Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	345,030	A→G	Y106C (TAT→TGT)	SAUSA300_RS01550 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	345,030	0	A	G	100.0%	72.9 / NA	27	Y106C (TAT→TGT)	SAUSA300_RS01550	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/12);  total (15/12)

TGCACAGAAATATAAAAAAATATATGCTCGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTATTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCTATTTATTATTAGTAATTGGCGG  >  NC_007793/344935‑345126                                                                                                |                                                                                                 tGCACAGAAATATAAAAAAATATATGCTCGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGtttt                                                                                              >  1:4273125/1‑100 (MQ=255) tGCACAGAAATATAAAAAAATATATGCTCGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGtttt                                                                                              >  1:2418488/1‑100 (MQ=255)  gCACAGAAATATAAAAAAATATATGCTCGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTg                                                                                             >  1:348082/1‑100 (MQ=255)   cacaGAAATATAAAAAAATATATGCTCGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTgg                                                                                            <  1:4024994/100‑2 (MQ=255)   cacaGAAATATAAAAAAATATATGCTCGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGc                                                                                            <  1:1389206/100‑1 (MQ=255)   cacaGAAATATAAAAAAATATATGCTCGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGc                                                                                            <  1:961124/100‑1 (MQ=255)       gAAATATAAAAAAATATATGCTCGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCt                                                                                        <  1:3560898/100‑1 (MQ=255)           tcttccgatctatatGTTCGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTAc                                                                                    <  1:4462609/90‑1 (MQ=255)                                          taaacggtgtgTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGtt                                                     >  1:230619/8‑100 (MQ=255)                                           tataAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTg                                                    <  1:2136529/100‑1 (MQ=255)                                              aaaTGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATg                                                 <  1:354618/100‑1 (MQ=255)                                              aaaTGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATg                                                 <  1:3432504/100‑1 (MQ=255)                                               aaTGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATgg                                                <  1:1168723/100‑1 (MQ=255)                                               aaTGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATgg                                                >  1:4049268/1‑100 (MQ=255)                                                aTGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATggg                                               <  1:2740689/100‑1 (MQ=255)                                                  gtgtTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGtt                                             <  1:1434542/100‑1 (MQ=255)                                                    gtTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCg                                           <  1:4122853/100‑1 (MQ=255)                                                           actaTCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCaa                                    >  1:2521858/1‑100 (MQ=255)                                                            ctaTCACTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAAt                                   >  1:3730017/1‑100 (MQ=255)                                                              aTCACTGTAGTATTTGCACTGTGTAATCTTTACTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCa                                 >  1:2657504/1‑100 (MQ=255)                                                                  cTGTAGTATTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAAt                             >  1:3144599/1‑100 (MQ=255)                                                                    gtagtaTTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTa                           >  1:2785055/1‑100 (MQ=255)                                                                    atagtaTTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTa                           >  1:2630381/2‑100 (MQ=255)                                                                         aTTTGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCtat                      >  1:3112513/1‑100 (MQ=255)                                                                          cttGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCtatt                     >  1:1873963/2‑100 (MQ=255)                                                                            tGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCtattta                   >  1:5441872/1‑100 (MQ=255)                                                                            tGCACTGTGTAATCTTTATTGTTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCtattta                   >  1:622868/1‑100 (MQ=255)                                                                                             attgttTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCTATTTATTATTAGTAATTGGCgg  <  1:3927000/100‑1 (MQ=255)                                                                                             attgttTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCTATTTATTATTAGTAATTGGCgg  <  1:2604818/100‑1 (MQ=255)                                                                                             attgttTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCTATTTATTATTAGTAATTGGCgg  <  1:5490202/100‑1 (MQ=255)                                                                                                |                                                                                                 TGCACAGAAATATAAAAAAATATATGCTCGATTTTTAGTTGTTATAAATGTGTTAACTACTATCACTGTAGTATTTGCACTGTGTAATCTTTATTATTTTGCAGCTGTACAAAACCATTATGATTTATTTAATTATTGGTTGATGGGTTCGATTTCAATCATAATTAGCTATTTATTATTAGTAATTGGCGG  >  NC_007793/344935‑345126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑