Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	396,520	T→C	I166T (ATA→ACA)	SAUSA300_RS01825 →	Efem/EfeO family lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	396,520	0	T	C	100.0%	18.8 / NA	9	I166T (ATA→ACA)	SAUSA300_RS01825	Efem/EfeO family lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/0);  total (9/0)

ATTGCATGCCAAAGCTGATACATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTT  >  NC_007793/396488‑396616                                 |                                                                                                 aTTGCATGCCAAAGCTGATACATTAGATATCACACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGt                               >  1:1455732/1‑100 (MQ=255)  ctGCATGCCAAAGCTGATACATTAGATATCACACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACCGGTg                              >  1:5742582/2‑100 (MQ=255)   tGCATGCCAAAGCTGATACATTAGATATCACACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTga                             >  1:224304/1‑100 (MQ=255)      aTGCCAAAGCTGATACATTAGATATCACACCAAAATTAATGTTACAAGGTCCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTgaaga                          >  1:5227367/1‑100 (MQ=255)        gCCAAAGCTGATACATTAGATATCACACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTgaagaag                        >  1:3901882/1‑100 (MQ=255)        gCCAAAGCTGATACATTAGATATCACACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTgaagaag                        >  1:5612050/1‑100 (MQ=255)         ccAAAGCTGATACATTAGATATCACACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTgaagaaga                       >  1:830894/1‑100 (MQ=255)                              tCACACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGAttt  >  1:2645305/1‑100 (MQ=255)                              tCACACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGAttt  >  1:5626707/1‑100 (MQ=255)                                 |                                                                                                 ATTGCATGCCAAAGCTGATACATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTT  >  NC_007793/396488‑396616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑