Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	46,072	A→G	Y483Y (TAT→TAC)	SAUSA300_RS00200 ←	recombinase RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	46,072	0	A	G	100.0%	89.1 / NA	34	Y483Y (TAT→TAC)	SAUSA300_RS00200	recombinase RecB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/32);  total (2/32)

GATTTCGTTATTAAGTTTGAGTGTGACATAGAATTGTTTTTTATGATTACCATCTTTTTTAATATCAATGCGATCAATCACTGATAGATACAATGCTTTGAGTCGAGATTTTTCTATGTGCTTAATATCATGAAAGATGTGTTGTAATAGTTTACTGATTTCTTTGGCATCAA  >  NC_007793/45985‑46157                                                                                        |                                                                                      gATTTCGTTATTAAGTTTGAGTGTGACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTg                                                                           <  1:4011933/100‑1 (MQ=255)     ttgTTATTAAGTTTGAGTGTGACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAgat                                                                       >  1:499406/3‑98 (MQ=255)      cGTTATTAAGTTTGAGTGTGACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCg                                                                      <  1:2218565/100‑1 (MQ=255)      cGTTATTAAGTTTGAGTGTGACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCg                                                                      <  1:4831558/100‑1 (MQ=255)      cGTTATTAAGTTTGAGTGTGACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCg                                                                      <  1:5712385/100‑1 (MQ=255)      cGTTATTAAGTTTGAGTGTGACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCg                                                                      <  1:2008559/100‑1 (MQ=255)                       gtgACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCt                                                     >  1:4739178/1‑100 (MQ=255)                        tgACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGGGATTTTTCCATGTGCtt                                                    <  1:695619/100‑1 (MQ=255)                        tgACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCtt                                                    <  1:5743295/100‑1 (MQ=255)                        tgACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCtt                                                    <  1:239737/100‑1 (MQ=255)                        tgACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCtt                                                    <  1:1522286/100‑1 (MQ=255)                        tgACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCtt                                                    <  1:466737/100‑1 (MQ=255)                        tgACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCtg                                                    <  1:808381/100‑2 (MQ=255)                        tgACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCta                                                    <  1:102945/100‑2 (MQ=255)                        tgACATAGAATTGTTTCTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCtt                                                    <  1:1973783/100‑1 (MQ=255)                        tcttccgatcttGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCtt                                                    <  1:1921013/90‑1 (MQ=255)                        tcttccgatcttGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCtt                                                    <  1:4277142/90‑1 (MQ=255)                        tcttccgatcttGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCtt                                                    <  1:3937289/90‑1 (MQ=255)                        tcttccgatcttGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCtt                                                    <  1:1410352/90‑1 (MQ=255)                        tcttccgatcttGTTCTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCtt                                                    <  1:2919369/90‑1 (MQ=255)                        tcttccgatcttGTCTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCtt                                                    <  1:3491555/90‑1 (MQ=255)                         gACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCttg                                                   <  1:3533550/100‑2 (MQ=255)                         gACATAGAATTGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCTTa                                                   <  1:1890527/100‑1 (MQ=255)                                 cttGTTTCTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCTTAATATcttt                                           <  1:1906206/99‑4 (MQ=255)                                   tGTTTTTTATGATTACCATCTTTTCTAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCTTAATATCTTGaa                                         <  1:897696/100‑1 (MQ=255)                                                          tctAATATTAATACGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCTTAATATCTTGAAAGATGTGTTGTAATAGTTTACTg                  <  1:5781827/98‑1 (MQ=255)                                                                    atacGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCTTAATATCTTGAAAGATGTGTTGTAATAGTTTACTGATTTGTTTgg        <  1:2085558/97‑1 (MQ=255)                                                                    atacGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCTCAATATCTTGAAAGATGTGTTGTAATAGTTTACTGATTTGTTTgg        <  1:5643970/97‑1 (MQ=255)                                                                     tccGATCTATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCTTAATATCTTGAAAGATGTGTTGTAATAGTTTACTGATTTGTTTGGc       <  1:279526/98‑1 (MQ=255)                                                                     tacGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCTTAATATCTTGAAAGATGTGTTGTAATAGTTTACTGATTTGTGTGGc       <  1:4798127/98‑1 (MQ=255)                                                                       cGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCTTAATATCTTGAAAGATGTGTTGTAATAGTTTACTGATTTGTTTGGCAt     <  1:2524489/100‑1 (MQ=255)                                                                       cGATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCTTAATATCTTGAAAGATGTGTTGTAATAGTTTACTGATTTGTTTGGCAt     <  1:2230666/100‑1 (MQ=255)                                                                        gATCAATCACTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCTTAATATCTTGAAAGATGTGTTGTAATAGTTTACTGATTTGTTTGGCAta    <  1:2877909/100‑2 (MQ=255)                                                                          tctatcaCTGATAGGTACAATGCTTTGAGTCGAGATTTTTCCATGTGCTTAATATCTTGAAAGATGTGTTGTAATAGTTTACTGATTTGTTTGGCATCaa  <  1:1816485/97‑1 (MQ=255)                                                                                        |                                                                                      GATTTCGTTATTAAGTTTGAGTGTGACATAGAATTGTTTTTTATGATTACCATCTTTTTTAATATCAATGCGATCAATCACTGATAGATACAATGCTTTGAGTCGAGATTTTTCTATGTGCTTAATATCATGAAAGATGTGTTGTAATAGTTTACTGATTTCTTTGGCATCAA  >  NC_007793/45985‑46157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑