| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 348,143 | T→A | pseudogene (481/496 nt) | SAUSA300_RS01575 → | QueA protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 348,143 | 0 | T | A | 88.9% | 13.8 / ‑3.2 | 9 | pseudogene (481/496 nt) | SAUSA300_RS01575 | QueA protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (7/1); total (8/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01 | |||||||||||
TATAAGTATAGAAAATTTGGAATTTTACCAGAAACGGAATATGAAATTAATAAAGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGATGAAGCTGAACTATAGGGGCGATAATATGACTTTCGAAGAAAAATTAAGTGAAATGTATAGCGAGATTGCGAATAAGATTATTAGCAT > NC_007793/348054‑348230 | tataAGTATAGAAAATTTGGAATTTTCCCAGAAACGGAATATGAAATTAATAAAGTTAAAGAAATCGAGCAATATATTAAAGAGCTAGAtcggaagagca > 1:1155194/1‑90 (MQ=11) ataAGTATAGAAAATTTGGAATTTTACCAGAAACGGAATATGAAATTTTTAACGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAAGCTGAACt > 1:2708045/1‑100 (MQ=11) ataGAAAATTTGGAATTTTACCAGAAACGGAATATGAAATTAATAAAGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAAGCTGAACTATAggg < 1:4746665/100‑1 (MQ=25) tttACCCGAAACGGAATATGAAATTAATAAAGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAAGCTGAACTATATTTTCGATAATATGACttt > 1:3926085/1‑100 (MQ=14) ccAGAAACGGAATATGAAATTAATAAAGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAAGCTGAACTATAGGGGCGATAATATGACTTTCgaa > 1:3417324/1‑100 (MQ=255) cAGAAACGGAATATGAAATTAATAAAGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAAGCTGAACTATAGGGGCGATAATATGACTTTCgaag > 1:3000987/1‑100 (MQ=255) aaaaCGGAATATGAAATTAATAAAGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAAGCTGAACTATAGGGGCGATAATATGACTTTCgaagaa > 1:3890408/2‑100 (MQ=255) atGAAATTAATAAAGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAAGCTGAACTATAGGGGCGATAATATGACTTTCGAAGAAAAATTAAGTg > 1:1962271/1‑100 (MQ=255) atatTAAAGAGCAAGAAGAAGCTGAACTATAGGGGCGATAATATGACTTTCGAAGAAAAATTAAGTGAAATGTATAGCGAGATTGCGAATAAGAttatta > 1:5317163/1‑100 (MQ=255) tAAAGAGCAAGATGAAGCTGAACTATAGGGGCGATAATATGACTTTCGAAGAAAAATTAAGTGAAATGTATAGCGAGATTGCGAATAAGATTATTAGCAt > 1:2767220/1‑100 (MQ=255) | TATAAGTATAGAAAATTTGGAATTTTACCAGAAACGGAATATGAAATTAATAAAGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGATGAAGCTGAACTATAGGGGCGATAATATGACTTTCGAAGAAAAATTAAGTGAAATGTATAGCGAGATTGCGAATAAGATTATTAGCAT > NC_007793/348054‑348230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |