D66 :: BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	372,740	T→C	M109T (ATG→ACG)	SAUSA300_RS01705 →	lipase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	372,740	0	T	C	99.5%	988.5 / ‑4.9	365	M109T (ATG→ACG)	SAUSA300_RS01705	lipase
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (354/9); total (356/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 365 total aligned reads displayed.

TAAAGAGAATGGTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAATGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGTTCTAAACAGTCACACCAAAATAATGCGACTAATAATACTGAACGTCAAAATGA  >  NC_007793/372645‑372836

 aaaGAGAATGGTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCaaa                                                                                             >  1:4029998/1‑100 (MQ=255)

   actgaATGGTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCaaaga                                                                                           >  1:292852/4‑98 (MQ=255)

      gaATGGTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCaatagatc                                                                                        >  1:1232263/1‑94 (MQ=255)

      acaTGGTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGAt                                                                                        >  1:662044/3‑100 (MQ=255)

      acaTGGTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGAt                                                                                        >  1:720611/3‑100 (MQ=255)

      aaaTGGTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGAt                                                                                        >  1:3466131/2‑100 (MQ=255)

       aaTGGTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATa                                                                                       >  1:1417526/1‑100 (MQ=255)

        ctGGTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATaa                                                                                      >  1:4403047/2‑100 (MQ=255)

        aTGGTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCGAAATGATaa                                                                                      >  1:1106012/1‑100 (MQ=255)

        aTGGTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATaa                                                                                      >  1:5586015/1‑100 (MQ=255)

        aTGGTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATaa                                                                                      >  1:2079200/1‑100 (MQ=255)

        aTGGTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATaa                                                                                      >  1:3425014/1‑100 (MQ=255)

          ggTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCaaaagatcggaa                                                                                    >  1:4237123/1‑92 (MQ=255)

          ggTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATc                                                                                    >  1:4705567/1‑100 (MQ=255)

          ggTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATc                                                                                    >  1:3064649/1‑100 (MQ=255)

          ggTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATc                                                                                    >  1:79271/1‑100 (MQ=255)

          ggTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATc                                                                                    >  1:1204115/1‑100 (MQ=255)

                aGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATagatcggaag                                                                              >  1:873243/1‑91 (MQ=255)

                aGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAgatc                                                                              >  1:4190937/1‑97 (MQ=255)

                 gTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTaaa                                                                             >  1:2989793/1‑100 (MQ=255)

                 ctagAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTaaa                                                                             >  1:1335007/4‑100 (MQ=255)

                 agggAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTaaa                                                                             >  1:1161055/3‑100 (MQ=255)

                  tGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTACAACAATCAGAACGCAAAATGATAATCAAGTAAAg                                                                            >  1:2542783/1‑100 (MQ=255)

                  aggAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAg                                                                            >  1:4881924/2‑100 (MQ=255)

                   ggAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAgcagcaa                                                                           >  1:2149047/1‑94 (MQ=255)

                   ggAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTaacca                                                                           >  1:1750986/1‑97 (MQ=255)

                   ggAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGc                                                                           >  1:417138/1‑100 (MQ=255)

                   ggAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGc                                                                           >  1:5721108/1‑100 (MQ=255)

                   ggAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGc                                                                           >  1:2200126/1‑100 (MQ=255)

                   ggAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGc                                                                           >  1:4339314/1‑100 (MQ=255)

                   gcaaCAGTGACAGAAGGTAAGGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAgt                                                                           >  1:319851/3‑99 (MQ=255)

                    taaCAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCa                                                                          >  1:1051463/2‑100 (MQ=255)

                     aaCAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCaa                                                                         >  1:182293/1‑100 (MQ=255)

                      gctgTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAg                                                                        >  1:5037837/4‑100 (MQ=255)

                      aCAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCGAgaagatcgga                                                                        >  1:707621/1‑91 (MQ=255)

                      aCAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAg                                                                        >  1:1686127/1‑100 (MQ=255)

                      aCAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAg                                                                        >  1:2359883/1‑100 (MQ=255)

                      aCAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAg                                                                        >  1:4058107/1‑100 (MQ=255)

                       taGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGa                                                                       >  1:2112619/2‑100 (MQ=255)

                       cAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTACAGCAAGa                                                                       >  1:4532388/1‑100 (MQ=255)

                       cAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGa                                                                       >  1:2294622/1‑100 (MQ=255)

                       cAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGa                                                                       >  1:2965072/1‑100 (MQ=255)

                       cAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGa                                                                       >  1:1904164/1‑100 (MQ=255)

                       aaGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGa                                                                       >  1:2515361/2‑100 (MQ=255)

                        tgTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGAt                                                                      >  1:1639456/2‑100 (MQ=255)

                        tgTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGAt                                                                      >  1:3815564/2‑100 (MQ=255)

                        tgTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGAt                                                                      >  1:84929/2‑100 (MQ=255)

                        ggTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAACCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGAt                                                                      >  1:3996880/2‑100 (MQ=255)

                        cctGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTCAAGCAAGAt                                                                      >  1:2438980/3‑100 (MQ=255)

                        aGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACTCAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAGAGCAAGAt                                                                      >  1:2646946/1‑100 (MQ=255)

                        aGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTGAAGCAAGAt                                                                      >  1:3604942/1‑100 (MQ=255)

                        aGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGag                                                                      >  1:2269699/1‑99 (MQ=255)

                         gTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGAtt                                                                     >  1:1864246/1‑100 (MQ=255)

                         gTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGAtt                                                                     >  1:1550158/1‑100 (MQ=255)

                         gTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGAtc                                                                     >  1:5220362/1‑99 (MQ=255)

                         gTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGAtc                                                                     >  1:4379094/1‑99 (MQ=255)

                         gTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGAtc                                                                     >  1:275710/1‑99 (MQ=255)

                         ctGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGAtt                                                                     >  1:1711543/2‑100 (MQ=255)

                          tGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTGAAGCAAGAtcg                                                                    >  1:5545087/1‑98 (MQ=255)

                          tGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTc                                                                    >  1:5260595/1‑100 (MQ=255)

                          tGACAGAAGGTAAAGATACGCTTCAATAATCGAAGAATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTGAAGCAAGAtcg                                                                    >  1:5368259/1‑98 (MQ=255)

                          tGACAGAAGGAAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGAtcg                                                                    >  1:903363/1‑98 (MQ=255)

                           gACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTGAAGCAAGAtccg                                                                   >  1:3665324/1‑97 (MQ=255)

                           gACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAGGCAAGAtcgg                                                                   >  1:1271484/1‑97 (MQ=255)

                           gACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAgatcggaaga                                                                   >  1:5654012/1‑91 (MQ=255)

                           gACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGAtcgg                                                                   >  1:4847816/1‑97 (MQ=255)

                           gACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCt                                                                   >  1:3128640/1‑100 (MQ=255)

                           gACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCt                                                                   >  1:3333987/1‑100 (MQ=255)

                           gACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCt                                                                   >  1:3587196/1‑100 (MQ=255)

                           gACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCt                                                                   >  1:4793054/1‑100 (MQ=255)

                           gACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCt                                                                   >  1:4666933/1‑100 (MQ=255)

                           gACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCt                                                                   >  1:4453320/1‑100 (MQ=255)

                           gACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCt                                                                   >  1:3904107/1‑100 (MQ=255)

                           gACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCt                                                                   >  1:3981375/1‑100 (MQ=255)

                           gACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGT‑AAGCAAGAtcgga                                                                  >  1:449165/1‑96 (MQ=255)

                           gACAGAAGGCAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTATAGCAAGAtcgg                                                                   >  1:4134018/1‑97 (MQ=255)

                            aCAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTg                                                                  >  1:3293375/1‑100 (MQ=255)

                              ccaaGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAGTAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGAtcggaag                                                                >  1:5313014/3‑94 (MQ=255)

                              ataaGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAGTAGTAAAACAATCAGAACGCAAAATGATAATCAAGTACAGCAAGAtcggaag                                                                >  1:1463460/3‑94 (MQ=255)

                                caGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGACAATCAAGTAAAGCAAGAtcggaagag                                                              >  1:5304789/2‑92 (MQ=255)

                                 aGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGa                                                             >  1:343125/1‑100 (MQ=255)

                                  ggTAAGGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAGAGCAAGAtcggaagagca                                                            >  1:3027500/1‑90 (MQ=255)

                                  ggTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAGCGAc                                                            >  1:2881121/1‑100 (MQ=255)

                                       gtgcaCGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGt                                                       >  1:4547582/5‑100 (MQ=255)

                                           aCGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAgatcgga                                                   >  1:1389088/1‑94 (MQ=255)

                                              cttccgatctcGAAGCATCAATCAACACAAAATAGTAAAACAAACAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGTTCTAAAc                                                <  1:5192262/91‑1 (MQ=255)

                                                  aataatCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAGGATTCTGAACGACAAGGTTCTAAACAGTc                                            <  1:4815192/100‑1 (MQ=255)

                                                        cgtgtgctcaatcaaCACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGTTCTAAACAGTCACACCa                                      >  1:5443217/8‑100 (MQ=255)

                                                        cGAAGCATCAATCAACACAAAATAGTAAATCAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGCCAAGGTTCTAAACAGTCACACCa                                      >  1:1975284/1‑100 (MQ=255)

                                                              aagcacacacacAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGTTCTAAACAGTCACACCAAaataa                                >  1:5119495/9‑100 (MQ=255)

                                                                   tcaaCACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGTTCTAAACAGTCACACCAAAATAATGCGa                           >  1:2841951/1‑100 (MQ=255)

                                                                            aaTAGTAAAACAATCAGAGCGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGTTCTAAACAGTCACACCAAAATAATGCGACtaataaga                  >  1:2053590/1‑98 (MQ=255)

                                                                                  caaaCAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGTTCTAAACAGTCACACCAAAATAATGCGACTAATAATACTGAAc            >  1:5688188/2‑100 (MQ=255)

                                                                                  aaaaCAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGTTCTAAACAGTCACACCAAAATAATGCGACTAATAATACTGAAc            >  1:4763683/1‑100 (MQ=255)

                                                                                    atcAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGTTCTAAACAGTCACACCAAAATAATGCGACTAATAATACTGAACGt          >  1:1017517/3‑100 (MQ=255)

                                                                                    aaCAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGTTCTAAACAGTCACACCAAAATAATGCGACTAATAATACTGAACGt          >  1:3414080/1‑100 (MQ=255)

                                                                                     aCAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGTTCTAAACAGTCACACCAAAATAATGCGACTAATAATACTGAACGTc         >  1:3783899/1‑100 (MQ=255)

                                                                                          cAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGTTCTAAACAGTCACACCAAAATAATGCGACTAATAATACTGAACGTCAAAAt    >  1:3746991/1‑100 (MQ=255)

                                                                                            gAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGTTCTAAACAGTCACACCAAAATAATGCGACTAATAATACTGAACGTCAAAATGa  >  1:4969387/1‑100 (MQ=255)

                                                                                            gAACGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGTTCTAAACAGTCACACCAAAATAATGCGACTAATAATACTGAACGTCAAAATGa  >  1:40873/1‑100 (MQ=255)

TAAAGAGAATGGTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCAACACAAAATAGTAAAACAATCAGAATGCAAAATGATAATCAAGTAAAGCAAGATTCTGAACGACAAGGTTCTAAACAGTCACACCAAAATAATGCGACTAATAATACTGAACGTCAAAATGA  >  NC_007793/372645‑372836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`