Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	205,448	A→C	R578R (CGA→CGC)	SAUSA300_RS00950 →	non‑ribosomal peptide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	205,448	0	A	C	100.0%	61.7 / NA	25	R578R (CGA→CGC)	SAUSA300_RS00950	non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/3);  total (22/3)

GTAACACGTTAGAAGATCATGCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGATTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAGACGACGATTTTGTTATCAGGAACTATAGCCTTTGATGCTGCAAC  >  NC_007793/205354‑205531                                                                                               |                                                                                    gTAACACGTTAGAAGATCATGCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGt                                                                                >  1:1420551/1‑100 (MQ=255) gTAACACGTTAGAAGATCATGCTTATGTTATTTACACGTCGGGGACAACTGGCAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGt                                                                                >  1:4135992/1‑100 (MQ=255)          tAGAAGATCATGCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCaaaa                                                                       >  1:483345/1‑100 (MQ=255)                   ttGCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGt                                                              >  1:5323677/2‑100 (MQ=255)                     gCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTAc                                                            >  1:3106313/1‑100 (MQ=255)                     gCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTAc                                                            >  1:3430820/1‑100 (MQ=255)                     gCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTAc                                                            >  1:4576329/1‑100 (MQ=255)                      cTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTacgacag                                                           >  1:1070243/1‑94 (MQ=255)                        tATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCAt                                                         >  1:3942837/1‑100 (MQ=255)                           gctATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTaa                                                      >  1:5025944/3‑100 (MQ=255)                                 tACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAag                                                <  1:5371141/100‑1 (MQ=255)                                 tACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAag                                                >  1:3052190/1‑100 (MQ=255)                                  acacGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAaga                                               >  1:2151928/1‑100 (MQ=255)                                  acacGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAaga                                               >  1:4503832/1‑100 (MQ=255)                                         ggggACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAgagatcgga                                        >  1:1270548/1‑95 (MQ=255)                                          gggACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAgagatcggaa                                       >  1:4239213/1‑94 (MQ=255)                                          gggACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAGAcgacga                                       >  1:825431/1‑100 (MQ=255)                                          gggACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAAAGAAgagatcggaa                                       >  1:480293/1‑94 (MQ=255)                                             aCAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAGACGACGAttt                                    >  1:5319491/1‑100 (MQ=255)                                              cAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAgacagatcggaa                                   >  1:5155755/1‑91 (MQ=255)                                                     caaCCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAGACGACGATTTTGTTATCa                            >  1:1754070/2‑100 (MQ=255)                                                               gcgACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAGACGACGATTTTGTTATCAGGAACTATAg                  >  1:5572518/3‑100 (MQ=255)                                                                 gACACTAATTCCGCACCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAGACGACGATTTTGTTATCAGGAACTATAGcc                >  1:4601642/1‑100 (MQ=255)                                                                              ctccGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAGACGACGATTTTGTTATCAGGAACTATAGCCTTTGATGCTGCaa   <  1:2885624/98‑1 (MQ=255)                                                                               aCCGAGGTATTGTTCGCTTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAGACGACGATTTTGTTATCAGGAACTATAGCCTTTGATGCTGCAAc  <  1:727539/100‑1 (MQ=255)                                                                                               |                                                                                    GTAACACGTTAGAAGATCATGCTTATGTTATTTACACGTCGGGGACAACTGGTAACCCTAAAGGGACACTAATTCCGCACCGAGGTATTGTTCGATTGGTCCATCAAAATCATTATGTACCATTAAATGAAGAGACGACGATTTTGTTATCAGGAACTATAGCCTTTGATGCTGCAAC  >  NC_007793/205354‑205531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑