Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	311,367	T→G	L187V (TTG→GTG)	SAUSA300_RS01380 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	311,367	0	T	G	100.0%	48.5 / NA	20	L187V (TTG→GTG)	SAUSA300_RS01380	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/2);  total (18/2)

GCACCGTTCAATGGTTTAATATCTATGATTGTACCAACTAAGCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCTTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTGTAAAGCAAAATGATCATGTTGAAGCAGGGCAAACGTTGTTGCAATTCGACCAGC  >  NC_007793/311271‑311463                                                                                                 |                                                                                                 gCACTGTTCAATGGTTTAATATCTATGATTGTACCAACTAAGCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCGTgg                                                                                               <  1:3899543/100‑2 (MQ=255)     ctttCAATGGTTTAATATCTATGATTGTACCAACTAAGCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCGTGAATAc                                                                                           >  1:3872985/3‑100 (MQ=255)                                       caaGCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCttt                                                         >  1:4108178/2‑100 (MQ=255)                                       caaGCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCGTGAATACAGTTGACTTGGAAGGCAAAGGGTTCAAGTGCttt                                                         >  1:274553/2‑100 (MQ=255)                                        aaGCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTg                                                        >  1:114884/1‑100 (MQ=255)                                        aaGCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTg                                                        >  1:1527815/1‑100 (MQ=255)                                        aaGCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTg                                                        >  1:5292237/1‑100 (MQ=255)                                          gCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTGTa                                                      >  1:5595794/1‑100 (MQ=255)                                          ccATGCAGTTGGTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTGTa                                                      >  1:969236/2‑100 (MQ=255)                                                   tGGTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTGTAAAGCAAAAt                                             >  1:2429994/1‑100 (MQ=255)                                                    ggTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTGTAAAGCAAAATg                                            >  1:1401264/1‑100 (MQ=255)                                                              cAGAAGACGGTGTGGACATAGTCATTCATATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTGTAAAGCAAAATGATCATGTCGa                                  >  1:3986597/1‑100 (MQ=255)                                                                tatcaCGGTGTGGACATAGTCATTCATATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTGTAAAGCAAAATGATCATGTTGAAg                                >  1:1234783/5‑100 (MQ=255)                                                                acttaCGGTGTGGACATAGTCATTCATATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTGTAAAGCAAAATGATCACATTGAAg                                >  1:1190880/5‑100 (MQ=255)                                                                           ggACATAGTCATTCATATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTGTAAAGCAAAATGATCATGTTGAAGCAGGGCAAACg                     >  1:3878367/1‑100 (MQ=255)                                                                           ggACATAGTCATTCATATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTGTAAAGCAAAATGATCATGTTGAAGCAGGGCAAACg                     >  1:973755/1‑100 (MQ=255)                                                                                      cccatATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTGTAAAGCAAAATGATCATGTTGAAGCAGGGCAAACGTTGTTGCAAtt          >  1:2873389/3‑100 (MQ=255)                                                                                        catATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTGTAAAGCAAAATGATCATGTTGAAGCAGGGCAAACGTTGTTGCAATTCg        >  1:3303266/1‑100 (MQ=255)                                                                                             tGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTGTAAAGCAAAATGATCATGTTGAAGCAGGGCAAACGTTGTTGCAATTCGACCAg   >  1:5100220/1‑100 (MQ=255)                                                                                              ggCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTGTAAAGCAAAATGATCATGTTGAAGCAGGGCAAACGTTGTTGCAATTCGACCAGc  <  1:3559/100‑1 (MQ=255)                                                                                                 |                                                                                                 GCACCGTTCAATGGTTTAATATCTATGATTGTACCAACTAAGCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCTTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTTGTAAAGCAAAATGATCATGTTGAAGCAGGGCAAACGTTGTTGCAATTCGACCAGC  >  NC_007793/311271‑311463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑