Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	349,857	G→A	intergenic (+304/‑282)	SAUSA300_RS01585 → / → SAUSA300_RS01590	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	349,857	0	G	A	100.0%	54.8 / NA	22	intergenic (+304/‑282)	SAUSA300_RS01585/SAUSA300_RS01590	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/0);  total (22/0)

ATCAAGATTATTATAATTGTATTACATATAGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTGCTATAATTATAAATAGTTAAATAAATAGAAAGACAAAATAATGAGTAGGAGTTTTAGG  >  NC_007793/349767‑349915                                                                                           |                                                           aTCAAGATTATTATAATTGTATTACATATAGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATta                                                   >  1:4446442/1‑100 (MQ=255)  gcAAGATTATTATAATTGTATTACATATAGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATtat                                                  >  1:4745019/2‑100 (MQ=255)   cAAGATTATTATAATTGTATTACATATAGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATtata                                                 >  1:5279702/1‑100 (MQ=255)               ccttGTATTACATATAGGTGTTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATCAGTATCTTTTTTACTATAATTATAAATAGTtaaata                                     >  1:42555/3‑100 (MQ=14)                       tACATATAGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGaaa                             >  1:906495/1‑100 (MQ=255)                       tACATATAGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGaaa                             >  1:5736212/1‑100 (MQ=255)                       tACATATAGGTGCTGGTAAATTTTATTTACGAAATGGC‑AAAAAGTATATACTATTAATATC‑TTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGa                           >  1:5423765/1‑100 (MQ=17)                        aCATATAGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAg                            >  1:3651689/1‑100 (MQ=255)                            gtaGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACaa                        >  1:5738957/2‑100 (MQ=255)                             taGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACaaa                       >  1:3502109/1‑100 (MQ=255)                             taGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACaaa                       >  1:4748052/1‑100 (MQ=255)                             taGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACaaa                       >  1:5650351/1‑100 (MQ=255)                             ctggTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACaaa                       >  1:1502767/3‑100 (MQ=255)                             caGGTGCTGGTAAATTTTATTTACGAAATGGC‑AAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACaaaa                      >  1:3868974/2‑100 (MQ=255)                             aaGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACaaa                       >  1:4126308/2‑100 (MQ=255)                              aGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACaaaa                      >  1:1900472/1‑100 (MQ=255)                              aGGTGCTGGTAAATTTTATTTACGAAACGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACaaaa                      >  1:1292518/1‑100 (MQ=255)                               tgTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACAAaat                     >  1:4606975/2‑100 (MQ=255)                                     ggTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACAAAATAATGAg               >  1:2657160/1‑100 (MQ=255)                                                tttaCGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACAAAATAATGAGtagtagatcgg    >  1:1846364/1‑95 (MQ=14)                                                tataCGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACAAAATAATGAGTAGGAgatcgg    >  1:2955824/3‑95 (MQ=255)                                                  taCGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTACTATAATTATAAATAGTTAAATAAATAGAAAGACAAAATAATGAGCAGGAgatcggaa  >  1:3172485/1‑93 (MQ=14)                                                                                           |                                                           ATCAAGATTATTATAATTGTATTACATATAGGTGCTGGTAAATTTTATTTACGAAATGGCAAAAAAGTATATACTATTAATATCTTTTTTGCTATAATTATAAATAGTTAAATAAATAGAAAGACAAAATAATGAGTAGGAGTTTTAGG  >  NC_007793/349767‑349915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑