Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	291,848	A→G	H44R (CAT→CGT)	SAUSA300_RS01295 →	sorbitol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	291,848	0	A	G	100.0%	34.0 / NA	14	H44R (CAT→CGT)	SAUSA300_RS01295	sorbitol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/1);  total (13/1)

TGATAAAGAAGTTGCAACACCGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCATACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATTTTCTGGTGAAATCGTTGA  >  NC_007793/291768‑291920                                                                                 |                                                                         tGATAAAGAAGTTGCAACACCGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATtat                                                       >  1:6139/1‑100 (MQ=255)  gATAAAGAAGTTGCAACACCGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGTACAGATATTCGTACTTATGAAGGTCATtata                                                      >  1:2357260/1‑100 (MQ=255)     ggcgaagTTGCAACACCGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAg                                                   >  1:64897/4‑100 (MQ=255)              gCAACACCGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTc                                          >  1:5068964/1‑100 (MQ=255)                aaCACCGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCa                                        >  1:1552655/1‑100 (MQ=255)                 ttacCGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAg                                       >  1:3933563/3‑100 (MQ=255)                 ccacCGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAg                                       >  1:2724031/2‑100 (MQ=255)                   acCGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTg                                     <  1:5235466/100‑1 (MQ=255)                         aGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTa                               >  1:2423950/1‑100 (MQ=255)                         aGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTa                               >  1:3251803/1‑100 (MQ=255)                            actgtttctaaagATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGt                            >  1:208935/9‑100 (MQ=255)                                                      aTGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATTTTCTGGTGAGATCggaag  >  1:2082658/1‑96 (MQ=255)                                                      aTGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATTTTCTGGTGAAATCGTTGa  >  1:1448945/1‑100 (MQ=255)                                                      aTGCAGGAATTTGTGGCACAGATATTCGTACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATTTTCAGGTGAGATCggaag  >  1:1305783/1‑96 (MQ=255)                                                                                 |                                                                         TGATAAAGAAGTTGCAACACCGCTAGATGATAAAGTAAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCATACTTATGAAGGTCATTATAAAGTTAATTTTCCAGTGACATTAGGTCATGAATTTTCTGGTGAAATCGTTGA  >  NC_007793/291768‑291920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑