Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	228,674	T→C	T21A (ACT→GCT)	SAUSA300_RS01030 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	228,674	0	T	C	100.0%	34.7 / NA	14	T21A (ACT→GCT)	SAUSA300_RS01030	membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/7);  total (7/7)

GTAGGTAGTAGTACATAAAGAGTATGGCTGCGAACATGCCGAATATTGAAGTGAAGTTACCTACAGTGACTGCCATGTAATGATTTAAAGTTAAATAGTAACTTAGACTAATAATAAAAAATAAAATGATTTGATAGGTAATTAACACCATTCAAGTCCCTCCATTAATCGTAGACA  >  NC_007793/228584‑228760                                                                                           |                                                                                       ataggtagTAGTACATAAAGAGTATGGCTGCGAACATGCCGAATATTGAAGTGAAGTTACCTACAGTGACTGCCATGTAATGATTTAAAGCTAAATAGTa                                                                               >  1:4881903/2‑100 (MQ=255)                        aTGGCTGCGAACATGCCGAATATTGAAGTGAAGTTACCTACAGTGACTGCCATGTAATGATTTAAAGCTAAATAGTAACTTAGACTAATaataaaaaata                                                        >  1:389972/1‑100 (MQ=255)                                 aaCATGCCGAATATTGAAGTGAAGTTACCTACAGTGACTGCCATGTAATGATTTAAAGCTAAATAGTAACTTAGACTAATAATAAAAAATAAAATGAttg                                               <  1:17755/100‑2 (MQ=255)                                       ccGAATATTGAAGTGAAGTTACCTACAGTGACTGCCATGTAATGATTTAAAGCTAAATAGTAACTTAGACTAATAATAAAAAATAAAATGATTTGATAgg                                         >  1:4636328/1‑100 (MQ=255)                                                          tACCTACAGTGACTGCCATGTAATGATTTAAAGCTAAATAGTAACTTAGACTAATAATAAAAAATAAAATGATTTGATAGGTAATTAACACCATTCAAGt                      >  1:2418596/1‑100 (MQ=255)                                                               aCAGTGACTGCCATGTAATGATTTAAAGCTAAATAGTAACTTAGACTAATAATAAAAAATAAAATGATTTGATAGGTAATTAACACCATTCAAGTCCCTc                 >  1:3026762/1‑100 (MQ=255)                                                                  gTGACTGCCATGTAATGATTTAAAGCTAAATAGGAACTTAGACTAATAATAAAAAATAAAATGATTTGATAGGTAATTAACACCATTCAAGTCCCTCCAt              >  1:3952807/1‑100 (MQ=255)                                                                        gCCATGTAATGATTTAAAGCTAAATAGTAACTTAGACTAATAATAAAAAATAAAATGATTTGATAGGTAATTAACACCATTCAAGTCCCTCCATTAATCg        >  1:1360547/1‑100 (MQ=255)                                                                         ccATGTAATGATTTAAAGCTAAATAGTAACTTAGACTAATAATAAAAAATAAAATGATTTGATAGGTAATTAACACCATTCAAGTCCCTCCATTAATCGt       <  1:1642392/100‑1 (MQ=255)                                                                         ccATGTAATGATTTAAAGCTAAATAGTAACTTAGACTAATAATAAAAAATAAAATGATTTGATAGGTAATTAACACCATTCAAGTCCCTCCATTAATCGt       <  1:2381050/100‑1 (MQ=255)                                                                         ccATGTAATGATTTAAAGCTAAATAGTAACTTAGACTAATAATAAAAAATAAAATGATTTGATAGGTAATTAACACCATTCAAGTCCCTCCATTAATCGt       <  1:3246583/100‑1 (MQ=255)                                                                         ccATGTAATGATTTAAAGCTAAATAGTAACTTAGACTAATAATAAAAAATAAAATGATTTGATAGGTAATTAACACCATTCAAGTCCCTCCATTAATCGt       <  1:682495/100‑1 (MQ=255)                                                                           aTGTAATGATTTAAAGCTAAATAGTAACTTAGACTAATAATAAAAAATAAAATGATTTGATAGGTAATTAACACCATTCAAGTCCCTCCATTAATCGTAg     <  1:4886893/100‑1 (MQ=255)                                                                              tAATGATTTAAAGCTAAATAGTAACTTAGACTAATAATAAAAAATAAAATGATTTGATAGGTAATTAACACCATTCAAGTCCCTCCATTAATCGTAGACa  <  1:1426522/100‑1 (MQ=255)                                                                                           |                                                                                       GTAGGTAGTAGTACATAAAGAGTATGGCTGCGAACATGCCGAATATTGAAGTGAAGTTACCTACAGTGACTGCCATGTAATGATTTAAAGTTAAATAGTAACTTAGACTAATAATAAAAAATAAAATGATTTGATAGGTAATTAACACCATTCAAGTCCCTCCATTAATCGTAGACA  >  NC_007793/228584‑228760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑