Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	214,514	T→C	G44G (GGA→GGG)	SAUSA300_RS00970 ←	arginine biosynthesis bifunctional protein ArgJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	214,514	0	T	C	100.0%	35.4 / NA	15	G44G (GGA→GGG)	SAUSA300_RS00970	arginine biosynthesis bifunctional protein ArgJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/13);  total (2/13)

TTGTAAAGTGTCTTCAGTGACAATAAGTGGTGCAGCTTTAAACTGATTTAAAGTATATACGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCATCCAAAGTCTTTTTTGTTAGCGCGTAAACCGATGTGCATACCACCAGCCGTGAAGCCTTGAGGTGTACTG  >  NC_007793/214418‑214583                                                                                                 |                                                                      ttGTAAAGTGTCTTCAGTGACAATAAGTGGTGCAGCTTTAAACTGATTTAAAGTATATACGGCAGCTGCACTTGCCAAAGACGATGAGTAAAtccaccca                                                                    <  1:1810229/100‑1 (MQ=255)  tGTAAAGTGTCTTCAGTGACAATAAGTGGTGCAGCTTTAAACTGATTTAAAGTATATACGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCACCCaa                                                                   <  1:2147860/100‑1 (MQ=255)   gTAAAGTGTCTTCAGTGACAATAAGTGGTGCGGCTTTAAACTGATTTAAAGTATATACGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCACCCaat                                                                  <  1:3335050/100‑2 (MQ=255)               cAGTGACAATAAGTGGTGCAGCTTTAAACTGATTTAAAGTATATACGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCACCCAAAGTCTTTTTTGtt                                                      <  1:4525563/100‑1 (MQ=255)                 gTGACAATAAGTGGTGCAGCTTTAAACTGATTTAAAGTATATACGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCACCCAAAGTCTTTTTTGTTAg                                                    >  1:1270693/1‑100 (MQ=255)                    aCAATAAGTGGTGCAGCTTTAAACTGATTTAAAGTATATACGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCACCCAAAGTCTTTTTTGTCAgcgc                                                 >  1:5401737/1‑100 (MQ=255)                     cAATAAGTGGTGCAGCTTTAAACTGATTTAAAGTATATGCGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCACCCAAAGTCTTTTTTG‑TAGCGCGt                                               <  1:581661/100‑1 (MQ=255)                                         aaCTGATTTAAAGTATATACGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCACCCAAAGTC‑TTTTTGTTAGCGCGTAAACCGATGTGCATaccacc                           <  1:3588279/100‑1 (MQ=255)                                            tGATTTAAAGTATATACGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCACCCAAAGTC‑TTTTTGTTAGCGCGTAAACCGATGTGCATACCACCAgg                        <  1:3128073/100‑2 (MQ=255)                                                ttAAAGTATATACGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCACCCAAAGTCTTTTTTGTTAGCGCGTAAACCGATGTGCATACCACCAGCCGt                     <  1:4460443/100‑1 (MQ=255)                                                 tAAAGTATATACGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCACCCAAAGTCTTTTTTGTTAGCGCGTAAACCGATGTGCATACCACCAGCCGtt                    <  1:4517607/100‑2 (MQ=255)                                                 tAAAGTATATACGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCACCCAAAGTCTTTTTTGTTAGCGCGTAAACCGATGTGCATACCACCAGCCGTg                    <  1:4138767/100‑1 (MQ=255)                                                  aaaGTATATACGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCACCCAAAGTCTTTTTTGTTAGCGCGTAAACCGATGTGCATACCACCAGCCGTGa                   <  1:3482137/100‑1 (MQ=255)                                                    aGTATATACGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCACCCAAAGTCTTTTTTGTTAGCGCGTAAACCGATGTGCATACCACCAGCCGTGagg                 <  1:1744994/100‑3 (MQ=255)                                                         ataCGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCACCCAAAGTCTTTTTTGTTAGCGCGTAAACCGATGTGCATACCACCAGCCGTGAAGCCTTg            <  1:719818/100‑1 (MQ=255)                                                                   tGCACTTGCCAAAGACGATGAGTAAATCCACCCAAAGTCTTTTTTGTTAGCGCGTAAACCGATGTGCATACCACCAGCCGTGAAGCCTTGAGGTGTACTg  <  1:4693270/100‑1 (MQ=255)                                                                                                 |                                                                      TTGTAAAGTGTCTTCAGTGACAATAAGTGGTGCAGCTTTAAACTGATTTAAAGTATATACGGCAGCTGCACTTGCCAAAGACGATGAGTAAATCCATCCAAAGTCTTTTTTGTTAGCGCGTAAACCGATGTGCATACCACCAGCCGTGAAGCCTTGAGGTGTACTG  >  NC_007793/214418‑214583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑