Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	240,358	T→A	N358K (AAT→AAA)	SAUSA300_RS01070 →	peptide ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	240,358	0	T	A	100.0%	58.8 / NA	22	N358K (AAT→AAA)	SAUSA300_RS01070	peptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (17/5);  total (17/5)

TGAGGACATCGATCAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAATTCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGACAGATCAATC  >  NC_007793/240274‑240435                                                                                     |                                                                              tGAGGACATCGATCAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGAt                                                                >  1:2948127/1‑100 (MQ=255)   aGGACATCGATCAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATgg                                                              >  1:3389691/1‑100 (MQ=255)        tccGATCTGATGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCaaa                                                         <  1:2772601/98‑1 (MQ=255)           gATCAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAg                                                      >  1:2663476/1‑100 (MQ=255)              cAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTaa                                                   >  1:1687822/1‑100 (MQ=255)                gTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAaat                                                 >  1:2506156/1‑100 (MQ=255)                       aaaGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAg                                          >  1:3326932/1‑100 (MQ=255)                            gaAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGt                                     <  1:942332/100‑1 (MQ=255)                                gccAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTAttt                                 >  1:3037157/3‑100 (MQ=255)                                gACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTAttt                                 >  1:4364485/1‑100 (MQ=255)                                 aCAAGGACGACAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTa                                >  1:625563/1‑100 (MQ=255)                                                      aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTAcccc           >  1:8386/1‑97 (MQ=255)                                                      aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACgcc           >  1:3079695/1‑98 (MQ=255)                                                      aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACgcc           >  1:3125608/1‑98 (MQ=255)                                                      aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc           >  1:3170592/1‑100 (MQ=255)                                                      aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc           >  1:5196310/1‑100 (MQ=255)                                                      aTGATAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGCCAAAAAGTAAATAATAAAGACGGCATTTATCAAATTGTTAAAATTACGAc           >  1:5235234/1‑100 (MQ=255)                                                      aTGATAAGATGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGAc           >  1:3800464/1‑100 (MQ=255)                                                          tAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGACAGAt       <  1:1102060/100‑1 (MQ=255)                                                          tAAGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGACAGAt       <  1:2505819/100‑1 (MQ=255)                                                           aaGTTGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGACAGATc      >  1:454762/1‑100 (MQ=255)                                                               tGATTAAAGCTTATAACGTCAAATCGTTAACGACAGATGGTCAAAAGGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGACAGATCAATc  <  1:4078704/100‑1 (MQ=255)                                                                                     |                                                                              TGAGGACATCGATCAGTTGCGTAAAGAGAAAGACAAGGACGATAAATCAATCTATGATAAGTTGATTAAAGCTTATAACGTCAATTCGTTAACGACAGATGGTCAAAAAGTAAATAATAAAGACGGTATTTATCAAATTGTTAAAATTACGACAGATCAATC  >  NC_007793/240274‑240435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑