Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	46,305	T→G	I406L (ATA→CTA)	SAUSA300_RS00200 ←	recombinase RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	46,305	0	T	G	100.0%	29.6 / NA	13	I406L (ATA→CTA)	SAUSA300_RS00200	recombinase RecB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (5/8);  total (5/8)

TTGAATACTTGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCGATGGTTTTAATCAAGTTATTTATTTTGATTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTGATTTTCTTGATTGACGTGTGTTAC  >  NC_007793/46237‑46401                                                                     |                                                                                                 ttGAATACTTGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGc                                                                   <  1:1157303/100‑1 (MQ=255)   gctcttccgatCTGATTACTGATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCtt                                                                 <  1:151088/90‑1 (MQ=255)   gAATACTTGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCtt                                                                 <  1:2728753/100‑1 (MQ=255)      cttccgatCTGATTACTGATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTtata                                                              <  1:4538772/93‑1 (MQ=255)          tGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCa                                                          <  1:4569899/100‑1 (MQ=255)                gATTACTGATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCg                                                    >  1:2346698/1‑100 (MQ=255)                 aTTACTGATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTAGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGt                                                   <  1:3334506/100‑1 (MQ=255)                       gATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGaa                                             >  1:2996864/1‑100 (MQ=255)                       gATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGaa                                             >  1:3552329/1‑100 (MQ=255)                             aaGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTATACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAg                                       >  1:5379926/1‑100 (MQ=255)                                     aTTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCa                               <  1:5024940/100‑1 (MQ=255)                                         tCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACta                           <  1:4140197/100‑2 (MQ=255)                                          ccTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTTGTAAGCAATATCGTGATGAAGTGCAGCGCCATCAACttt                          >  1:4801495/1‑99 (MQ=255)                                                                atttagttTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAAcacgtgct    <  1:4463483/100‑8 (MQ=255)                                                                  ttagttTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCtac  <  1:215338/100‑1 (MQ=255)                                                                  ttagttTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTTATTTTCTTGATTAACACGTGCtac  <  1:963282/100‑1 (MQ=255)                                                                     |                                                                                                 TTGAATACTTGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCGATGGTTTTAATCAAGTTATTTATTTTGATTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTGATTTTCTTGATTGACGTGTGTTAC  >  NC_007793/46237‑46401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑