Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	178,354	T→G	Y75D (TAT→GAT)	SAUSA300_RS00825 →	UDP‑glucose 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	178,354	0	T	G	100.0%	22.6 / NA	10	Y75D (TAT→GAT)	SAUSA300_RS00825	UDP‑glucose 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (5/5);  total (5/5)

AAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTG  >  NC_007793/178271‑178422                                                                                    |                                                                     aaaaaTATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATgc                                                      <  1:712665/100‑1 (MQ=255)                        aaGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGt                               <  1:4808723/100‑1 (MQ=255)                              tACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACTAGTGCCGTCAtgtg                         <  1:5191624/100‑1 (MQ=255)                               aCATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGa                        >  1:2525318/1‑100 (MQ=255)                                cATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGaa                       >  1:1934148/1‑100 (MQ=255)                                          gtgCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAg             >  1:5244249/1‑100 (MQ=255)                                             cGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTg          <  1:4602111/100‑1 (MQ=255)                                               tGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAg        >  1:3890615/1‑100 (MQ=255)                                                gATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTACATGCAGCAGCTTTAAAAAAAGTGCCGTCATGTGAATTCTTTCAAGTTGAgg       >  1:4121865/1‑100 (MQ=255)                                                     tCAAAGTGTAGAAACAGCAATGCGAGATGTTGATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTg  <  1:4595108/100‑1 (MQ=255)                                                                                    |                                                                     AAAAATATAATAATTCAAAATTAAAGTTCTACATTGGTGATGTGCGTGATAGTCAAAGTGTAGAAACAGCAATGCGAGATGTTTATTACGTATTCCATGCAGCAGCTTTAAAACAAGTGCCGTCATGTGAATTCTTTCCAGTTGAGGCAGTG  >  NC_007793/178271‑178422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑