Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	123,544	C→T	A90T (GCT→ACT)	SAUSA300_RS00565 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	123,544	0	C	T	100.0%	40.2 / NA	16	A90T (GCT→ACT)	SAUSA300_RS00565	membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/10);  total (6/10)

TAAAGCACTAGAAAAAATAGGCGTTGTAGAGACTAGAATTCCAGATATACCTGCACTAATCAAAGCTTCACCAAAATTTAAAGCTGTGTGATATATCACAAATCCACAAAATCCTAAAATAAAAATAACAGGGATATCTCTTAGTTCAGGGGTAGGCA  >  NC_007793/123479‑123636                                                                  |                                                                                             tccaGCACTAGAAAAAATAGGCGTTGTAGAGACTAGAATTCCAGATATACCTGCACTAATCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATcaca                                                            >  1:81630/4‑100 (MQ=255)  aaaGCACTAGAAAAAATAGGCGTTGTAGAGACTAGAATTCCAGATACACCTGCACTAATCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATCACaa                                                           >  1:2416324/1‑100 (MQ=255)                cttAGGCGTTGTAGAGACTAGAATTCCAGATATACCTGCACTAATCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATCACAAATCCACAAAATCCt                                             >  1:646517/3‑100 (MQ=255)                 gtAGGCGTTGTAGAGACTAGAATTCCAGATATACCTGCACTAATCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATCACAAATCCACAAAATCCta                                            >  1:5038542/2‑100 (MQ=255)                              agaCTAGAATTCCAGATATACCTGCACTAATCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATCACAAATCCACAAAATCCTAAAATAAAAATAAc                               >  1:4327877/1‑100 (MQ=255)                                  gtgctcttccgATCTATCTGCACTAATCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATCACAAATCCACAAAATCCTAAAATAAAAATAACAggg                           <  1:4663002/90‑1 (MQ=255)                                    gctcttccgATCTACCTGCACTAATCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATCACAAATCCACAAAATCCTAAAATAAAAATAACAGGGat                         <  1:1691117/92‑1 (MQ=255)                                                tgccTGCACTAATCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATCACAAATCCACAAAATCCTAAAATAAAAATAACAGGGATATCTCTTAGTTc             <  1:657623/98‑1 (MQ=255)                                                 aCCTGCACTAATCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATCACAAATCCACAAAATCCTAAAATAAAAATAACAGGGATATCTCTCAGTTCa            >  1:18650/1‑100 (MQ=255)                                                   cTGCACTAATCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATCACAAATCCACAAAATCCTAAAATAAAAATAACAGGGATATCTCTTAGTTCAgc          <  1:5184070/100‑2 (MQ=255)                                                    tGCACTAATCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATCACAAATCCACAAAATCCTAAAATAAAAATAACAGGGATATCTCTTAGTTCAggg         <  1:23184/100‑1 (MQ=255)                                                     gCACTAATCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATCACAAGTCCACAAAATACTAAAATAAAAATAACAGGGATATCTCTTAGTTCAgggg        <  1:1906385/100‑1 (MQ=255)                                                      cACTAATCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATCACAAATCCACAAAATCCTAAAATAAAAATAACAGGGATATCTCTTAGTTCAGGGGt       <  1:615739/100‑1 (MQ=255)                                                       aCTAATCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATCACAAATCCACAAAATCCTAAAATAAAAATAACAGGGATATCTCTTAGTTCAGGGGtc      <  1:1510519/100‑2 (MQ=255)                                                           aTCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATCACAAATCCACAAAATCCTAAAATAAAAATAACAGGGATATCTCTTAGTTCAGGGGTAGGCa  <  1:2764450/100‑1 (MQ=255)                                                           aTCAAAGTTTCACCAAAATTTAAAGCTGTGTGATATATCACAAATCCACAAAATCCTAAAATAAAAATAACAGGGATATCTCTTAGTTCAGGGGTAGGCa  <  1:3413721/100‑1 (MQ=255)                                                                  |                                                                                             TAAAGCACTAGAAAAAATAGGCGTTGTAGAGACTAGAATTCCAGATATACCTGCACTAATCAAAGCTTCACCAAAATTTAAAGCTGTGTGATATATCACAAATCCACAAAATCCTAAAATAAAAATAACAGGGATATCTCTTAGTTCAGGGGTAGGCA  >  NC_007793/123479‑123636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑