D66 :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	478,396	G→T	G272V (GGT→GTT)	SAUSA300_RS02280 →	NADH dehydrogenase subunit 5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	478,396	0	G	T	100.0%	403.2 / NA	133	G272V (GGT→GTT)	SAUSA300_RS02280	NADH dehydrogenase subunit 5
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (129/4); total (129/4)

Only 100 of 133 total aligned reads displayed.

TTATTCTAACTCGTTTTGCGCCGATATTTGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGGTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTGGGTGTATA  >  NC_007793/478303‑478492

aacctCTAACTCGTTTTGCGCCGATATTTGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAg                                                                                            >  1:5569276/5‑100 (MQ=255)

       aaCTCGTTTTGCGCCGATATTTGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGtt                                                                                     >  1:2907324/1‑100 (MQ=255)

       aaCTCGTTTTGCGCCGATATTTGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGtt                                                                                     >  1:4008526/1‑100 (MQ=255)

               ttGCGCCGATATTTGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGa                                                                             >  1:5349262/1‑100 (MQ=255)

                tGCGCCGATATTTGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGAc                                                                            >  1:2394981/1‑100 (MQ=255)

                 ccgcCGATATTTGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACt                                                                           >  1:1712928/2‑100 (MQ=255)

                  cgcCGATATTTGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACta                                                                          >  1:4249645/1‑100 (MQ=255)

                   gcCGATATTTGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACtat                                                                         >  1:80413/1‑100 (MQ=255)

                           ttGATAATGGATTTGTGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:1022409/1‑100 (MQ=255)

                           ttGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTCTTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGcct                                                                 >  1:4660344/1‑99 (MQ=255)

                           ttGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:5487623/1‑100 (MQ=255)

                           ttGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:4537805/1‑100 (MQ=255)

                           ttGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:901274/1‑100 (MQ=255)

                           ttGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:3921784/1‑100 (MQ=255)

                           ttGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:3909228/1‑100 (MQ=255)

                           ttGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:3373789/1‑100 (MQ=255)

                           ttGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:3254332/1‑100 (MQ=255)

                           ttGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:263670/1‑100 (MQ=255)

                           ttGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:2521326/1‑100 (MQ=255)

                           ttGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:247981/1‑100 (MQ=255)

                           ttGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:3691354/1‑100 (MQ=255)

                           ttGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:1547052/1‑100 (MQ=255)

                           ttGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:1815946/1‑100 (MQ=255)

                           ttGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATAGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:4112221/1‑100 (MQ=255)

                           gtttctttGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCa                                                                 >  1:2793690/8‑100 (MQ=255)

                             gATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAAt                                                               >  1:3600035/1‑100 (MQ=255)

                                                      taCTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAAt                                      >  1:3350226/1‑100 (MQ=255)

                                                              cTTTCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTa                              >  1:4824196/1‑100 (MQ=255)

                                                                ttCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATg                            >  1:3081867/1‑100 (MQ=255)

                                                                ttCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATg                            >  1:2750536/1‑100 (MQ=255)

                                                                ttCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATg                            >  1:2165233/1‑100 (MQ=255)

                                                                 tCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGt                           >  1:4179459/1‑100 (MQ=255)

                                                                 tCTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGt                           >  1:753893/1‑100 (MQ=255)

                                                                 ccTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGt                           >  1:4687530/2‑100 (MQ=255)

                                                                  cTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGtt                          >  1:1020710/1‑100 (MQ=255)

                                                                  cTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGtt                          >  1:2178258/1‑100 (MQ=255)

                                                                  cTAGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCCACGATGAGTCAAATGGGCTTTATGtt                          >  1:3574248/1‑100 (MQ=255)

                                                                    aGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAg                        >  1:2697533/1‑100 (MQ=255)

                                                                    aGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAg                        >  1:377150/1‑100 (MQ=255)

                                                                    aGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAg                        >  1:5054305/1‑100 (MQ=255)

                                                                    aGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAg                        >  1:853127/1‑100 (MQ=255)

                                                                    aGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAg                        >  1:2591737/1‑100 (MQ=255)

                                                                    aGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAg                        >  1:1490696/1‑100 (MQ=255)

                                                                    aGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAg                        >  1:4084793/1‑100 (MQ=255)

                                                                    aGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAg                        >  1:2066934/1‑100 (MQ=255)

                                                                    aGTATTTCTGTATTGTTAGGATCGGTTATTAGCTTAGTCCAAGTTGACTATAAACGCCAATTAGGGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAg                        >  1:5580370/1‑100 (MQ=255)

                                                                         ttCTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCaa                   >  1:754998/1‑100 (MQ=255)

                                                                          ccTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAt                  >  1:5683678/2‑100 (MQ=255)

                                                                           ttggggggTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtg                 >  1:5335953/8‑100 (MQ=255)

                                                                           cTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAGTGGGCTTTATGTTAGTTCAAtg                 >  1:5373303/1‑100 (MQ=255)

                                                                           cTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtg                 >  1:2329053/1‑100 (MQ=255)

                                                                           cTGTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtg                 >  1:1268319/1‑100 (MQ=255)

                                                                             gtcttGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGTCTATAAACGCCAATTAGTGGGCTCTACGCTGCGTCAAATGGGCTTTATGATTGTTCAGtgtg               >  1:5568890/4‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGCGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:3436270/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:4911020/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:5171149/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:1578377/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:5283510/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:4316992/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:42378/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:963929/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:1157792/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:2361238/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:4113580/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:323905/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:3274680/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:3062439/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:2017096/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:352613/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:2595991/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:3541267/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:2249818/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:3609826/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:3788487/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:3875712/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:2475839/1‑100 (MQ=255)

                                                                             gTATTGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAAtgtg               >  1:1923550/1‑100 (MQ=255)

                                                                                ttGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCAt            <  1:366973/100‑1 (MQ=255)

                                                                                 tcttccgATCTGTTACTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCAtt           <  1:3467333/94‑1 (MQ=255)

                                                                                 tGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCAtt           >  1:3128690/1‑100 (MQ=255)

                                                                                 tGTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCAtt           >  1:304503/1‑100 (MQ=255)

                                                                                  gctAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTg          >  1:1612777/3‑100 (MQ=255)

                                                                                  gTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTg          >  1:4977843/1‑100 (MQ=255)

                                                                                  gTTAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGC‑TTATGTTAGTTCAATGTGCATTgg         >  1:656429/1‑100 (MQ=255)

                                                                                  gTTAGGATCGGTTATTAGCTTAGTTCAAGGTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTg          >  1:786173/1‑100 (MQ=255)

                                                                                  cttAGGATCGGTTATTAGCTTAGTTCTAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTg          >  1:4760077/2‑100 (MQ=255)

                                                                                  ctatggATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTg          >  1:4051068/5‑100 (MQ=255)

                                                                                  cgtAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTg          >  1:459310/3‑100 (MQ=255)

                                                                                  cctAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTg          >  1:1146735/3‑100 (MQ=255)

                                                                                   gcaGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTgg         >  1:377450/3‑100 (MQ=255)

                                                                                   ctAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTgg         >  1:3701834/2‑100 (MQ=255)

                                                                                    tAGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTggg        >  1:4212585/1‑100 (MQ=255)

                                                                                    caGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTggg        >  1:5223385/2‑100 (MQ=255)

                                                                                     aGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTGGgt       >  1:3163280/1‑100 (MQ=255)

                                                                                     aGGATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTGGgt       >  1:1704300/1‑100 (MQ=255)

                                                                                      ggATCGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTGGgtg      >  1:5320869/1‑100 (MQ=255)

                                                                                       ccccGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTGGgtgt     >  1:5297925/4‑100 (MQ=255)

                                                                                          cGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTGGGTGtata  >  1:146079/1‑100 (MQ=255)

                                                                                          cGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTGGGTGtata  >  1:5211792/1‑100 (MQ=255)

                                                                                          cGGTTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGAGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTGGGTGtata  >  1:5331823/1‑100 (MQ=255)

TTATTCTAACTCGTTTTGCGCCGATATTTGATAATGGATTTGCGTTATCATTATTACTTATCCTTTCTAGTATTTCTGTATTGTTAGGATCGGGTATTAGCTTAGTTCAAGTTGACTATAAACGCCAATTAGTGGGCTCTACGATGAGTCAAATGGGCTTTATGTTAGTTCAATGTGCATTGGGTGTATA  >  NC_007793/478303‑478492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`