Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	180,763	C→T	L160F (CTC→TTC)	SAUSA300_RS00835 →	UDP‑N‑acetyl glucosamine 2‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	180,763	0	C	T	100.0%	27.0 / NA	11	L160F (CTC→TTC)	SAUSA300_RS00835	UDP‑N‑acetyl glucosamine 2‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/3);  total (8/3)

TGTACCTGAAGAAATCAATCGTAAAATTGTTGACCATGTCAGTGATGTGAATCTACCTTATACGGAACATAGCAGACGTTATTTATTAGATGAAGGCCTCAATAAAGCGAATATCTTTGTGACAGGATCACCGATGACAGAAGTGATAGAAGCGCATCGAGATAAAATTAATCACAGTGACGTTTTAAATAA  >  NC_007793/180666‑180857                                                                                                  |                                                                                               tGTACCTGAAGAAATCAATCGTAAAATTGTTGACCATGTCAGTGATGTGAATCTACCTTATGCGGAACATAGCAGACGTTATTTATTAGTTGAAGGCTTc                                                                                              >  1:1177597/1‑100 (MQ=255)                   ccGTAAAATTGTTGACCATGTCAGTGATGTGAATCTACCTTATACGGAACATAGCAGACGTTATTTATTAGATGAAGGCTTCAATAAAGCGAATATCttt                                                                            >  1:5617149/2‑100 (MQ=255)                                                ccgaTCTGCCTTATACGGAACATAGCAGACGTTATTTATTAGATGAAGGCTTCAATAAAGCGAATATCTTTGTGACAGGATCACCGATGACAGAAGTGAt                                               <  1:2529715/97‑1 (MQ=255)                                                    gcTACCTTATACGGAACATAGCAGACGTTATTTATTAGATGAAGGCTTCAATAAAGCGAATATCTTTGTGACAGGATCACCGATGACAGAAGTGATAGaa                                           >  1:243314/2‑100 (MQ=255)                                                       aCCTTATACGGAACATAGCAGACGTTATTTATTAGATGAAGGCTTCAATAAAGCGAATATCTTTGTGACAGGATCACCGATGACAGAAGTGATAGAAgcg                                        >  1:3543501/1‑100 (MQ=255)                                                            ataCGGAACATAGCAGACGTTATTTATTAGATGAAGGCTTCAATAAAGCGAATATCTTTGTGACAGGATCACCGATGACAGAAGTGATAGAAGCGCATCg                                   <  1:4638121/100‑1 (MQ=255)                                                                  caCATAGCAGACGTTATTTATTAGATGAAGGCTTCAATAAAGCGAATATCTTTGTGACAGGATCACCGATGACAGAAGTGATAGAAGCGCATCGAGATaa                             >  1:813504/2‑100 (MQ=255)                                                                     aTAGCAGACGTTATTTATTAGATGAAGGCTTCAATAAAGCGAATATCTTTGTGACAGGATCACCGATGACAGAAGTGATAGAAGCGCATCGAGATAAAAt                          >  1:437596/1‑100 (MQ=255)                                                                      tAGCAGACGTTATTTATTAGATGAAGGCTTCAATAAAGCGAATATCTTTGTGACAGGATCACCGATGACAGAAGTGATAGAAGCGCATCGAGATAAAAtt                         >  1:2891483/1‑100 (MQ=255)                                                                            aCGTTATTTATTAGATGAAGGCTTCAATAAAGCGAATATCTTTGTGACAGGATCACCGATGACAGAAGTGATAGAAGCGCATCGAGATAAAATTAATcac                   <  1:76691/100‑1 (MQ=255)                                                                                             aaGGCTTCAATAAAGCGAATATCTTTGTGACAGGATCACCGATGACAGAAGTGATAGAAGCGCATCGAGATAAAATTAATCACAGTGACGTTTtaaataa  >  1:876091/1‑100 (MQ=255)                                                                                                  |                                                                                               TGTACCTGAAGAAATCAATCGTAAAATTGTTGACCATGTCAGTGATGTGAATCTACCTTATACGGAACATAGCAGACGTTATTTATTAGATGAAGGCCTCAATAAAGCGAATATCTTTGTGACAGGATCACCGATGACAGAAGTGATAGAAGCGCATCGAGATAAAATTAATCACAGTGACGTTTTAAATAA  >  NC_007793/180666‑180857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑