Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	291,130	G→A	L230L (TTG→TTA)	gatC →	PTS galactitol transporter subunit IIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	291,130	0	G	A	100.0%	30.0 / NA	12	L230L (TTG→TTA)	gatC	PTS galactitol transporter subunit IIC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/9);  total (3/9)

GTCTGAATAAGTTAGATGCTGATCCTGAAACAATTCAAAAACGTTTTGGCGCATTTGGAGAGTCTATATTTGTCGGCTTGATTTTAGGTTTAGGTATTGGTGTGTTAGCAGGTTACAAACCTGGAGACATCATTAATTTAGGAATGTCAATGGCTGCAGTAATGGTATTAATGCCT  >  NC_007793/291051‑291226                                                                                |                                                                                                 gTCTGAATAAGTTAGATGCTGATCCTGAAACAATTCAAAAACGTTTTGGCGCATTTGGAGAGTCTATATTTGTCGGCTTAATTTTAGGTTTAGGTATTgg                                                                              <  1:2926626/100‑1 (MQ=255)  tCTGAGTAAGTTAGATGCTGATCCTGAAACAATTCAAAAACGTTTTGGCGCATTTGGAGAGTCTATATTTGTCGGCTTAATTTTAGGTTTAGGTGTTGgt                                                                             <  1:1602927/100‑1 (MQ=255)     gAATAAGTTAGATGCTGATCCTGAAACAATTCAAAAACGTTTTGGCGCATTTGGAGAGTCTATATTTGTCGGCTTAATTTTAGGTTTAGGTATTGgtgtg                                                                          >  1:102233/1‑100 (MQ=255)       aTAAGTTAGATGCTGATCCTGAAACAATTCAAAAACGTTTTGGCGCATTTGGAGAGTCTATATTTGTCGGCTTAATTTTAGGTTTAGGTATTGGTGTGtt                                                                        <  1:1286328/100‑1 (MQ=255)       aTAAGTTAGATGCTGATCCTGAAACAATTCAAAAACGTTTTGGCGCATTTGGAGAGTCTATATTTGTCGGCTTAATTTTAGGTTTAGGTATTGGTGTGtt                                                                        <  1:4102291/100‑1 (MQ=255)            ttAGATGCTGATCCTGAAACAATTCAAAAACGTTTTGGCGCATTTGGAGAGTCTATATTTGTCGGCTTAATTTTAGGTTTAGGTATTGGTGTGTTAGCAg                                                                   <  1:576350/100‑1 (MQ=255)                  gCTGATCCTGAAACAATTCAAAAACGTTTTGGCGCATTTGGAGAGTCTATATTTGTCGGCTTAATTTTAGGTTTAGGTATTGGTGTGTTAGCAGGTTACa                                                             >  1:3868269/1‑100 (MQ=255)                           gAAACAATTCAAAAACGTTTTGGCGCATTTGGAGAGTCTATATTTGTCGGCTTAATTTTAGGTTTAGGTATTGGTGTGTTAGCAGGTTACAAACCTGgag                                                    >  1:5757681/1‑100 (MQ=255)                                                  cgcATTTGGAGAGTCTATATTTGTCGGCTTAATTTTAGGTTTAGGTATTGGTGTGTTAGCAGGTTACAAACCTGGAGACATCATTAATTTAGGAATGTCa                             <  1:2659314/100‑1 (MQ=255)                                                          gagaGTCTATATTTGTCGGCTTAATTTTAGGTTTAGGTATTGGTGTGTTAGCAGGTTACAAACCTGGAGACATCATTAATTTAGGAATGTCAATGGCTGc                     <  1:4294178/100‑1 (MQ=255)                                                           agagTCTATATTTGTCGGCTTAATTTTAGGTTTAGGTATTGGTGTGTTAGCAGGTTACAAACCTGGAGACATCATTAATTTAGGAATGTCAATGGCTGCa                    <  1:728465/100‑1 (MQ=255)                                                                             cTTAATTTTAGGTTTAGGTATTGGTGTGTTAGCAGGTTACAAACCTGGAGACATCATTAATTTAGGAATGTCAATGGCTGCAGTAATGGTATTAATGCCt  <  1:1528199/100‑1 (MQ=255)                                                                                |                                                                                                 GTCTGAATAAGTTAGATGCTGATCCTGAAACAATTCAAAAACGTTTTGGCGCATTTGGAGAGTCTATATTTGTCGGCTTGATTTTAGGTTTAGGTATTGGTGTGTTAGCAGGTTACAAACCTGGAGACATCATTAATTTAGGAATGTCAATGGCTGCAGTAATGGTATTAATGCCT  >  NC_007793/291051‑291226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑