Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	200,717	G→A	E37E (GAG→GAA)	SAUSA300_RS00940 →	formate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	200,717	0	G	A	100.0%	19.7 / NA	9	E37E (GAG→GAA)	SAUSA300_RS00940	formate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/0);  total (9/0)

AGCATTATTTCCAGAAGCAGTAGAAGGTCAAGAAAATCAATTACTTAATACTAAAAAAGCATTAGGATTAAAAACATTTTTAGAGGAAAGAGGACATGAGTTCATTATATTAGCAGATAATGGTGAAGACTTAGATAAACATTTACCAGATATGGATGTGATTATTAGTGCGCCATTTTATCCTGCATATATGA  >  NC_007793/200618‑200811                                                                                                    |                                                                                               tgCATTATTTCCAGAAGCAGTAGAATGTCAAGAAAATCAATTACTTAATACTAAAAAAGCATTAGGATTAAAAACATTTTTAGAGGAAAGAGGACATGAg                                                                                                >  1:4613870/2‑100 (MQ=255)          cccAGAAGCAGTAGAAGGTCAAGAAAATCAATTACTTAATACTAAAAAAGCATTAGGATTAAAAACATTTTTAGAGGAAAGAGGACATGAATTCATtata                                                                                       >  1:5456505/2‑100 (MQ=255)           ccAGAAGCAGTAGAAGGTCAAGAAAATCAATTACTTAATACTAAAAAAGCATTAGGATTAAAAACATTTTTAGAGGAAAGAGGACATGAATTCATTatat                                                                                      >  1:988168/1‑100 (MQ=255)           acAGAAGCAGTAGAAGGGCAAGAAAATCAATTACTTAATACTAAAAAAGCATTAGGATTAAAAACATTTTTAGAGGAAAGAGGACATGAATTCATTatat                                                                                      >  1:5193703/2‑100 (MQ=255)            cAGAAGCAGTAGAAGGTCAAGAAAATCAATTACTTAATACTAAAAAAGCATTAGGATTAAAAACATTTTTAGAGGAAAGAGGACATGAATTCATTATAtt                                                                                     >  1:2622692/1‑100 (MQ=255)                                                       aaaaGCATTAGGATTAAAAACATTTTTAGAGGAAAGAGGACATGAATTCATTATATTAGCAGATAATGGTGAAGACTTAGATAAACATTTACCAGATATg                                          >  1:2183698/1‑100 (MQ=255)                                                        caaGCATTAGGATTAAAAACATTTTTAGAGGAAAGAGGACATGAATTCATTATATTAGCAGATAATGGTGAAGACTTAGATAAACATTTACCAGATATgg                                         >  1:5026590/2‑100 (MQ=255)                                                                        aaaCATTTTTAGAGGAAAGAGGACATGAATTCATTATATTAGCAGATAATGGTGAAGACTTAGATAAACATTTACCAGATATGGATGTGATTATTAGTgc                         >  1:815189/1‑100 (MQ=255)                                                                                           agGACATGAATTCATTATATTAGCAGATAATGGTGAAGACTTAGATAAACATTTACCAGATATGGATGTGATTATTAGTGCGCCATTTTATCCTGCatat      >  1:5206883/1‑100 (MQ=255)                                                                                               tgtGAATTCATTATATTAGCAGATAATGGTGAAGACTTAGATAAACATTTACCAGATATGGATGTGATTATTAGTGCGCCATTTTATCCTGCATATATGa  >  1:2406416/3‑100 (MQ=255)                                                                                                    |                                                                                               AGCATTATTTCCAGAAGCAGTAGAAGGTCAAGAAAATCAATTACTTAATACTAAAAAAGCATTAGGATTAAAAACATTTTTAGAGGAAAGAGGACATGAGTTCATTATATTAGCAGATAATGGTGAAGACTTAGATAAACATTTACCAGATATGGATGTGATTATTAGTGCGCCATTTTATCCTGCATATATGA  >  NC_007793/200618‑200811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑