTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTc                                             >  1:4813631/1‑100 (MQ=255)      aatGACGGTCACAAACTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTc                                             >  1:5729081/1‑100 (MQ=255)                 caAACTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCAtt                                  >  1:2246694/1‑100 (MQ=255)                    aCTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGAc                               >  1:4263374/1‑100 (MQ=255)                            atttGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGACGAAGcaca                       >  1:286643/3‑100 (MQ=255)                               tGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGACGAAGCACACgg                    >  1:5143910/1‑100 (MQ=255)                                   ctATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGACGAAGCACACGgcgcg                >  1:2415924/2‑100 (MQ=255)                                    tATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGACGAAGCACACGgcgcgc               >  1:3123803/1‑100 (MQ=255)                                          aaCTATTACGGTGAAACATTTAATGTAGACGAGGTTATCAAATCTTTGCACCAATTAAATATTCCTGTACTCATTGACGAAGCACACGGCGCGCACTTTg         >  1:5424284/1‑100 (MQ=255)                                                 ccGGTGAAACATTTAATGTAGAAGAGGTTATCAAATCTTTGCTCCAATTAAATATTCCTGTACTCATTGACGAAGCACACGGCGCGCACTTTGGATTGCa  <  1:5549406/99‑1 (MQ=255)                                                                                     |                                                                TGAATAATGACGGTCACAAACTTGCTGTGTTGACTTATCCTAACTATTACGGTGAAACATTTAATGTAGAAGAGGTTATCAAATTTTTGCACCAATTAAATATTCCTGTACTCATTGACGAAGCACACGGCGCGCACTTTGGATTGCA  >  NC_007793/519222‑519369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑