Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	376,231	T→G	S200R (AGT→CGT)	SAUSA300_RS01715 ←	NADH‑dependent flavin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	376,231	0	T	G	100.0%	63.8 / NA	25	S200R (AGT→CGT)	SAUSA300_RS01715	NADH‑dependent flavin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (3/22);  total (3/22)

TCTGGCGTTGCTCGAAATCCTAAAATAAAATTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACTATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTGCAATCGAAATT  >  NC_007793/376134‑376325                                                                                                  |                                                                                               tctGGCGTTGCTCGAAATCCTAAAATAAAATTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGAt                                                                                              >  1:5337175/1‑100 (MQ=255)                      aaaataaaatTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGt                                                                         <  1:5078110/100‑1 (MQ=255)                        aataaaatTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCCAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTac                                                                       <  1:484536/100‑2 (MQ=255)                           cgatctGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCGTAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATgg                                                                    <  1:1428333/95‑1 (MQ=255)                           aaaatTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATgg                                                                    <  1:5643646/100‑1 (MQ=255)                            aaatTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGt                                                                   <  1:126975/100‑1 (MQ=255)                             aatTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATgggc                                                                  <  1:1514107/100‑3 (MQ=255)                              atTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCt                                                                 <  1:5414483/100‑1 (MQ=255)                               ttGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTg                                                                <  1:690400/100‑1 (MQ=255)                                tGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTgg                                                               <  1:2388834/100‑2 (MQ=255)                                tGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGt                                                               <  1:1532224/100‑1 (MQ=255)                                 gTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGtc                                                              <  1:4989993/100‑2 (MQ=255)                                      gTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGttt                                                         <  1:4466008/100‑1 (MQ=255)                                       tGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGtttt                                                        <  1:2576097/100‑2 (MQ=255)                                                   catcaCTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGtt                                            >  1:4630907/2‑100 (MQ=255)                                                    ttccgatcttGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTg                                           <  1:2595257/94‑1 (MQ=255)                                                    atcaCTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTg                                           <  1:918361/100‑1 (MQ=255)                                                    atcaCTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTg                                           <  1:3678973/100‑1 (MQ=255)                                                      caCTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTgag                                         <  1:1299768/100‑1 (MQ=255)                                                             tGTACCGCACGCATAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATg                                  >  1:1100967/1‑100 (MQ=255)                                                                         aTAACTTCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCa                      <  1:4124350/100‑1 (MQ=255)                                                                              ttCTAAACATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCaaggaatca                 <  1:315829/100‑9 (MQ=255)                                                                                   aaCATAATCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTGc            <  1:2239818/100‑1 (MQ=255)                                                                                          tCTTGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAGCGTTGTGCAATCGaa     <  1:3676036/100‑1 (MQ=255)                                                                                             tGCACGATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTGCAATCGAAAtt  <  1:2864920/100‑1 (MQ=255)                                                                                                  |                                                                                               TCTGGCGTTGCTCGAAATCCTAAAATAAAATTGTCAGGTGCTTCTTTATCAATCACTTCTTGTACCGCACGCATAACTTCTAAACATAATCTTGCACTATTTTTTAATGAGTCGGCACCGTAATGGTCTGTACGTTTATTCGAAAAAGTTGAGAAAAATGTTTGAATCAGCAAACGTTGTGCAATCGAAATT  >  NC_007793/376134‑376325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑