Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	311,275	C→T	P156L (CCG→CTG)	SAUSA300_RS01380 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	311,275	0	C	T	100.0%	30.8 / NA	12	P156L (CCG→CTG)	SAUSA300_RS01380	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/4);  total (8/4)

TTTTTAGAGAGAAAATGGTAGGTGAAGGCTTAGCAATCAAAGCTCATGAAGAATCCAAAGTAATCGCACCGTTCAATGGTTTAATATCTATGATTGTACCAACTAAGCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCTTGA  >  NC_007793/311206‑311370                                                                      |                                                                                                tttttAGAGAGAAAATGGTAGGTGAAGGCTTAGCAATCAAAGCTCATGAAGAATCCAAAGTAATCGCACTGTTCAATGGTTTAATATCTATGATTGTAcc                                                                   <  1:2901491/100‑1 (MQ=255)         gagaAAATGGTAGGTGAAGGCTTAGCAATCAAAGCTCATGAAGAATCCAAAGTAATCGCACTGTTCAATGGTTTAATATCTATGATTGTACCAACTAAGc                                                           <  1:3969483/100‑1 (MQ=255)            aaaaTGGTAGGTGAAGGCTTAGCAATCAAAGCTCATGAAGAATCCAAAGTAATCGCACTGTTCAATGGTTTAATATCTATGATTGTACCAACTAAGCATg                                                        <  1:5291283/100‑1 (MQ=255)              caTGGTAGGTGAAGGCTTAGCAATCAAAGCTCATGAAGAATCCAAAGTAATCGCACTGTTCAATGGTTTAATATCTATGATTGTACCAACTAAGCATGCa                                                      >  1:5591580/2‑100 (MQ=255)                 ggTAGGTGAAGGCTTAGCAATCAAAGCTCATGAAGAATCCAAAGTAATCGCACTGTTCAATGGTTTAATATCTATGATTGTACCAACTAAGCATGCAGtt                                                   >  1:4543831/1‑100 (MQ=255)                    aGGTGAAGGCTTAGCAATCAAAGCTCATGAAGAATCCAAAGTAATCGCACTGTTCAATGGTTTAATATCTATGATTGTACCAACTAAGCATGCAGTTGGt                                                >  1:2663714/1‑100 (MQ=255)                               tAGCAATCAAAGCTCATGAAGAATCCAAAGTAATCGCACTGTTCAATGGTTTAATATCTCTGATTGTACCAACTAAGCATGCAGTTGGTATTCAATCaga                                     >  1:3724996/1‑100 (MQ=255)                                         tgCTCATGAAGAATCCAAAGTAATCGCACTGTTCAATGGTTTAATATCTATGATTGTACCAACTAAGCATGCAGTTGGTATTCAATCAGAAGACGGtgtg                           >  1:4034016/2‑100 (MQ=255)                                         aGCTCATGAAGAATCCAAAGTAATCGCACTGTTCAATGGTTTAATATCTATGATTGTACCAACTAAGCATGCAGTTGGTATTCAATCAGAAGACGGtgtg                           >  1:3140777/1‑100 (MQ=255)                                          ccTCATGAAGAATCCAAAGTAATCGCACTGTTCAATGGTTTAATATCTATGATTGTACCAACTAAGCATGCAGTTGGTATTCAATCAGAAGACGGTGTgg                          >  1:3799379/2‑100 (MQ=255)                                             cATGAAGAATCCAAAGTAATCGCACTGTTCAATGGTTTAATATCTATGATTGTACCAACTAAGCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGACa                       >  1:1521881/1‑100 (MQ=255)                                                                  gCACTGTTCAATGGTTTAATATCTATGATTGTACCAACTAAGCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCGTgg  <  1:3899543/100‑2 (MQ=255)                                                                      |                                                                                                TTTTTAGAGAGAAAATGGTAGGTGAAGGCTTAGCAATCAAAGCTCATGAAGAATCCAAAGTAATCGCACCGTTCAATGGTTTAATATCTATGATTGTACCAACTAAGCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGACATAGTCATTCATATTGGCTTGA  >  NC_007793/311206‑311370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑