Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	256,283	G→A	A435T (GCA→ACA)	SAUSA300_RS01135 →	antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	256,283	0	G	A	100.0%	38.2 / NA	15	A435T (GCA→ACA)	SAUSA300_RS01135	antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/11);  total (4/11)

GTTTGGCGGCTATTGCTGATCCAACACGTAACGGTTTAAACATCTTTGGATATACATTAAGTGGATGGACAGATGTTTTCATCGTCTTCTATGTTGCATTATTCCTAGGCATGATTCTATTAGGAATCGTTGCTTTCTATGAAGAAAAGAAAATTAGAAGTTTAAAAATTTAATATAAATCGGATTA  >  NC_007793/256188‑256374                                                                                                |                                                                                            gTTTGGCGGCTATTGCTGATCCAACACGTAACGGTTTAAACATCTTTGGATATACATTAAGTGGATGGACAGATGTTTTCATCGTCTTCTATGTTACatt                                                                                         <  1:1510675/100‑1 (MQ=255)           tATTGCTGATCCAACACGTAACGGTTTAAACATCTTTGGATATACATTAAGTGGATGGACAGATGTTTTCATCGTCTTCTATGTTACATTATTCCTAGGc                                                                               <  1:2012121/100‑1 (MQ=255)           tATTGCTGATCCAACACGTAACGGTTTAAACATCTTTGGATATACATTAAGTGGATGGACAGATGTTTTCATCGTCTTCTATGTTACATTATTCCTAGGc                                                                               <  1:5585051/100‑1 (MQ=255)           tATTGCTGATCCAACACGTAACGGTTTAAACATCTTTGGATATACATTAAGTGGATGGACAGATGTTTTCATCGTCTTCTATGTTACATTATTCCTAGGc                                                                               <  1:5761217/100‑1 (MQ=255)           tATTGCTGATCCAACACGTAACGGTTTAAACATCTTTGGATATACATTAAGTGGATGGACAGATGTTTTCATCGTCTTCTATGTTACATTATTCCTAGGc                                                                               <  1:900508/100‑1 (MQ=255)            tcttccgATCTATCACGTAACGGTTTAAACATCTTTGGATATACATTAAGTGGATGGACAGATGTTTTCATCGTCTTCTATGTTACATTATTCCTAGGCa                                                                              <  1:3601702/94‑1 (MQ=255)             ttGCTGATCCAACACGTAACGGTTTAAACATCCTTGGATATACATTAAGTGGATGGACAGATGTTTTCATCGTCTTCTATGTTACATTATTCCTAGGCag                                                                             <  1:5766050/100‑2 (MQ=255)              tGCTGATCCAACACGTAACGGTTTAAACATCTTTGGATATACATTAAGTGGATGGACAGATGTTTTCATCGTCTTCTATGTTACATTATTCCTAGGCATg                                                                            <  1:156999/100‑1 (MQ=255)              ctcTGATCCAACACGTAACGGTTTAAACATCTTTGGATATACATTAAGTGGATGGACAGATGTTTTCATCGTCTTCTATGTTACATTATTCCTAGGCATg                                                                            >  1:2872493/3‑100 (MQ=255)                 cgatctgacgcGTAACGGTTTAAACATCTTTGGATATACATTAAGTGGATGGACAGATGTTTTCATCGTCTTCTATGTTACATTATTCCTAGGCATGAtt                                                                         <  1:64775/90‑1 (MQ=255)                    tCCAACACGTAACGGTTTAAACATCTTTGGATATACATTAAGTGGATGGACAGATGTTTTCATCGTCTTCTATGTTACATTATTCCTAGGCATGATTCTa                                                                      >  1:3973498/1‑100 (MQ=255)                       aaCACGTAACGGTTTAAACATCTTTGGATATACATTAAGTGGATGGACAGATGTTTTCATCGTCTTCTATGTTACATTATTCCTAGGCATGATTCTATTa                                                                   >  1:2091391/1‑100 (MQ=255)                                                          tAAGTGGATGGACAGATGTTTTCATCGTCTTCTATGTTACATTATTCCTAGGCATGATTCTATTAGGAATCGTTGCTTTCTATGAAGAAAAGAAAATTAg                                >  1:1643118/1‑100 (MQ=255)                                                                             tttCATCGTCTTCTATGTTACATTATTCCTAGGCATGATTCTATTCGGAATCGTTGCTTTCTATGAAGAAAAGAAAATTAGAAGTTTAAAAATTTAatat             <  1:1866647/100‑1 (MQ=255)                                                                                        tctATGTTACATTATTCCTAGGCATGATTCTATTAGGAATCGTTGCTTTCTATGAAGAAAAGAAAATTAGAAGTTTAAAAATTTAATATAAATCGGATTa  <  1:2083455/100‑1 (MQ=255)                                                                                                |                                                                                            GTTTGGCGGCTATTGCTGATCCAACACGTAACGGTTTAAACATCTTTGGATATACATTAAGTGGATGGACAGATGTTTTCATCGTCTTCTATGTTGCATTATTCCTAGGCATGATTCTATTAGGAATCGTTGCTTTCTATGAAGAAAAGAAAATTAGAAGTTTAAAAATTTAATATAAATCGGATTA  >  NC_007793/256188‑256374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑