Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	484,615	T→C	M1T (ATG→ACG) †	SAUSA300_RS02310 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	484,615	0	T	C	100.0%	107.4 / NA	41	M1T (ATG→ACG) †	SAUSA300_RS02310	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/10);  total (31/10)

GGGAACTTAGCTAAAGTTAATTTGTAGTGTGATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTATGGGCATTGCACCTGAAGTTGTTATGTGGCGAGATGTTGGGGATATAGATGGTGTTGTATGTGG‑CGAGATATTGGCGCAC  >  NC_007793/484518‑484694                                                                                                  |                                                                                 tggAACTTAGCTAAAGTTAATTTGTAGTGTGATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACgg                                                                                >  1:1298070/2‑100 (MQ=255)    aaCTTAGCTAAAGTTAATTTGTAGTGTGATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCa                                                                             <  1:59746/100‑1 (MQ=255)        tAGCTAAAGTTAATTTGTAGTGTGATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGc                                                                         >  1:3053322/1‑100 (MQ=255)        aaGCTAAAGTTAATTTGTAGTGTGATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGc                                                                         >  1:2783031/2‑100 (MQ=255)             aaaGTTAATTTGTAGTGTGATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTg                                                                    >  1:819557/1‑100 (MQ=255)                     tttGTAGTGTGATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAgttgtt                                                            <  1:2501152/100‑1 (MQ=255)                      ttGTAGTGTGATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTa                                                           >  1:3504508/1‑100 (MQ=255)                      ttGTAGTGTGATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTa                                                           >  1:4122450/1‑100 (MQ=255)                      ttGTAGTGTGATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTa                                                           >  1:2783324/1‑100 (MQ=255)                      ttGTAGTGTGATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTa                                                           >  1:180049/1‑100 (MQ=255)                      aggTAGTGTGATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTa                                                           >  1:1123300/3‑100 (MQ=255)                            tgtgATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGc                                                     >  1:4359205/1‑100 (MQ=255)                            tgtgATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGc                                                     >  1:2826875/1‑100 (MQ=255)                             gtgATGTCGTGGTAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATCTGGCg                                                    >  1:1066454/1‑100 (MQ=255)                             gtgATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATgcggcg                                                    >  1:5581930/1‑100 (MQ=255)                             gtgATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCg                                                    >  1:5054846/1‑100 (MQ=255)                             gtgATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCg                                                    <  1:3676363/100‑1 (MQ=255)                             gtgATGTCGTGGAAATATTATGGAGCTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGca                                                    >  1:1787411/1‑99 (MQ=255)                             cgatcttCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCg                                                    <  1:2099614/94‑1 (MQ=255)                              gcttGTCGTGGAAATATTATGGAGTTATACCAGAAGCTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCga                                                   >  1:3499106/4‑100 (MQ=255)                               gATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCgag                                                  >  1:1710049/1‑100 (MQ=255)                               gATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCgag                                                  <  1:4995298/100‑1 (MQ=255)                               gATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGGGGCgag                                                  >  1:2039820/1‑100 (MQ=255)                               gATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATATGGCgag                                                  >  1:3901519/1‑100 (MQ=255)                                  gTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGCCGAGAtc                                               >  1:2517567/1‑99 (MQ=255)                                  gTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGCGGCGAGAtc                                               >  1:4801830/1‑99 (MQ=255)                                    tctGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTAGCGAGAtcgg                                             >  1:5104957/3‑97 (MQ=255)                                    cGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCGAGATGtt                                             >  1:300666/1‑100 (MQ=255)                                    cGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCGAGATGtt                                             >  1:370904/1‑100 (MQ=255)                                    cGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCGAGATGtt                                             >  1:5753044/1‑100 (MQ=255)                                     gTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATG‑AGCGAGAtcggaa                                           >  1:21911/1‑95 (MQ=255)                                       ggAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCGAGATGTTggg                                          >  1:5733614/1‑100 (MQ=255)                                        gatctattgtGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCGAGATGTTgggc                                         <  1:4527210/96‑2 (MQ=255)                                            tattatGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCGAGATGTTGGGGatat                                     <  1:4676354/100‑1 (MQ=255)                                            tattatGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCGAGATGTTGGGGatat                                     <  1:1345395/100‑1 (MQ=255)                                                                 gTTGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCGAGATGTTGGGGATATAGATGGTGTTGTATGTGG‑ctt               <  1:3725373/100‑3 (MQ=255)                                                                   tGATTTGTATTGTAATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCGAGATGTTGGGGATATAGATGGTGTTGTATGTGG‑Cgtcg             <  1:3118394/100‑4 (MQ=255)                                                                           attgtaATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCGAGATGTTGGGGATATAGATGGTGTTGTATGTGG‑CGAGatcggaaga     >  1:4633507/1‑93 (MQ=255)                                                                             tgtaATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCGAGATGTTGGGGATATAGATGGTGTTGTATGTGGTCGAGatcggaagag    >  1:4255808/1‑92 (MQ=255)                                                                             ggtaATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCGAGATGTTGGGGATATAGATGGTGTTGTATGTGG‑CGAGatcggaagagc   >  1:2671969/2‑91 (MQ=255)                                                                              gtaATGTCGTGGAAATTTTACGGGCATTGCACCTGAAGTTGTTATGTGGCGAGATGTTGGGGATATAGATGGTGTTGTATGTGG‑CGAGATATTGGCGCAc  >  1:4707480/1‑100 (MQ=255)                                                                                                  |                                                                                 GGGAACTTAGCTAAAGTTAATTTGTAGTGTGATGTCGTGGAAATATTATGGAGTTATACCAGAAGTTGATTTGTATTGTAATGTCGTGGAAATTTTATGGGCATTGCACCTGAAGTTGTTATGTGGCGAGATGTTGGGGATATAGATGGTGTTGTATGTGG‑CGAGATATTGGCGCAC  >  NC_007793/484518‑484694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑