Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 141,817 G→A Q212Q (CAG→CAA)  SAUSA300_RS00635 → siderophore biosynthesis protein SbnF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007793141,8170GA99.6% 754.9 / NA 264Q212Q (CAG→CAA) SAUSA300_RS00635siderophore biosynthesis protein SbnF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (261/1);  minor base T (1/0);  total (263/1)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 275 total aligned reads displayed.

AGATAAAGTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAGATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTGAAGAA  >  NC_007793/141721‑141913
                                                                                                |                                                                                                
aGATAAAGTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAAtt                                                                                               >  1:4244668/1‑100 (MQ=255)
  gtAAAGTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGa                                                                                             >  1:3343706/2‑100 (MQ=255)
    aaaGTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAg                                                                                           >  1:1045265/1‑100 (MQ=255)
    aaaGTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAg                                                                                           >  1:5211588/1‑100 (MQ=255)
    aaaGTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAg                                                                                           >  1:1289621/1‑100 (MQ=255)
     aaGTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGc                                                                                          >  1:1713968/1‑100 (MQ=255)
         aGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCt                                                                                      >  1:3180429/1‑100 (MQ=255)
         aGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCt                                                                                      >  1:1472517/1‑100 (MQ=255)
         aGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCt                                                                                      >  1:2557836/1‑100 (MQ=255)
           taaCGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTgg                                                                                    >  1:5541481/2‑100 (MQ=255)
           aaaCGACGGTATCAAGAAGTGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTgg                                                                                    >  1:354145/1‑100 (MQ=255)
            tacgacgGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGc                                                                                   >  1:345775/2‑100 (MQ=255)
            aaCGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGc                                                                                   >  1:738003/1‑100 (MQ=255)
             cgacgccgaTCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCa                                                                                  >  1:5780074/9‑100 (MQ=255)
             ccgacgGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCa                                                                                  >  1:2314101/2‑100 (MQ=255)
             acgacgGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCa                                                                                  >  1:5477764/1‑100 (MQ=255)
              tgccgGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCaa                                                                                 >  1:32816/4‑100 (MQ=255)
              cgacgGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCaa                                                                                 >  1:293294/1‑100 (MQ=255)
                gcgGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAAc                                                                               >  1:1304755/2‑100 (MQ=255)
                       cAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTaaa                                                                        >  1:5129190/1‑100 (MQ=255)
                                gtgtAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTga                                                               >  1:3993794/2‑100 (MQ=255)
                                  gtAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTgaga                                                             >  1:4725064/1‑100 (MQ=255)
                                     actAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAgatga                                                          >  1:3043689/3‑100 (MQ=255)
                                        aaCGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATAc                                                       >  1:506294/1‑100 (MQ=255)
                                              acaccgcGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTac                                                 >  1:3036736/7‑100 (MQ=255)
                                                       gtgcaaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGc                                        >  1:3616682/4‑100 (MQ=255)
                                                       caacaaGTTGGCGATAAGATTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGc                                        >  1:1528687/1‑100 (MQ=255)
                                                       caacaaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATgcc                                        >  1:2882157/1‑98 (MQ=255)
                                                       caacaaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGc                                        >  1:2989493/1‑100 (MQ=255)
                                                       caacaaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGc                                        >  1:3373413/1‑100 (MQ=255)
                                                       caacaaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGc                                        >  1:1230765/1‑100 (MQ=255)
                                                       caacaaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGc                                        >  1:5011878/1‑100 (MQ=255)
                                                       caacaaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGc                                        >  1:2036617/1‑100 (MQ=255)
                                                       caacaaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGc                                        >  1:4822699/1‑100 (MQ=255)
                                                        aacaaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCa                                       >  1:3677542/1‑100 (MQ=255)
                                                        aacaaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCa                                       >  1:4405277/1‑100 (MQ=255)
                                                         acaaGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAg                                      >  1:3903604/1‑100 (MQ=255)
                                                           ctgTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAgat                                    >  1:1787334/3‑98 (MQ=255)
                                                           ctgTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGCCAgat                                    >  1:4543897/3‑98 (MQ=255)
                                                            tgTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGttt                                   >  1:2748613/2‑100 (MQ=255)
                                                            atttGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGttt                                   >  1:5702659/3‑100 (MQ=255)
                                                            aGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAgatc                                   >  1:4268907/1‑97 (MQ=255)
                                                            aGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGttt                                   >  1:4519875/1‑100 (MQ=255)
                                                            aGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGttt                                   >  1:625776/1‑100 (MQ=255)
                                                            aGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGttt                                   >  1:3251071/1‑100 (MQ=255)
                                                            aGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGttt                                   >  1:3275415/1‑100 (MQ=255)
                                                            aGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGttt                                   >  1:894461/1‑100 (MQ=255)
                                                            aGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATAGCAgatc                                   >  1:981256/1‑97 (MQ=255)
                                                             tttGGAGATAAGACTTATGAACATTTTGTAAAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTg                                  >  1:1444796/2‑100 (MQ=255)
                                                             gTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTg                                  >  1:4914826/1‑100 (MQ=255)
                                                             cttGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTg                                  >  1:2701786/2‑100 (MQ=255)
                                                             cttGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTg                                  >  1:497247/2‑100 (MQ=255)
                                                             cctGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTg                                  >  1:606096/3‑100 (MQ=255)
                                                             attGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTg                                  >  1:4135778/2‑100 (MQ=255)
                                                              ttGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGa                                 >  1:4459279/1‑100 (MQ=255)
                                                              ttGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGa                                 >  1:5575130/1‑100 (MQ=255)
                                                              ttGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGa                                 >  1:1771901/1‑100 (MQ=255)
                                                              ttGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGa                                 >  1:1937327/1‑100 (MQ=255)
                                                              ttGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATAGCAGTTTGa                                 >  1:1866380/1‑100 (MQ=255)
                                                              ttGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGCACACCCATGGCAGTTTGa                                 >  1:2049948/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGCAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:2903747/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:5044086/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:1794193/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:1690558/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:562833/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:1140870/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:2120924/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:3481858/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:3780403/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:262615/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:3924263/1‑100 (MQ=255)
                                                               tGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGaa                                >  1:3583363/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAAc                               >  1:2829881/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAAc                               >  1:3757777/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAAc                               >  1:5399018/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAAc                               >  1:5265579/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAAc                               >  1:2010430/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAAc                               >  1:5740794/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAAc                               >  1:1398426/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAAc                               >  1:4758298/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAAc                               >  1:809895/1‑100 (MQ=255)
                                                                ggCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAAc                               >  1:4584368/1‑100 (MQ=255)
                                                                     tAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTc                          >  1:2064069/1‑100 (MQ=255)
                                                                      aaGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCa                         >  1:1469989/1‑100 (MQ=255)
                                                                      aaGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCa                         >  1:5636408/1‑100 (MQ=255)
                                                                      aaGACTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCa                         >  1:2675490/1‑100 (MQ=255)
                                                                       ccccTTATGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCAt                        >  1:4070904/4‑100 (MQ=255)
                                                                             ctGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGt                  >  1:1093287/2‑100 (MQ=255)
                                                                             aTGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGt                  >  1:2499335/1‑100 (MQ=255)
                                                                              tGAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGtt                 >  1:823425/1‑100 (MQ=255)
                                                                               gAACATTTTGTACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTg                >  1:4860715/1‑100 (MQ=255)
                                                                                        atACAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTg       >  1:4692039/2‑100 (MQ=255)
                                                                                         caCAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGGGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTga      >  1:3637847/2‑100 (MQ=255)
                                                                                          aCAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTgaa     >  1:4339/1‑100 (MQ=255)
                                                                                          aCAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTgaa     >  1:2257124/1‑100 (MQ=255)
                                                                                          aCAGCAAATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTgaa     >  1:2217963/1‑100 (MQ=255)
                                                                                           tagcaaATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTgaag    >  1:240336/2‑100 (MQ=255)
                                                                                           cagcaaATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTgaag    >  1:3723177/1‑100 (MQ=255)
                                                                                            agcaaATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTgaaga   >  1:249393/1‑100 (MQ=255)
                                                                                            agcaaATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGACTTGGCAgatcg   >  1:1551906/1‑97 (MQ=255)
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AGATAAAGTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATGTTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAGATTGAAGCATCTGGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTCATCCAAGTTGATTTGGCTGAAGAA  >  NC_007793/141721‑141913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑