Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	402,447	C→T	Y229Y (TAC→TAT)	SAUSA300_RS01860 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	402,447	0	C	T	100.0%	58.3 / NA	21	Y229Y (TAC→TAT)	SAUSA300_RS01860	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/2);  total (19/2)

TGTAGTAATTGGGCTTTATTCAATTACTACTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTACTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAGAGGAGCATGGATATGACAACATTGTTAAACGTAGATAGTGTGAACAAACAATACAA  >  NC_007793/402351‑402542                                                                                                 |                                                                                                tGTAGTAATTGGGCTTTATTCAATTACTACTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTa                                                                                              <  1:3281392/100‑1 (MQ=255)                  aTTCAATTACTACTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGttt                                                                             >  1:4818675/1‑100 (MQ=255)                     cAATTACTACTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTtta                                                                          >  1:1947674/1‑100 (MQ=255)                         tactacTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTattaa                                                                      >  1:5056493/1‑100 (MQ=255)                         tactacTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTattaa                                                                      >  1:394273/1‑100 (MQ=255)                          actactGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTattaaa                                                                     >  1:4945623/1‑100 (MQ=255)                            tactGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTattaaaat                                                                   >  1:2187023/1‑100 (MQ=255)                             tctGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTattaaaatt                                                                  >  1:4738751/2‑100 (MQ=255)                             actGGAATTAATTAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTAAGTTATTTATTGAAGAGAAGACGTTTTTattaaaatt                                                                  >  1:5550673/1‑100 (MQ=255)                             actGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTattaaaatt                                                                  >  1:2661043/1‑100 (MQ=255)                             actGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTattaaaatt                                                                  >  1:3358006/1‑100 (MQ=255)                             actGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTattaaaatt                                                                  >  1:4664702/1‑100 (MQ=255)                             actGGAATTAATCAAAGCTCTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTattaaaatt                                                                  >  1:4672651/1‑100 (MQ=255)                                         cAAAGCTTTGGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAgagga                                                      >  1:4873169/1‑100 (MQ=255)                                         cAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAagagta                                                      >  1:1129033/1‑98 (MQ=255)                                         cAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAgagga                                                      >  1:4137300/1‑100 (MQ=255)                                                          aCTTATCATTGCTATTTTCATATATAACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAGAGGAGCATGGATATGacaaca                                     >  1:2342290/1‑100 (MQ=255)                                                                                  taACGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAGAGGAGCATGGATATGACAACATTGTTAAACGTAGATAGTGTGaac             >  1:1019076/1‑100 (MQ=255)                                                                                   aaCGCCTTTAGTTATTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAGAGGAGCATGGATATGACAACATTGTTAAACGTAGATAGTGTGaaca            >  1:644005/1‑100 (MQ=255)                                                                                          ttAGTTATTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAGAGGAGCATGGATATGACAACATTGTTAAACGTAGATAGTGTGAacaaacaaca     >  1:1390820/1‑98 (MQ=255)                                                                                             gTTATTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAGAGGAGCATGGATATGACAACATTGTTAAACGTAGATAGTGTGAACAAACAATACaa  <  1:4410173/100‑1 (MQ=255)                                                                                                 |                                                                                                TGTAGTAATTGGGCTTTATTCAATTACTACTGGAATTAATCAAAGCTTTAGTTTGCTACTTATCATTGCTATTTTCATATATAACGCCTTTAGTTACTTATTGAAGAGAAGACGTTTTTATTAAAATTAAACAAAGAGGAGCATGGATATGACAACATTGTTAAACGTAGATAGTGTGAACAAACAATACAA  >  NC_007793/402351‑402542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑