Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	509,629	G→C	G100G (GGG→GGC)	SAUSA300_RS02420 →	DNA polymerase III subunit gamma/tau

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	509,629	0	G	C	100.0%	222.3 / NA	83	G100G (GGG→GGC)	SAUSA300_RS02420	DNA polymerase III subunit gamma/tau
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (81/2);  total (81/2)

CTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTAAC  >  NC_007793/509532‑509724                                                                                                  |                                                                                                cTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGt                                                                                               >  1:887942/1‑100 (MQ=255)  tGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGtt                                                                                              >  1:3358272/1‑100 (MQ=255)   gctGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTtg                                                                                             >  1:3335644/3‑100 (MQ=255)   gATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTtg                                                                                             >  1:1154286/1‑100 (MQ=255)   gATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTtg                                                                                             >  1:4532926/1‑100 (MQ=255)   gATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTtg                                                                                             >  1:3915130/1‑100 (MQ=255)    aTGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTtga                                                                                            >  1:837966/1‑100 (MQ=255)      ggAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTtgatg                                                                                          >  1:5230599/1‑100 (MQ=255)      ggAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTtgatg                                                                                          >  1:3535593/1‑100 (MQ=255)       cagaACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTtgatga                                                                                         >  1:2365688/2‑100 (MQ=255)        agaACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGaa                                                                                        >  1:4362760/1‑100 (MQ=255)          aaCCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAAt                                                                                      >  1:2956840/1‑100 (MQ=255)               tGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAGTAAGag                                                                                 >  1:1878640/1‑99 (MQ=255)               tGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGag                                                                                 >  1:974119/1‑99 (MQ=255)               tGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGaa                                                                                 >  1:187409/1‑100 (MQ=255)                gTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGaaa                                                                                >  1:5076406/1‑100 (MQ=255)                      aatgTCATAGTTGTAAAGGCATTACGCAGGGGAATAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTag                                                                          <  1:5586145/100‑1 (MQ=255)                                   tctaGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTaaa                                                             >  1:1402195/4‑100 (MQ=255)                                    acaGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAat                                                            >  1:3110592/3‑100 (MQ=255)                                     caGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAGATATTAGAGACAAAGTTAAata                                                           >  1:1556976/2‑100 (MQ=255)                                      aGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAatat                                                          >  1:4732406/1‑100 (MQ=255)                                          ctgccGCAGGGGACTAATTCAGATGTGATATAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCAc                                                      >  1:4296194/5‑100 (MQ=255)                                          atgtggCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCAc                                                      >  1:2254844/6‑100 (MQ=255)                                          aTTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCAc                                                      >  1:1386742/1‑100 (MQ=255)                                          aTTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCAc                                                      >  1:5155420/1‑100 (MQ=255)                                           ttACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATACGCAcc                                                     >  1:882083/1‑100 (MQ=255)                                           atACGCAGGGGACTAATTCAGATGTGCTAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCAcc                                                     >  1:3798265/2‑100 (MQ=255)                                           agctgCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCAcc                                                     >  1:4277728/5‑100 (MQ=255)                                            tACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCa                                                    >  1:5193422/1‑100 (MQ=255)                                            tACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCa                                                    >  1:3022580/1‑100 (MQ=255)                                            tACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCa                                                    >  1:5650727/1‑100 (MQ=255)                                            tACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCa                                                    >  1:2532861/1‑100 (MQ=255)                                            tACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCa                                                    >  1:2463043/1‑100 (MQ=255)                                            tACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCa                                                    >  1:328829/1‑100 (MQ=255)                                            tACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCa                                                    >  1:426501/1‑100 (MQ=255)                                             aCGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCaa                                                   >  1:551565/1‑100 (MQ=255)                                             aCGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCaa                                                   >  1:5772867/1‑100 (MQ=255)                                             aCGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCaa                                                   >  1:1415810/1‑100 (MQ=255)                                             aCGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCaa                                                   >  1:4151710/1‑100 (MQ=255)                                             aCGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCaa                                                   >  1:1901304/1‑100 (MQ=255)                                             aCGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCaa                                                   >  1:2254812/1‑100 (MQ=255)                                             aCGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCaa                                                   >  1:3508140/1‑100 (MQ=255)                                             aCGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCaa                                                   >  1:2028673/1‑100 (MQ=255)                                              tccAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAg                                                  >  1:565356/3‑100 (MQ=255)                                                 ctgggACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGa                                               >  1:4817295/3‑100 (MQ=255)                                                    ggACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATc                                            >  1:5661277/1‑100 (MQ=255)                                                         aaTTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGagatcggaa                                       >  1:3562125/1‑92 (MQ=255)                                                         aaTTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAat                                       >  1:3328306/1‑100 (MQ=255)                                                             cAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATaa                                   >  1:1662830/1‑100 (MQ=255)                                                                  gtgATAGAAATTGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGttt                              >  1:3689947/1‑100 (MQ=255)                                                                            ttGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGa                    >  1:2766637/1‑100 (MQ=255)                                                                            ctGATGCTGCTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGa                    >  1:2455479/2‑100 (MQ=255)                                                                              cgatctgctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATg                  <  1:217673/96‑1 (MQ=255)                                                                               tggctgctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGa                 >  1:2196949/3‑100 (MQ=255)                                                                               ctgctgcTAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGa                 >  1:239001/2‑100 (MQ=255)                                                                                 cctgctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAgg               >  1:4253167/2‑100 (MQ=255)                                                                                  ctgctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGt              >  1:1913745/1‑100 (MQ=255)                                                                                  ctgctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGt              >  1:3303556/1‑100 (MQ=255)                                                                                  ctgctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGt              >  1:5123291/1‑100 (MQ=255)                                                                                   tgctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTg             >  1:4645043/1‑100 (MQ=255)                                                                                   tgctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTg             >  1:5239540/1‑100 (MQ=255)                                                                                    gctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAACGTTTATATTATAGATGAGGTGc            >  1:5076447/1‑100 (MQ=255)                                                                                    gctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGc            >  1:3269968/1‑100 (MQ=255)                                                                                    gctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGc            >  1:5121907/1‑100 (MQ=255)                                                                                    gctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGc            >  1:2453549/1‑100 (MQ=255)                                                                                    gctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGc            >  1:3600859/1‑100 (MQ=255)                                                                                    gctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGc            >  1:5475039/1‑100 (MQ=255)                                                                                    gctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGc            >  1:3124603/1‑100 (MQ=255)                                                                                    gctAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATACAAAGTTTATATTATAGATGAGGTGc            >  1:2705130/1‑100 (MQ=255)                                                                                     gtAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAGAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGca           >  1:2711858/2‑100 (MQ=255)                                                                                      tAGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGcac          >  1:3198156/1‑100 (MQ=255)                                                                                       ggTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGcaca         >  1:2739835/2‑100 (MQ=255)                                                                                       aGTAATAATGGCGTTGATGAAATAAGACATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGcaca         >  1:971551/1‑100 (MQ=255)                                                                                       aGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGtaca         >  1:2677761/1‑100 (MQ=255)                                                                                       aGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGcaca         >  1:563667/1‑100 (MQ=255)                                                                                       aGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGcaca         >  1:765344/1‑100 (MQ=255)                                                                                       aGTAATAATGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGcaca         >  1:2490662/1‑100 (MQ=255)                                                                                            taatGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTa    >  1:5490432/1‑100 (MQ=255)                                                                                             catGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTaa   >  1:604658/2‑100 (MQ=255)                                                                                             aatGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTaa   >  1:4013709/1‑100 (MQ=255)                                                                                             aatGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTaa   >  1:3270037/1‑100 (MQ=255)                                                                                              ctGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTaac  >  1:320488/2‑100 (MQ=255)                                                                                              atGGCGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTaac  >  1:3862546/1‑100 (MQ=255)                                                                                                  |                                                                                                CTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAAGGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGGGTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATATAAAGTTTATATTATAGATGAGGTGCACATGCTAAC  >  NC_007793/509532‑509724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑