Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	123,798	T→C	Q5R (CAA→CGA)	SAUSA300_RS00565 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	123,798	0	T	C	100.0%	18.8 / NA	9	Q5R (CAA→CGA)	SAUSA300_RS00565	membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/3);  total (6/3)

GCACTAAAATCATTTAACGCAATCTTTATCATTGGAAATGCAGATCCCCACAATATGATAGTAAATAAATATGATAGAAAAGTAGTGTCTTGAAGTTTATTCATTAATATCATCACTCCTTTAATTATGTGTTTCTATATTAAAAAATATGATTTAAAATGA  >  NC_007793/123708‑123869                                                                                           |                                                                        gCACTAAAATCATTTAACGCAATCTTTATCATTGGAAATGCAGATCCCCACAATATGATAGTAAATAAATATGATAGAAAAGTAGTGTCTCGAAGTTTAt                                                                >  1:2597012/1‑100 (MQ=255) gCACTAAAATCATTTAACGCAATCTTTATCATTGGAAATGCAGATCCCCACAATATGATAGTAAATAAATATGATAGAAAAGTAGTGTCTCGAAGTTTAt                                                                >  1:5248469/1‑100 (MQ=255)                aaCGCAATCTTTATCATTGGAAATGCAGATCCCCACAATATGATAGTAAATAAATATGATAGAAAAGTAGTGTCTCGAAGTTTATTCATTAATAtcatca                                                 >  1:2151951/1‑100 (MQ=255)                                     aaTGCAGATCCCCACAATATGATAGTAAATAAATATGATAGAAAAGTAGTGTCTCGAAGTTTATTCATTAATATCATCACTCCTTTAATTATGTGTTTCt                            <  1:2192024/100‑1 (MQ=255)                                              gcccACAATATGATAGTAAATAAATATGATAGAAAAGTAGTGTCTCGAAGTTTATTCATTAATATCATCACTCCTTTAATTATGTGTTTCTATATTaaaa                   >  1:2830085/2‑100 (MQ=255)                                                ccACAATATGATAGTAAATAAATATGATAGAAAAGTAGTGTCTCGAAGTTTATTCATTAATATCATCACTCCTTTAATTATGTGTTTCTATATTaaaaaa                 >  1:4552464/1‑100 (MQ=255)                                                    agtatGATAGTAAATAAATATGATAGAAAAGTAGTGTCTCGAAGTTTATTCATTAATATCATCACTCCTTTAATTATGTGTTTCTATATTAAAAAATATg             <  1:5297512/98‑1 (MQ=255)                                                         gATAGTAAATAAATATGATAGAAAAGTAGTGTCTCGAAGTTTATTCATTAATATCATCACTCCTTTAATTATGTGTTTCTATATTAAAAAATATGATTTa        <  1:3602713/100‑1 (MQ=255)                                                               aaataaatATGATAGAAAAGTAGTGTCTCGAAGTTTATTCATTAATATCATCACTCCTTTAATTATGTGTTTCTATATTAAAAAATATGATTTAAAATGa  >  1:5523704/1‑100 (MQ=255)                                                                                           |                                                                        GCACTAAAATCATTTAACGCAATCTTTATCATTGGAAATGCAGATCCCCACAATATGATAGTAAATAAATATGATAGAAAAGTAGTGTCTTGAAGTTTATTCATTAATATCATCACTCCTTTAATTATGTGTTTCTATATTAAAAAATATGATTTAAAATGA  >  NC_007793/123708‑123869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑