Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	269,528	A→G	R164R (CGT→CGC)	SAUSA300_RS01195 ←	acetyl‑CoA acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	269,528	0	A	G	100.0%	13.5 / NA	7	R164R (CGT→CGC)	SAUSA300_RS01195	acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/5);  total (2/5)

GACCATCACGTTGTGCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCACGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCATATTGTAAGGTTGGATTGTT  >  NC_007793/269464‑269620                                                                 |                                                                                             gACCATCACGTTGTGCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTcagc                                                           <  1:2582797/100‑1 (MQ=255)     aTCACGTTGTGCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGtt                                                       >  1:1507899/1‑100 (MQ=255)             gtgCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCag                                               <  1:3089831/100‑2 (MQ=255)                                  gaCTTCTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATatc                          <  1:5210270/100‑1 (MQ=255)                                       cTGACAGCATAAGCATCTTGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCCCCTATATcatcat                     <  1:2001941/100‑1 (MQ=255)                                                         tGATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCATATTGTAAGGtagatcgga   >  1:542645/1‑92 (MQ=255)                                                          gATCTTCGCGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCATATTGTAAGGTTGGAttgtt  <  1:2369296/100‑1 (MQ=255)                                                                 |                                                                                             GACCATCACGTTGTGCGTCATAGGCACGTTGATGACTTCTGACAGCATAAGCATCTTGATCTTCACGTGATACGTCAAATTGGGATGCTACATTTTCAGCAGTTAAACCCATAGGATATGACGCACCTATATCATCATATTGTAAGGTTGGATTGTT  >  NC_007793/269464‑269620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑