Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	407,945	C→T	P27P (CCG→CCA)	SAUSA300_RS01890 ←	metal‑dependent hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	407,945	0	C	T	100.0%	76.9 / NA	29	P27P (CCG→CCA)	SAUSA300_RS01890	metal‑dependent hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/28);  total (1/28)

CGTTGCACATAGAAACCATGATCTTTAACCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCCGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATTGTGTCATA  >  NC_007793/407862‑408026                                                                                    |                                                                                  cGTTGCACATAGAAACCATGATCTTTAACCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCg                                                                   <  1:1751844/100‑1 (MQ=255)   ttGCACATAGAAACCGTGATCTTTAACCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGtt                                                                 <  1:4586282/100‑1 (MQ=255)                tcttccgatctAACCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAg                                                    <  1:5646349/90‑1 (MQ=255)                    gATCTTTAACCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGc                                                >  1:532924/1‑100 (MQ=255)                    cgatcttAGCCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGc                                                <  1:3251347/95‑1 (MQ=255)                        tttAACCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGt                                            <  1:716823/100‑1 (MQ=255)                          ttaCCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGttg                                          <  1:4876637/98‑2 (MQ=255)                           tgccGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGttgc                                         <  1:1582309/98‑3 (MQ=255)                                             atcTTCGCCCTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGc                       <  1:4323761/98‑1 (MQ=255)                                             aaCTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGc                       <  1:3988459/100‑1 (MQ=255)                                               tctCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCa                     <  1:2302720/98‑1 (MQ=255)                                               tccgatcTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGccg                     <  1:676904/94‑2 (MQ=255)                                                ttCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCAt                    <  1:899116/100‑1 (MQ=255)                                                ttCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCAt                    <  1:678988/100‑1 (MQ=255)                                                ttCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCAt                    <  1:3534428/100‑1 (MQ=255)                                                 tCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCAtc                   <  1:1810148/100‑2 (MQ=255)                                                  gatcTTTTTCAAACTCACTAGAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATaa                  <  1:1631966/97‑1 (MQ=255)                                                  cGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATaa                  <  1:400411/100‑1 (MQ=255)                                                  cGCCTTTTTCAAACTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATaa                  <  1:447109/100‑1 (MQ=255)                                                            aaaCTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATtgt        <  1:256299/100‑1 (MQ=255)                                                             aaCTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATtgtg       <  1:4058005/100‑1 (MQ=255)                                                               cTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATtgtggc     <  1:2965028/100‑3 (MQ=255)                                                               cTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATTGTGTc     <  1:2309873/100‑1 (MQ=255)                                                               cTCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATTGTGTc     <  1:5776990/100‑1 (MQ=255)                                                                tCACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATTGTGTCa    <  1:1806216/100‑1 (MQ=255)                                                                 cACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATtgtgcctg   <  1:713113/100‑5 (MQ=255)                                                                 cACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATTGTGTcgg   <  1:754510/100‑3 (MQ=255)                                                                 cACTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATTGTGTCAt   <  1:4493503/100‑1 (MQ=255)                                                                  aCTAAAACGTGGTATTTCTGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATTGTGTCATa  <  1:1316523/100‑1 (MQ=255)                                                                                    |                                                                                  CGTTGCACATAGAAACCATGATCTTTAACCGTGAATAAAGTTGAAACTTCGCCTTTTTCAAACTCACTAAAACGTGGTATTTCCGGATCAAATGTATGCGTTAAATCAACCCAAGTTGCTTGTTTTAAAGTATTTAATTGTTGCCATAAAGGATATTGTGTCATA  >  NC_007793/407862‑408026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑