Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	374,582	A→G	intergenic (+95/+147)	SAUSA300_RS01705 → / ← SAUSA300_RS01710	lipase/alpha/beta hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	374,582	0	A	G	100.0%	60.8 / NA	23	intergenic (+95/+147)	SAUSA300_RS01705/SAUSA300_RS01710	lipase/alpha/beta hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/4);  total (19/4)

CATCTTCTGAATTTAATATGCTATGTAAATCGTGCTGTTATCACGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTAAAGTGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGACAGGCGTATCTGTTTATGAGCTTAATAAATTGT  >  NC_007793/374491‑374658                                                                                            |                                                                             cATCTTCTGAATTTAATATGCTATGTAAATCGTGCTGTTATCACGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATTGTGAAgatcgg                                                                      >  1:4628039/1‑95 (MQ=255)      tctGAATTTAATATGCTATGTAAATCGTGCTGTTATCACGGCACATCATATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAg                                                                 >  1:565703/1‑100 (MQ=255)      tctGAATTTAATATGCTATGTAAATCGTGCTGTTATCACGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAg                                                                 >  1:521578/1‑100 (MQ=255)               aaTATGCTATGTAAATCGTGCTGTTATCACGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGtct                                                        <  1:1709658/100‑1 (MQ=255)                atatGCTATGTAAATCGTGCTGTTATCACGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGtctc                                                       <  1:1800608/100‑1 (MQ=255)                   tGCTATGTAAATCGTGCTGTTATCACGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGTCTCAtt                                                    <  1:4874754/100‑1 (MQ=255)                   atcTATGTAAATCGTGCTGTTATCACGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGTCTCAtt                                                    >  1:2651875/3‑100 (MQ=255)                    gCTATGTAAATCGTGCTGTTATCACGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGccagatcggaag                                                   >  1:4174692/1‑90 (MQ=255)                       aTGTAAATCGTGCTGTTATCACGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGTCTCATTAGCg                                                >  1:304600/1‑100 (MQ=255)                                     gTTATCACGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGAc                                  >  1:192721/1‑100 (MQ=255)                                        aTCACGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGACAgg                               >  1:2824711/1‑100 (MQ=255)                                        aTCACGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGGGAAGTGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGACAgg                               >  1:1227506/1‑100 (MQ=255)                                           aCGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGACAGGCGt                            >  1:5235484/1‑100 (MQ=255)                                            cGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGACAGGCGTa                           >  1:3000344/1‑100 (MQ=255)                                            cGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGACAGGCGTa                           >  1:3187571/1‑100 (MQ=255)                                             ggCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGACAGGCGTAt                          >  1:1120693/1‑100 (MQ=255)                                             ctcacaTCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGACAGGCGTAt                          >  1:5287813/3‑100 (MQ=255)                                               cacaTCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGACAGGCGTATCt                        >  1:1650998/1‑100 (MQ=255)                                                acaTCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGACAGGCGTATCTg                       >  1:1466331/1‑100 (MQ=255)                                                   tCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGACAGGCGTATCTGttt                    <  1:3195414/100‑1 (MQ=255)                                                           tgctAGCATCACAGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGACAGGCGTATCTGTTTATGAGCtt            >  1:2092915/4‑100 (MQ=255)                                                              aggCATCACAGTGTTGAATTTAAAAATAGTGAAATGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGACAGGCGTATCTGTTTATGAGCTtaat         >  1:1418744/3‑100 (MQ=255)                                                                     acaGTGTTGAATTTAAAAATAGTGAAGTGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGACAGGCGTATCTGTTTATGAGCTTAATAAATTGt  >  1:421079/1‑100 (MQ=255)                                                                                            |                                                                             CATCTTCTGAATTTAATATGCTATGTAAATCGTGCTGTTATCACGGCACATCAGATATAAGTAGCATCACAGTGTTGAATTTAAAAATAGTAAAGTGAAATAAAGCGCCTGTCTCATTAGCGAAAACTAAAGGGACAGGCGTATCTGTTTATGAGCTTAATAAATTGT  >  NC_007793/374491‑374658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑