Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	484,442	G→T	intergenic (+6/‑172)	SAUSA300_RS02305 → / → SAUSA300_RS02310	carboxylesterase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	484,442	0	G	T	100.0%	104.3 / NA	36	intergenic (+6/‑172)	SAUSA300_RS02305/SAUSA300_RS02310	carboxylesterase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (33/3);  total (33/3)

GAAGGATTCGCATCATTTAATGACGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAAGATTTTAAAAATTAACACTTGAGTGACGATGTTATTATATTTGTCATTTGAGTGTTTTTTGTGGCGTGATAGTATAGGGAACTTAGCT  >  NC_007793/484349‑484529                                                                                              |                                                                                        gAAGGATTCGCATCATTTAATGACGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTa                                                                                   >  1:115949/1‑100 (MQ=255)              taatggccgACGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACCCtt                                                                      >  1:3760185/9‑100 (MQ=255)                   aaTGACGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAgat                                                                 >  1:45626/1‑98 (MQ=255)                   aaTGACGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAgat                                                                 >  1:4243416/1‑98 (MQ=255)                   aaTGACGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAgat                                                                 >  1:2203896/1‑98 (MQ=255)                    ctGACGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGa                                                                >  1:2900853/2‑100 (MQ=255)                    ccgACGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAgatc                                                                >  1:2738316/3‑97 (MQ=255)                     tGACGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGAc                                                               >  1:4723550/1‑100 (MQ=255)                     ccaCGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAgatcg                                                               >  1:1676487/3‑96 (MQ=255)                     aaaCGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAgatcg                                                               >  1:3726794/3‑96 (MQ=255)                      aaCGCATGGAGAAGGCCTCTCTCTTTTATAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACg                                                              >  1:3211918/2‑100 (MQ=255)                       ccGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAgatcgga                                                             >  1:4037947/2‑94 (MQ=255)                       aCGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAgatcgga                                                             >  1:2136572/1‑94 (MQ=255)                       aCGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGa                                                             >  1:2340304/1‑100 (MQ=255)                        agCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAgatcggaa                                                            >  1:3184595/2‑93 (MQ=255)                         gtgtGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGATg                                                           >  1:1751198/4‑100 (MQ=255)                         gCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAgatcggaag                                                           >  1:3677683/1‑92 (MQ=255)                         gCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTAGaagatcggaa                                                           >  1:5051933/1‑91 (MQ=255)                          cATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGaagatcggaag                                                          >  1:2707911/1‑90 (MQ=255)                          cATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAgatcggaaga                                                          >  1:4149775/1‑91 (MQ=255)                          cATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGATGt                                                          >  1:2014211/1‑100 (MQ=255)                               taaaGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGATGttatta                                                     >  1:1421682/3‑100 (MQ=255)                               agaAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGATGttatta                                                     >  1:1904707/1‑100 (MQ=255)                                taaGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGATGTtattat                                                    >  1:1866673/2‑100 (MQ=255)                                 aaGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGaagatcggaag                                                   >  1:3023693/1‑90 (MQ=255)                                 aaGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGATGTTATtata                                                   >  1:3625264/1‑100 (MQ=255)                                 aaGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGATGTTATtata                                                   >  1:2531372/1‑100 (MQ=255)                                 aaGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGATGTTATtata                                                   >  1:2330543/1‑100 (MQ=255)                                 aaGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGATGTTATtata                                                   >  1:4283779/1‑100 (MQ=255)                                 aaGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGATGTTATtata                                                   >  1:1581976/1‑100 (MQ=255)                                 aaGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGATGTTATtata                                                   >  1:93356/1‑100 (MQ=255)                                  aGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGATGTTATTatat                                                  >  1:3433813/1‑100 (MQ=255)                                                               ttCGCTTTTTCAATGCTTTAACATAATTGGATATTTTAAAGATTAACACTTGAGTGACGATGTTATTATATTTGTCATTTGAGTGTTTTTTGTGGCGtcg                     <  1:4646607/100‑3 (MQ=255)                                                                tCGCTTTTTCAATGCTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGATGTTATTATATTTGTCATTTGAGTGTTTTTTGTGGCGTGAt                    <  1:1614624/100‑1 (MQ=255)                                                                              cTTTAACATAATTGAATATTTTAAAAATTAACACTTGAGTGACGATGTTATTATATTTGTCATTTGAGTGTTTTTTGTAGCGTGATAGTATAGGGAACtt      <  1:4461789/100‑1 (MQ=255)                                                                                  aaCATAATTGAATATTTTAAAAATTAACACTTGAGTGACGATGTTATTATATTTGTCATTTGAGTGTTTTTTGTGGCGTGATAGTATAGGGAACTTAGCt  >  1:1546432/1‑100 (MQ=255)                                                                                              |                                                                                        GAAGGATTCGCATCATTTAATGACGCATGGAGAAGGCAGAGATATTTTAGAAGAAAATGTTATTCGCTTTTTCAATGCTTTAACATAATTGAAGATTTTAAAAATTAACACTTGAGTGACGATGTTATTATATTTGTCATTTGAGTGTTTTTTGTGGCGTGATAGTATAGGGAACTTAGCT  >  NC_007793/484349‑484529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑