| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 328,214 | A→G | T84T (ACA→ACG) | SAUSA300_RS01475 → | membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 328,214 | 0 | A | G | 93.8% | 34.3 / ‑4.2 | 16 | T84T (ACA→ACG) | SAUSA300_RS01475 | membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (12/3); total (12/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01 | |||||||||||
TTATGGCCTTTACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACACTTAACGTTATTGTGGGATATACTTTGCCATTGCTTTTTGTATCTATTTACGTTTTTGGTGTAGTCGGTTTTGGATTTGATGTTTTCAATTATTGT > NC_007793/328117‑328310 | gtATGGCCTTTACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCt > 1:5782671/2‑100 (MQ=255) tATGGCCTTTACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCtt > 1:1405918/1‑100 (MQ=255) tATGGCCTTTACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCtt > 1:280596/1‑100 (MQ=255) ggCCTTTACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAAc > 1:1831868/1‑100 (MQ=255) ggCCTTTACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAAc > 1:2839500/1‑100 (MQ=255) ttACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTAt > 1:5486736/1‑100 (MQ=255) cTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTATTGTggg < 1:256059/100‑1 (MQ=255) agTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGATATACTTTGCCATTGCt > 1:163512/2‑100 (MQ=255) gTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGATATACTTTGCCATTGCtt > 1:3935570/1‑100 (MQ=255) gTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGATATACTTTGCCATTGCtt > 1:5017909/1‑100 (MQ=255) atacatacaAGAGAAAAATGAAAATTACAAAGTTGACAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGATATACTTTGCCATTGCtt > 1:5410697/2‑100 (MQ=255) acatacaAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGATATACTTTGCCATTGCtttt > 1:1029728/1‑100 (MQ=255) aaaaTTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGATATACTTTGCCATTGCTTTTTGTATCTATTTACGtttg < 1:4279186/100‑2 (MQ=255) aCAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGATATACTTTGCCATTGCTTTTTGTATCTATTTACGTTTTTGgtgt > 1:4619093/1‑100 (MQ=255) aaaaaaGATATGTGGTTAGGTATCTCACACTTAACGTTATTGTGGGATATACTTTGCCATTGCTTTTTGTATCTATTTACGTTTTTGGTGTAGTCGGttt < 1:5508931/100‑1 (MQ=255) gATATGTGGTTAGATATCTCACGCTTAACGTTATTGTGGGATATACTTTGCCATTGCTTTTTGTATCTATTTACGTTTTTGGTGTAGTCGGTTTTGGAtt < 1:3634901/100‑1 (MQ=255) cacgCTTAACGTTATTGTGGGATATACTTTGCCATTGCTTTTTGTATCTATTTACGTTTTTGGTGTAGTCGGTTTTGGATTTGATGTTTTCAATTATTGt > 1:5443905/1‑100 (MQ=255) | TTATGGCCTTTACAGCTGTAATTTTGATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTTAGATATCTCACACTTAACGTTATTGTGGGATATACTTTGCCATTGCTTTTTGTATCTATTTACGTTTTTGGTGTAGTCGGTTTTGGATTTGATGTTTTCAATTATTGT > NC_007793/328117‑328310 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |