Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	431,328	C→T	A457T (GCT→ACT)	SAUSA300_RS02035 ←	L‑cystine transporter tcyP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	431,328	0	C	T	100.0%	71.1 / NA	27	A457T (GCT→ACT)	SAUSA300_RS02035	L‑cystine transporter tcyP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/6);  total (21/6)

ATAAGTATAGCTAGAGGGGGCGGGTATTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGCATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTACCTGTACCAGCTAGCATTGAATCGTTCACGTTTAATGCTG  >  NC_007793/431268‑431423                                                             |                                                                                                atctgTATAGCTAGAGGGGGCGGGTATTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGttt                                                          <  1:1987948/96‑1 (MQ=255)  tAAGTATAGCTAGAGGGGGCGGGTATTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTa                                                         >  1:2838298/1‑100 (MQ=255)     ttataGCTAGAGGGGGCGGGTATTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGtt                                                      >  1:2371280/2‑100 (MQ=255)     caataGCTAGAGGGGGCGGGTATTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGtt                                                      >  1:2671181/3‑100 (MQ=255)      cataGCTAGAGGGGGCGGGTATTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTa                                                     >  1:1199173/2‑100 (MQ=255)      cataGCTAGAGGGGGCGGGTATTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTa                                                     >  1:1215439/2‑100 (MQ=255)      cataGCTAGAGGGGGCGGGTATTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTa                                                     >  1:4223685/2‑100 (MQ=255)      cataGCTAGAGGGGGCGGGTATTTCTTGCAATGAATTAGTGTGAAGTTAATACAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTa                                                     >  1:5360236/2‑100 (MQ=255)       ataGCTAGAGGGGGCGGGTATTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTaa                                                    >  1:3786019/1‑100 (MQ=255)         aGCTAGAGGGGGCGGGTATTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACt                                                  >  1:5247851/1‑100 (MQ=255)         aGCTAGAGGGGGCGGGTATTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACt                                                  >  1:3001871/1‑100 (MQ=255)             agagGGGGCGGGTATTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGc                                              >  1:792882/1‑100 (MQ=255)                   ggCGGGTATTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTAcc                                        >  1:3629057/1‑100 (MQ=255)                    gCGGGTGTTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTACCt                                       <  1:4305382/100‑1 (MQ=255)                        gTATTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTACCTGTag                                   <  1:2061530/100‑2 (MQ=255)                             tCTTGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTACCTGTACCAGCt                              <  1:5360945/100‑1 (MQ=255)                                tGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTACCTGTACCAGCTAGc                           >  1:2519691/1‑100 (MQ=255)                                tGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTACCTGTACCAGCTAGc                           >  1:2184416/1‑100 (MQ=255)                                tGCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTACCTGTACCAGCTAGc                           >  1:651153/1‑100 (MQ=255)                                tGCAACGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTACCTGTACCAGCTAGc                           >  1:3991914/1‑100 (MQ=255)                                 gCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAATTTGGCGGTACCTGTACCAGCTAGCa                          >  1:2208322/1‑100 (MQ=255)                                 gCAATGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTACCTGTACCAGCTAGCa                          >  1:5304626/1‑100 (MQ=255)                                   aaTGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTACCTGTACCAGCTAGCAtt                        <  1:2907408/100‑1 (MQ=255)                                    aTGAATTAGTGTGAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGGACCTGTACCAGCTAGCATTg                       >  1:4792248/1‑100 (MQ=255)                                               tgAAGTTAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTACCTGTACCAGCTAGCATTGAATCGTTCAca            <  1:4078079/100‑2 (MQ=255)                                                     tAATGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTACCTGTACCAGCTAGCATTGAATCGTTCACGTTTAAt      >  1:4095323/1‑100 (MQ=255)                                                      aaTGCAGTATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTACCTGTACCAGCTAGCATTGAATCGTTCACGTTTAATg     >  1:1585829/1‑100 (MQ=255)                                                         gcagtaTTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTACCTGTACCAGCTAGCATTGAATCGTTCACGTTTAATGCTg  >  1:1017185/1‑100 (MQ=255)                                                             |                                                                                                ATAAGTATAGCTAGAGGGGGCGGGTATTTCTTGCAATGAATTAGTGTGAAGTTAATGCAGCATTATCATTTGAATCGAAAGTATCTTTATCCCAATGTTTAGTTAACTTGGCGGTACCTGTACCAGCTAGCATTGAATCGTTCACGTTTAATGCTG  >  NC_007793/431268‑431423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑