Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	463,755	T→A	I44K (ATA→AAA)	SAUSA300_RS02200 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	463,755	0	T	A	100.0%	12.3 / NA	6	I44K (ATA→AAA)	SAUSA300_RS02200	lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (6/0);  total (6/0)

TTGCGATAGCAGGTTGTGGCAAAGGTAATGAAACAAAAGAAGATTCAAAGGAAGAACAAATCAAAAAGAGCTTTGCGATAACATTAGATATGTATCCAATTAAGAATCTCGAGGAC  >  NC_007793/463677‑463792                                                                               |                                      atGCGATAGCAGGTTGTGGCAAAGGTAATGAAACAAAAGAAGATTCAAAGGAAGAACAAATCAAAAAGAGCTTTGCGAAAACATTAGATATGTATCCAAt                  >  1:5389517/2‑100 (MQ=255)      aTAGCAGGTTGTGGCAAAGGTAATGAAACAAAAGAAGATTCAAAGGAAGAACAAATCAAAAAGAGCTTTGCGAAAACATTAGATATGTATCCAATTAAGa             >  1:298894/1‑100 (MQ=255)      aTAGCAGGTTGTGGCAAAGGTAATGAAACAAAAGAAGATTCAAAGGAAGAACAAATCAAAAAGAGCTTTGCGAAAACATTAGATATGTATCCAATTAAGa             >  1:5774595/1‑100 (MQ=255)               tgtgGCAAAGGTAATGAAACAAAAGAAGATTCAAAGGAAGAACAAATCAAAAAGAGCTTTGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAgg    >  1:2153068/1‑100 (MQ=255)                 tgGCAAAGGTAATGAAACAAAAGAAGATTCAAAGGAAGAACAAATCAAAAAGAGCTTTGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAGGAc  >  1:2689111/1‑100 (MQ=255)                 tgGCAAAGGTAATGAAACAAAAGAAGATTCAAAGGAAGAACAAATCAAAAAGAGCTTTGCGAAAACATTAGATATGTATCCAATTAAGAATCTCGAGGAc  >  1:4334373/1‑100 (MQ=255)                                                                               |                                      TTGCGATAGCAGGTTGTGGCAAAGGTAATGAAACAAAAGAAGATTCAAAGGAAGAACAAATCAAAAAGAGCTTTGCGATAACATTAGATATGTATCCAATTAAGAATCTCGAGGAC  >  NC_007793/463677‑463792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑