Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	316,573	T→G	L56F (TTA→TTC)	SAUSA300_RS01415 ←	ribose transporter RbsU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	316,573	0	T	G	100.0%	35.9 / NA	16	L56F (TTA→TTC)	SAUSA300_RS01415	ribose transporter RbsU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/10);  total (6/10)

ACGCTTTAAATGTAATGATTTGTCCGAATCCCCATCCTGCACCTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGTAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGATGTACAGGTTTACCAC  >  NC_007793/316494‑316661                                                                                |                                                                                         aCGCTTTAAATGTAATGATTTGTCCGAATCCCCATCCTGCACCTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTag                                                                      <  1:1549161/100‑2 (MQ=255)                                ccATCCTGCACCTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGGAAGCCTCTTGATGTGACTCCGGCTAATATAATAGCGAAGATTAACGTACCt                                       <  1:2049702/100‑1 (MQ=255)                                ccATCCTGCACCTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCt                                       <  1:4095352/100‑1 (MQ=255)                                 cATCCTGCACCTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTa                                      >  1:5230044/1‑100 (MQ=255)                                    ccTGCACCTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAg                                   >  1:287880/1‑100 (MQ=255)                                       gCACCTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAg                                >  1:5353974/1‑100 (MQ=255)                                        cACCTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGc                               <  1:4485554/100‑1 (MQ=255)                                           cTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCAcc                            <  1:1300671/100‑1 (MQ=255)                                                     ggCGAATAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGa                  >  1:1590095/1‑100 (MQ=255)                                                          aTAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGATGTAc             <  1:1002333/100‑1 (MQ=255)                                                          aTAGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGATGTAc             <  1:5413655/100‑1 (MQ=255)                                                            aGCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGATGTACAg           <  1:1390111/100‑1 (MQ=255)                                                             gCAAATTGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGATGTACAgc          <  1:2311811/100‑2 (MQ=255)                                                                   tGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGATGTACAGGTTTacc    <  1:5390710/100‑1 (MQ=255)                                                                   tGGTTCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGATGTACAGGTTTacc    >  1:637167/1‑100 (MQ=255)                                                                     tttCCAGTAGGGAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGATGTACAGGTTTaccac  >  1:5472679/2‑100 (MQ=255)                                                                                |                                                                                         ACGCTTTAAATGTAATGATTTGTCCGAATCCCCATCCTGCACCTGATAATAAGGCGAATAGCAAATTGGTTCCAGTAGGTAAGCCACTTGATGTGACTACGGCTAATATAATAGCGAAGATTAACGTACCTACAGTAGCACCGATAATTTGATGTACAGGTTTACCAC  >  NC_007793/316494‑316661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑