Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	46,282	G→T	I413I (ATC→ATA)	SAUSA300_RS00200 ←	recombinase RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	46,282	0	G	T	100.0%	50.0 / NA	19	I413I (ATC→ATA)	SAUSA300_RS00200	recombinase RecB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/13);  total (6/13)

TGAGTTGGTTGATTTGATTCGTAATGTCATTGAGTTGCTTTTCATATTTTTGAATACTTGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCGATGGTTTTAATCAAGTTATTTATTTTGATTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTG  >  NC_007793/46188‑46377                                                                                               |                                                                                                tGAGTTGGTTGATTTGATTCGTAATGTCATTGAGTTGCTTTTCATATTTTTGAATACTTGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCTATggg                                                                                            <  1:4244300/100‑2 (MQ=255)  gAGTTGGTTGATTTGATTCGTAATGTCATTGAGTTGCTTTTCATATTTTTGAATACTTGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCTATGGtt                                                                                           <  1:3654039/100‑1 (MQ=255)    gttggttgATTTGATTCGTAATGTCATTGAGTTGCTTTTCATATTTTTGAATACTTGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCTATGGtttt                                                                                         <  1:727100/100‑1 (MQ=255)    gttggttgATTTGATTCGTAATGTCATTGAGTTGCTTTTCATATTTTTGAATACTTGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCTATGGtcac                                                                                         <  1:5359963/100‑4 (MQ=255)      tggttgATTTGATTCGTAATGTCATTGAGTTGCTTTTCATATTTTTGAATACTTGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCTATgggcacaa                                                                                       <  1:4139855/100‑7 (MQ=255)                    cGTAATGTCATTGAGTTGCTTTTCATATTTTTGAATACTTGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGttatttagt                                                                         >  1:2003409/1‑98 (MQ=255)                                                  ttGAATACTTGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGc                                           <  1:1157303/100‑1 (MQ=255)                                                    gctcttccgatCTGATTACTGATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCtt                                         <  1:151088/90‑1 (MQ=255)                                                    gAATACTTGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCtt                                         <  1:2728753/100‑1 (MQ=255)                                                       cttccgatCTGATTACTGATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTtata                                      <  1:4538772/93‑1 (MQ=255)                                                           tGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCa                                  <  1:4569899/100‑1 (MQ=255)                                                                 gATTACTGATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCg                            >  1:2346698/1‑100 (MQ=255)                                                                  aTTACTGATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTAGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGt                           <  1:3334506/100‑1 (MQ=255)                                                                        gATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGaa                     >  1:2996864/1‑100 (MQ=255)                                                                        gATGTTAAGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGaa                     >  1:3552329/1‑100 (MQ=255)                                                                              aaGTCCGGATTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTATACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAg               >  1:5379926/1‑100 (MQ=255)                                                                                      aTTATCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCa       <  1:5024940/100‑1 (MQ=255)                                                                                          tCCTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACta   <  1:4140197/100‑2 (MQ=255)                                                                                           ccTCTATGGTTTTAATCAAGTTATTTAGTTTGGTTTGTACTTCATCATATTGTTGTTGCTTGTAAGCAATATCGTGATGAAGTGCAGCGCCATCAACttt  >  1:4801495/1‑99 (MQ=255)                                                                                               |                                                                                                TGAGTTGGTTGATTTGATTCGTAATGTCATTGAGTTGCTTTTCATATTTTTGAATACTTGGTCTGATTACTGATGTTAAGTCCGGATTATCCTCGATGGTTTTAATCAAGTTATTTATTTTGATTTGTACTTCATCATATTGTTGTTGCTTATAAGCAATATCGTGATGAAGTGCAGCGCCATCAACTTG  >  NC_007793/46188‑46377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑