| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 305,598 | A→T | D140V (GAT→GTT) | SAUSA300_RS01350 → | iron‑sulfur cluster repair protein ScdA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 305,598 | 0 | A | T | 96.3% | 71.4 / ‑3.7 | 27 | coding (419/675 nt) | SAUSA300_RS01350 | iron‑sulfur cluster repair protein ScdA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (25/1); minor base . (1/0); total (26/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GGACCTAATCATCCATATTTAGTTGAGTTAAAAGAAACATACGATACATTTAAAAATGGCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGATTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAGATGATTTAGTTTCAGACCACATTGC > NC_007793/305504‑305685 | ggACCTAATCATCCATATTTAGTTGAGTTAAAAGAAACATACGATACATTTAAAAATGGCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTc > 1:824782/1‑100 (MQ=255)ggACCTAATCATCCATATTTAGTTGAGTTAAAAGAAACATACGATACATTTAAAAATGGCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTc > 1:462638/1‑100 (MQ=255)ggACCTAATCATCCATATTTAGTTGAGTTAAAAGAAACATACGATACATTTAAAAATGGCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTc > 1:412183/1‑100 (MQ=255) gACCTAATCATCCATATTTAGTTGAGTTAAAAGAAACATACGATACATTTAAAAATGGCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTcc > 1:1588830/1‑100 (MQ=255) tAGTTGAGTTAAAAGAAACATACGATACATTTAAAAATGGCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGa > 1:1423180/1‑100 (MQ=255) gAGTTAAAAGAAACATACGATACATTTAAAAATGGCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAg > 1:3879980/1‑100 (MQ=255) aaaaaTGGCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACtg > 1:1403806/1‑100 (MQ=255) aTGGCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGAt < 1:2814975/100‑1 (MQ=255) aggCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATa > 1:3569799/2‑100 (MQ=255) ggCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAg > 1:1133214/1‑100 (MQ=255) ggCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAg > 1:752479/1‑100 (MQ=255) ggCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAg > 1:4060220/1‑100 (MQ=255) ggCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAg > 1:3500733/1‑100 (MQ=255) ggCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAg > 1:2913518/1‑100 (MQ=255) ggCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAg > 1:2269202/1‑100 (MQ=255) ggCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAg > 1:1456513/1‑100 (MQ=255) ggCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAg > 1:1277957/1‑100 (MQ=255) ggCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGAAGATATTAATACTGTGATAg > 1:3721534/1‑100 (MQ=255) ggCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGCAGTAGACGATATTAATACCGTGATAg > 1:572827/1‑100 (MQ=255) tAGAGCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAGATGAttt > 1:3182491/1‑100 (MQ=255) agCATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACCGTGATAgatcggaagag > 1:1886366/1‑92 (MQ=255) cATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAGATGATTTAGttt > 1:2320187/1‑100 (MQ=255) cATATGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAGATGATTTAGttt > 1:1055204/1‑100 (MQ=255) atGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAGATGATTTAGTTTCAg > 1:325131/1‑100 (MQ=255) atGCAAAAAGAAGACGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAGATGATTTAGTTTCAg > 1:2887717/1‑100 (MQ=255) atGCAAAAAGAAGACGATGTCG‑TTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAGATGATTTAGTTTCAGa > 1:4473389/1‑100 (MQ=255) aagaCGATGTCGTTTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAGATGATTTAGTTTCAGACCACATTGc > 1:4702274/1‑100 (MQ=255) | GGACCTAATCATCCATATTTAGTTGAGTTAAAAGAAACATACGATACATTTAAAAATGGCATGTTAGAGCATATGCAAAAAGAAGACGATGTCGATTTTCCAAAACTCATTAAATATGAGCAAGGTGAGGTAGTAGACGATATTAATACTGTGATAGATGATTTAGTTTCAGACCACATTGC > NC_007793/305504‑305685 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |