D66 :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	479,868	T→C	Y264H (TAC→CAC)	SAUSA300_RS02285 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	479,868	0	T	C	99.9%	4301.5 / NA	1562	Y264H (TAC→CAC)	SAUSA300_RS02285	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (1548/11); total (1551/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 1562 total aligned reads displayed.

TTCAATAGATCAGGAAGACTACCAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATTACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAGTTGGTGATGAGTTTAAGTCAGTCGCTAAAGA  >  NC_007793/479771‑479962

               aGACTACCAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAGCATTTg                                                                               >  1:415860/1‑100 (MQ=255)

                    aCCAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAAc                                                                          >  1:3853715/1‑100 (MQ=255)

                    aCCAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAAc                                                                          >  1:2621244/1‑100 (MQ=255)

                    aCCAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAAc                                                                          >  1:4429790/1‑100 (MQ=255)

                    aCCAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAAc                                                                          >  1:3366090/1‑100 (MQ=255)

                    aCCAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAAc                                                                          >  1:1316490/1‑100 (MQ=255)

                    aCCAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAAc                                                                          >  1:5746018/1‑100 (MQ=255)

                    aCCAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAAc                                                                          >  1:905671/1‑100 (MQ=255)

                    aCCAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAAc                                                                          >  1:1506571/1‑100 (MQ=255)

                    aCCAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAAc                                                                          >  1:3580329/1‑100 (MQ=255)

                    aCCAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAAc                                                                          >  1:3635901/1‑100 (MQ=255)

                    aCCAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAAc                                                                          >  1:2102139/1‑100 (MQ=255)

                    aCCAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAAc                                                                          >  1:3696651/1‑100 (MQ=255)

                    aCCAAGCTTATGTTGAAGGACATCTCTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAAc                                                                          >  1:4350770/1‑100 (MQ=255)

                    aCCAAGCTTATATTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAAc                                                                          >  1:5599450/1‑100 (MQ=255)

                      cAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACgg                                                                        >  1:1923099/1‑100 (MQ=255)

                                    aGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAAtt                                                          >  1:4587938/1‑100 (MQ=255)

                                    aGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAAtt                                                          >  1:396494/1‑100 (MQ=255)

                                     acaCATCTTTTGGCGTCACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCaagat                                                         >  1:3768992/3‑97 (MQ=255)

                                            tcctGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGt                                                  >  1:594137/4‑100 (MQ=255)

                                            tactGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGt                                                  >  1:1679134/4‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACCACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:3336201/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAGCATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:4895779/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGTCAATTCGGTTAGtt                                                 >  1:3528348/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:3094585/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:3804687/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:3140756/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:3165084/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:5480765/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:322286/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:3272000/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:4531169/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:4690295/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:3397910/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:5065760/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:5009391/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:421420/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:3915208/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:5162921/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:1269502/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:1364348/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:1607280/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:1788726/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:1955924/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:1204373/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:204541/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:2275066/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:2379084/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:2484553/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:2568396/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:1092160/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:1001158/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:5412440/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACCTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:1561169/1‑100 (MQ=255)

                                             tttGGCGTTACCGGGTTGGGCAGGTATGTAGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:4824592/1‑100 (MQ=255)

                                             tttGACGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:1140224/1‑100 (MQ=255)

                                             cttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:1848205/2‑100 (MQ=255)

                                             cttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:1750782/2‑100 (MQ=255)

                                             attGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGtt                                                 >  1:252676/2‑100 (MQ=255)

                                              ttGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTg                                                >  1:2671411/1‑100 (MQ=255)

                                              ctGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTg                                                >  1:5647919/2‑100 (MQ=255)

                                              ctGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTg                                                >  1:270483/2‑100 (MQ=255)

                                              ctGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCAGTTAGTTg                                                >  1:4487333/2‑100 (MQ=255)

                                               tGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGt                                               >  1:2961412/1‑100 (MQ=255)

                                               tGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGt                                               >  1:4244796/1‑100 (MQ=255)

                                               tGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGt                                               >  1:857426/1‑100 (MQ=255)

                                               tGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGCTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGt                                               >  1:2005468/1‑100 (MQ=255)

                                                   gTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGaa                                           >  1:3732683/1‑100 (MQ=255)

                                                   gTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGaa                                           >  1:2753249/1‑100 (MQ=255)

                                                    ttACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAAc                                          >  1:4642195/1‑100 (MQ=255)

                                                     tACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTGGTTGTCGAACa                                         >  1:956764/1‑100 (MQ=255)

                                                     tACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACa                                         >  1:826815/1‑100 (MQ=255)

                                                     tACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACa                                         >  1:4028754/1‑100 (MQ=255)

                                                      ggtgggTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACaa                                        >  1:4752961/4‑100 (MQ=255)

                                                      cccGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACaa                                        >  1:700883/2‑100 (MQ=255)

                                                       tgtggTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAAt                                       >  1:2342491/4‑100 (MQ=255)

                                                          gtatGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGc                                    >  1:3023111/4‑100 (MQ=255)

                                                          ggTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGc                                    >  1:2919499/1‑100 (MQ=255)

                                                          ctttGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGc                                    >  1:1427510/3‑100 (MQ=255)

                                                           cttGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCt                                   >  1:643903/2‑100 (MQ=255)

                                                           cttGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCt                                   >  1:4953032/2‑100 (MQ=255)

                                                           cgtGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCt                                   >  1:5101614/3‑100 (MQ=255)

                                                           actGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAGCAATTGCt                                   >  1:2864358/3‑100 (MQ=255)

                                                            ttGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTa                                  >  1:2819766/1‑100 (MQ=255)

                                                            ttGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTa                                  >  1:5243454/1‑100 (MQ=255)

                                                            ttGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTa                                  >  1:3486002/1‑100 (MQ=255)

                                                             tGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAg                                 >  1:2212860/1‑100 (MQ=255)

                                                             tGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAg                                 >  1:5204428/1‑100 (MQ=255)

                                                             tGGGCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAg                                 >  1:217367/1‑100 (MQ=255)

                                                              gggCAGGTATGTTGTATTGCCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAGt                                >  1:5685943/1‑100 (MQ=255)

                                                              gggCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAGt                                >  1:535901/1‑100 (MQ=255)

                                                              gggCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAGt                                >  1:775190/1‑100 (MQ=255)

                                                              gggCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAGt                                >  1:2431315/1‑100 (MQ=255)

                                                              gggCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAGt                                >  1:343487/1‑100 (MQ=255)

                                                              gggCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAGt                                >  1:4086543/1‑100 (MQ=255)

                                                              gggCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAGt                                >  1:4380919/1‑100 (MQ=255)

                                                              aggCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAGt                                >  1:5524775/2‑100 (MQ=255)

                                                               ggCAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAGtc                               >  1:429556/1‑99 (MQ=255)

                                                                 cAGGTATGTTGTATTACCGTTCACAACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAGTTgg                             >  1:5312897/1‑100 (MQ=255)

                                                                                      cacaACAGCATCACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAGTTGGTGATGAGTTTAAGTCAGTCGc        >  1:1060557/1‑100 (MQ=255)

TTCAATAGATCAGGAAGACTACCAAGCTTATGTTGAAGGACATCTTTTGGCGTTACCGGGTTGGGCAGGTATGTTGTATTACCGTTCACAACAGCATTACTTTGAACAACATTTGTTAACGGATTATTTGGCAATTCGGTTAGTTGTCGAACAATTGCTAGTTGGTGATGAGTTTAAGTCAGTCGCTAAAGA  >  NC_007793/479771‑479962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`