Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	315,829	T→C	I6V (ATT→GTT)	SAUSA300_RS01410 ←	D‑ribose pyranase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	315,829	0	T	C	100.0%	16.5 / NA	8	I6V (ATT→GTT)	SAUSA300_RS01410	D‑ribose pyranase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/8);  total (0/8)

TGATCATTTGGAATTGGCATGCCAGCGTCATTAATCGTTAATAAATCAAAATGACCAATTGTCGCGATTGCTTTTGAAATATGTTCATTTAAAATAGCTGATTTTTTCATGACATCTACACTCCTTATTTTATAAATACTGTAACAGAAGCGG  >  NC_007793/315735‑315887                                                                                               |                                                           tgatCATTTGGAATTGGCATGCCAGCGTCATTAATCGTTAATAAATCAAAATGACCAATTGTCGCGATTGCTTTTGAAATATGTTCATTTAAAACAGCTg                                                       <  1:2215971/100‑1 (MQ=255)            aaTTGGCATGCCAGCGTCATTAATCGTTAATAAATCAAAATGACCAATTGTCGCGATTGCTTTTGAAATATGTTCATTTAAAACAGCTGATTTTTTCATg                                            <  1:3939680/100‑1 (MQ=255)                      ccAGCGTCATTAATCGTTAATAAATCAAAATGACCAATTGTCGCGATTGCTTTTGAAATATGTTCATTTAAAACAGCTGATTTTTTCATGACATCTacta                                  <  1:869975/100‑3 (MQ=255)                       cAGCGTCATTAATCGTTAATAAATCAAAATGACCAATTGTCGCGATTGCTTTTGAAATATGTTCATTTAAAACAGCTAATTTTTTCATGACATCTaccac                                 <  1:4548133/100‑4 (MQ=255)                            tCATTAATCGTTAATAAATCAAAATGACCAATTGTCGCGATTGCTTTTGAAATATGTTCATTTAAAACAGCTGATTTTTTCATGACATCTACACTCCttg                            <  1:1275060/100‑2 (MQ=255)                                                 aaaTGCCCAATTGTCGCGATTGCATTTGAAATATGTTCATTTAAAACAGGCGATTTTTTCATGACATCTACACTCCTTATTTTATAAATACTGTAACAGa       <  1:1857477/100‑1 (MQ=255)                                                      gatctttGTCGCGATTGCTTTTGAAATATGTTCATTTAAAACAGCTGATTTTTTCATGACATCTACACTCCTTATTTTATAAATACTGTAACAGAAGCgg  <  1:4298414/95‑1 (MQ=255)                                                      ccgatctGTCTCGATTGCTTTTGAAATATGTTCATTTAAAACAGCTGATTTTTTCATGACATCTACACTCCTTATTTTATAAATACTGTAACAGAAGCgg  <  1:4006726/94‑1 (MQ=255)                                                                                               |                                                           TGATCATTTGGAATTGGCATGCCAGCGTCATTAATCGTTAATAAATCAAAATGACCAATTGTCGCGATTGCTTTTGAAATATGTTCATTTAAAATAGCTGATTTTTTCATGACATCTACACTCCTTATTTTATAAATACTGTAACAGAAGCGG  >  NC_007793/315735‑315887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑