Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	444,915	C→T	G63R (GGA→AGA)	SAUSA300_RS02100 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	444,915	0	C	T	100.0%	27.6 / NA	11	G63R (GGA→AGA)	SAUSA300_RS02100	membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)

ACCATAAATAGCAACGCCAATGATATACACTAAAATAGCTGCGCATATTTCTTTTGGATTACTGCTGATAAACAAACCGTATATTAGCAAAACTCCGATTAAACCGTTATATACGCCTTGGTTCTTCAAAAGTAGGTTAATATTTTTGTCTTTCAATTTATCGACGCTTATAT  >  NC_007793/444820‑444992                                                                                                |                                                                              accaTAAATAGCAACGCCAATGATATACACTAAAATAGCTGCGCATATTTCTTTTGGATTACTGCTGATAAACAAACCGTATATTAGCAAAACTCTGAtt                                                                           <  1:2314616/100‑1 (MQ=255) accaTAAATAGCAACGCCAATGATATACACTAAAATAGCTGCGCATATTTCTTTTGGATTACTGCTGATAAACAAACCGTATATTAGCAAAACTCTGAtt                                                                           <  1:300306/100‑1 (MQ=255)         tAGCAACGCCAATGATATACACTAAAATAGCTGCGCATATTTCTTTTGGATTACTGCTGATAAACAAACCGTATATTAGCAAAACTCTGATTAAACCGtt                                                                   <  1:3137300/100‑1 (MQ=255)         tAGCAACGCCAATGATATACACTAAAATAGCTGCGCATATTTCTTTTGGATTACTGCTGATAAACAAACCGTATATTAGCAAAACTCTGATTAAACCGtg                                                                   <  1:5043961/100‑2 (MQ=255)               cGCCAATGATATACACTAAAATAGCTGCGCATATTTCTTTTGGATTACTGCTGATAAACAAACCGTATATTAGCAAAACTCTGATTAAACCGTTATATAc                                                             >  1:1928266/1‑100 (MQ=255)                     tGATATACACTAAAATAGCTGCGCATATTTCTTTTGGATTACTGCTGATAAACAAACCGTATATTAGCAAAACTCTGATTAAACCGTTATATACGCCTTg                                                       <  1:1698233/100‑1 (MQ=255)                                         aggcATATTTCTTTTGGATTACTGCTGATAAACAAACCGTATATTAGCAAAACTCTGATTAAACCGTTATATACGCCTTGGTTCTTCAAAAGTAGGTTaa                                   >  1:825712/3‑100 (MQ=255)                                                              ctgctgATAAACAAACCGTATATTAGCAAAACTCTGATTAAACCGTTATATACGCCTTGGTTCTTCAAAAGCAGGTTAATATTTTTGTCTTTCAATTTAt              >  1:3753008/1‑100 (MQ=255)                                                                gctgATAAACAAACCGTATATTAGCAAAACTCTGATTAAACCGTTATATACGCCTTGGTTCTTCAAAAGTAGGTTAATATTTTTGTCTTTCAATTCATCg            <  1:1892025/100‑1 (MQ=255)                                                                       aacaaacCGTATATTAGCAAAACTCTGATTAAACCGTTATATACGCCTTGGTTCTTCAAAAGTAGGTTAATATTTTTGTCTTTCAATTTATCGACGCTta     >  1:5241942/1‑100 (MQ=255)                                                                          caacCGTATATTAGCAAAACTCTGATTAAACCGTTATATACGCCTTGGTTCTTCAAAAGTAGGTTAATATTTTTGTCTTTCAATTTATCGACGCTTatat  >  1:3602276/2‑100 (MQ=255)                                                                                                |                                                                              ACCATAAATAGCAACGCCAATGATATACACTAAAATAGCTGCGCATATTTCTTTTGGATTACTGCTGATAAACAAACCGTATATTAGCAAAACTCCGATTAAACCGTTATATACGCCTTGGTTCTTCAAAAGTAGGTTAATATTTTTGTCTTTCAATTTATCGACGCTTATAT  >  NC_007793/444820‑444992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑