Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	225,548	C→T	P177L (CCA→CTA)	murQ →	N‑acetylmuramic acid 6‑phosphate etherase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	225,548	0	C	T	100.0%	20.0 / NA	9	P177L (CCA→CTA)	murQ	N‑acetylmuramic acid 6‑phosphate etherase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/0);  total (9/0)

AACACAATCGGTGCTACAACAGTATCTATTTCATGCAATGAACATGCAGTTATAAGTGAAATTGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAA  >  NC_007793/225475‑225640                                                                          |                                                                                             aaCACAATCGGTGCTACAACAGTATCTATTTCATGCAATGAACATGCAGTTATAAGTGAAATTGCGCAGTATCTAGTAGAAGTTAAAGTTGGTCCAGAAg                                                                    >  1:161826/1‑100 (MQ=255) aaCACAATCGGTGCTACAACAGTATCTATTTCATGCAATGAACATGCAGTTATAAGTGAAATTGCGCAGTATCTAGTAGAAGTTAAAGTTGGTCCAGAAg                                                                    >  1:2574497/1‑100 (MQ=255) aaCACAATCGGTGCTACAACAGTATCTATTTCATGCAATGAACATGCAGTTATAAGTGAAATTGCGCAGTATCTAGTAGAAGTTAAAGTTGGTCCAGAAg                                                                    >  1:3533736/1‑100 (MQ=255)                acaacaGTATCTATTTCATGCAATGAACATGCAGTTATAAGTGAAATTGCGCAGTATCTAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCaa                                                     >  1:5279809/1‑100 (MQ=255)                  gacaGTATCTATTTCATGCAATGAACATGCAGTTATAAGTGAAATTGCGCAGTATCTAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAaca                                                   >  1:85434/2‑100 (MQ=255)                  aacaGTATCTATTTCATGCAATGAACATGCAGTTATAAGTGAAATTGCGCAGTATCTAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAaca                                                   >  1:1486919/1‑100 (MQ=255)                                              gCAGTTATAAGTGAAATTGCGCAGTATCTAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGCTCAACACGTTTAAAGTCTGGTACAGCACAAAAgc                       >  1:4594212/1‑99 (MQ=255)                                                                acgcAGTATCTAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGAttt     >  1:5066590/2‑100 (MQ=255)                                                                   cAGTATCTAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCaa  >  1:2499649/1‑100 (MQ=255)                                                                          |                                                                                             AACACAATCGGTGCTACAACAGTATCTATTTCATGCAATGAACATGCAGTTATAAGTGAAATTGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAA  >  NC_007793/225475‑225640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑