Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	381,188	A→G	S327G (AGC→GGC)	SAUSA300_RS01745 →	lipoate‑‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	381,188	0	A	G	100.0%	144.9 / NA	51	S327G (AGC→GGC)	SAUSA300_RS01745	lipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/2);  total (49/2)

GATATAAAAGATGTGGAAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTAGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAGTGTTTGCTTGCTCTTGAA  >  NC_007793/381092‑381284                                                                                                 |                                                                                                 gATATAAAAGATGTGGAAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCa                                                                                               >  1:3910507/1‑100 (MQ=255)   tataAAAGATGTGGAAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTCGGCaaa                                                                                             >  1:4862565/1‑99 (MQ=255)      aaaaGATGTGGAAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCaagatc                                                                                          >  1:5696527/1‑96 (MQ=255)      aaaaGATGTGGAAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGtt                                                                                          >  1:4489826/1‑100 (MQ=255)       aaaGATGTGGAAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTCGGCaaagatc                                                                                         >  1:3234139/1‑95 (MQ=255)        acgATGTGGAAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTAc                                                                                        >  1:5117127/3‑100 (MQ=255)          caTGTGGAAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACgg                                                                                      >  1:3043148/2‑100 (MQ=255)                    acctcttACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCAtt                                                                            >  1:52697/6‑100 (MQ=255)                     ctgcttACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTa                                                                           >  1:1895191/5‑100 (MQ=255)                     agCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTa                                                                           >  1:5419424/1‑100 (MQ=255)                        aTTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTg                                                                        >  1:4086817/1‑100 (MQ=255)                          tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAgatcggaagag                                                                      >  1:2169000/1‑90 (MQ=255)                          tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGa                                                                      >  1:5479754/1‑100 (MQ=255)                          tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGa                                                                      >  1:4332757/1‑100 (MQ=255)                          tACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAATGGa                                                                      >  1:3068158/1‑100 (MQ=255)                          tACAAGGAAC‑AAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGat                                                                     >  1:458751/1‑100 (MQ=255)                           aCAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGat                                                                     <  1:1569185/100‑1 (MQ=255)                              aGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATg                                                                  <  1:869951/100‑1 (MQ=255)                                 aaCAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGAtt                                                               >  1:3463673/1‑100 (MQ=255)                                       gctGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGt                                                         >  1:1226033/3‑100 (MQ=255)                                       aaTGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGt                                                         >  1:4363177/1‑100 (MQ=255)                                        aTGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGGCATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGtt                                                        >  1:1178490/1‑100 (MQ=255)                                        aTGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGtt                                                        >  1:3718428/1‑100 (MQ=255)                                        aTGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGtt                                                        >  1:1529365/1‑100 (MQ=255)                                         gtgcAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTa                                                       >  1:690476/4‑100 (MQ=255)                                         cgACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTa                                                       >  1:3128213/2‑100 (MQ=255)                                          gACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTaa                                                      >  1:3570067/1‑100 (MQ=255)                                          gACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTaa                                                      >  1:291712/1‑100 (MQ=255)                                          gACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTaa                                                      >  1:4830994/1‑100 (MQ=255)                                          gACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTaa                                                      >  1:2530622/1‑100 (MQ=255)                                          gACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTaa                                                      >  1:4446344/1‑100 (MQ=255)                                          gACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTaa                                                      >  1:1662889/1‑100 (MQ=255)                                          gACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTaa                                                      >  1:34771/1‑100 (MQ=255)                                                        tAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTtat                                        >  1:1084775/1‑100 (MQ=255)                                                         aaCGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTtatg                                       >  1:3991437/1‑100 (MQ=255)                                                         aaCGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTtatg                                       >  1:5036616/1‑100 (MQ=255)                                                          aCGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTtatgt                                      >  1:5093914/1‑100 (MQ=255)                                                                cAGTTAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGcga                                >  1:2981252/1‑98 (MQ=255)                                                                    tAAAGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGcctaaga                            >  1:4930261/1‑94 (MQ=255)                                                                      aaGCAATTAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCAt                          >  1:3620023/1‑100 (MQ=255)                                                                            atAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAg                    >  1:3315989/2‑100 (MQ=255)                                                                             tAGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAgt                   >  1:41461/1‑100 (MQ=255)                                                                              aGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAgtg                  >  1:5334794/1‑100 (MQ=255)                                                                              aGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAgtg                  >  1:4684502/1‑100 (MQ=255)                                                                              aGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAgtg                  >  1:3138331/1‑100 (MQ=255)                                                                              aGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAgtg                  >  1:5680392/1‑100 (MQ=255)                                                                              aGACATCGTTTATTATTTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAgtg                  >  1:609871/1‑100 (MQ=255)                                                                                         attattTTGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAGTGTTTGCTTGctc       >  1:3325390/1‑100 (MQ=255)                                                                                             ctttGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAGTGTTTGCTTGCTCTTGa   >  1:2705892/2‑100 (MQ=255)                                                                                              tttGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAGTGTTTGCTTGCTCTTGaa  >  1:2685759/1‑100 (MQ=255)                                                                                              tttGGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAGTGTTTGCTTGCTCTTGaa  >  1:983098/1‑100 (MQ=255)                                                                                                 |                                                                                                 GATATAAAAGATGTGGAAGAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTAGACATCGTTTATTATTTTAGCAATGTTACGGTAGAAGCATTAGTGGATATGATTTTAAGTTAATATTGTTATTTTATGTATGCTGAATCATTGGAAGTGTTTGCTTGCTCTTGAA  >  NC_007793/381092‑381284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑