Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	444,723	C→A	intergenic (‑505/+19)	SAUSA300_RS02095 ← / ← SAUSA300_RS02100	hypothetical protein/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	444,723	0	C	A	100.0%	34.7 / NA	14	intergenic (‑505/+19)	SAUSA300_RS02095/SAUSA300_RS02100	hypothetical protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/4);  total (10/4)

GCTTGAAATAATTAGGATGAAATACTCTGAGTCTAAAGTCCACACACAATTCGCTCAAATCCTCCAAACCTCCGACTACATCTCCACCCCCCCAAACACCAATACTTACCAAAGCATTGATATGAGTGCCAATACTGGCAATGTGCCTTGTTTGAAAAAGATACTAATATTGCTTGAAAGGCC  >  NC_007793/444637‑444819                                                                                       |                                                                                                 gCTTGAAATAATTAGGATGAAATACTCTGAGTCTAAAGTCCACACACAATTCGCTCAAATCCTCCAAACCTCCGACTACATCTCCAACCCCCCAAACAcc                                                                                     >  1:5071389/1‑100 (MQ=255)    tGAAATAATTAGGATGAAATACTCTGAGTCTAAAGTCCACACACAATTCGCTCAAATCCTCCAAACCTCCGACTACATCTCCAACCCCCCAAACACCAAt                                                                                  <  1:3295753/100‑1 (MQ=255)    tGAAATAATTAGGATGAAATACTCTGAGTCTAAAGTCCACACACAATTCGCTCAAATCCTCCAAACCTCCGACTACATCTCCAACCCCCCAAACACCAAt                                                                                  >  1:4761579/1‑100 (MQ=255)        ataatTAGGATGAAATACTCTGAGTCTAAAGTCCACACACAATTCGCTCAAATCCTCCAAACCTCCGACTACATCTCCAACCCCCCAAACACCAATACtt                                                                              <  1:1758245/100‑1 (MQ=255)                 aTGAAATACTCTGAGTCTAAAGTCCACACACAATTCGCTCAAATCCTCCAAACCTCCGACTACATCTCCAACCCCCCAAACACCAATACTTACCAAAGCa                                                                     >  1:5427967/1‑100 (MQ=255)                                         cacacacaATTCGCTCAAATCCTCCAAACCTCCGACTACATCTCCAACCCCCCAAACACCAATACTTACCAAAGCATTGATATGAGTGCCAATACTGGCa                                             >  1:1784424/1‑100 (MQ=255)                                           cacacaATTCGCTCAAATCCTCCAAACCTCCGACTACATCTCCAACCCCCCAAACACCAATACTTACCAAAGCATTGATATGAGTGCCAATACTGGCAAt                                           >  1:2219055/1‑100 (MQ=255)                                                   tCGCTCTAATCCTCCAAACCTCCGACTACATCTCCAACCCCCCAAACACCAATACTTACCAAAGCATTGATATGAGTGCCAATACTGGCAATGTGCCtta                                   <  1:5714050/100‑2 (MQ=255)                                                                ccAAACCTCCGACTACATCTCCAACCCCCCAAACACCAATACTTACCAAAGCATTGATATGAGTGCCAATACTGGCAATGTGCCTTGTTTGAAAAAGATa                      >  1:3694863/1‑100 (MQ=255)                                                                ccAAACCTCCGACTACATCTCCAACCCCCCAAACACCAATACTTACCAAAGCATTGATATGAGTGCCAATACTGGCAATGTGCCTTGTTTGAAAAAGATa                      >  1:4060895/1‑100 (MQ=255)                                                                  aaaCCTCCGACTACATCTCCAACCCCCCAAACACCAATACTTACCAAAGCATTGATCTGAGTGCCAATACTGGCAATGTGCCTTGTTTGAAAAAGATACt                    >  1:4253763/1‑100 (MQ=255)                                                                              aCATCTCCAACCCCCCAAACACCAATACTTACCAAAGCATTGATATGAGTGCCAATACTGGCAATGTGCCTTGTTTGAAAAAGATACTAATATTGCTTGa        <  1:3888748/100‑1 (MQ=255)                                                                                    ccAACCCCCCAAACACCAATACTTACCAAAGCATTGATATGAGTGCCAATACTGGCAATGTGCCTTGTTTGAAAAAGATACTAATATTGCTTGAAAGGcc  >  1:465943/1‑100 (MQ=255)                                                                                    ccAACCCCCCAAACACCAATACTTACCAAAGCATTGATATGAGTGCCAATACTGGCAATGTGCCTTGTTTGAAAAAGATACTAATATTGCTTGAAAGGcc  >  1:4716383/1‑100 (MQ=255)                                                                                    ccAACCCCCCAAACACCAATACTTACCAAAGCATTGATATGAGTGCCAATACTGGCAATGTGCCTTGTTTGAAAAAGATACTAATATTGCTTGAAAGGcc  <  1:5516445/100‑1 (MQ=255)                                                                                       |                                                                                                 GCTTGAAATAATTAGGATGAAATACTCTGAGTCTAAAGTCCACACACAATTCGCTCAAATCCTCCAAACCTCCGACTACATCTCCACCCCCCCAAACACCAATACTTACCAAAGCATTGATATGAGTGCCAATACTGGCAATGTGCCTTGTTTGAAAAAGATACTAATATTGCTTGAAAGGCC  >  NC_007793/444637‑444819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑