Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	95,813	T→C	pseudogene (253/1194 nt)	SAUSA300_RS00450 →	pyridine nucleotide‑disulfide oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	95,813	0	T	C	100.0%	16.3 / NA	8	pseudogene (253/1194 nt)	SAUSA300_RS00450	pyridine nucleotide‑disulfide oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/4);  total (4/4)

TGGTGCAGGGGTATCTAGTTTGAAAAGTTCTCGTAAAGATATGGAAAGTGTTATACCTGAAGGTGCTAACTGGATAAAACAGGCTGTTTCAAGTTTTTAACCTGAAAATAATAGCGTTATTTTAGGAGATAATACAGTCGTTTATTATGATTTTTTAGTAGTAGCTCCAGGATTAC  >  NC_007793/95716‑95891                                                                                                  |                                                                               tggtGCAGGGGTATCTAGTTTGAAAAGTTCTCGTAAAGATATGGAAAGTGTTATACCTGAAGGTGCTAACTGGATAAAACAGGCTGTTTCAAGTTTTCaa                                                                              >  1:4386527/1‑100 (MQ=255)     gCAGGGGTATCTAGTTTGAAAAGTTCTCGTAAAGATATGGAAAGTGTTATACCTGAAGGTGCTAACTGGATAAAACAGGCTGTTTCAAGTTTTCAACCTg                                                                          >  1:1007763/1‑100 (MQ=255)                   tttGAAAAGTTCTCGTAAAGATATGGAAAGTGTTATACCTGAAGGTGCTAACTGGATAAAACAGGCTGTTTCAAGTTTTCAACCTGAAAATAATAGCGtt                                                            <  1:4431740/100‑1 (MQ=255)                                cGTAAAGATATGGAAAGTGTTATACCTGAAGGTGCTAACTGGATAAAACAGGCTGTTTCAAGTTTTCAACCTGAAAATAATAGCGTTATTTTAGGAGata                                               >  1:5534286/1‑100 (MQ=255)                                                               gTGCTAACTGGATAAAACAGGCTGTTTCAAGTTTTCAACCTGAAAATAATAGCGTTATTTTAGGAGATAATACAGTCGTTTATTATGATTTTTtagtagt                <  1:1021199/100‑1 (MQ=255)                                                               gTGCTAACTGGATAAAACAGGCTGTTTCAAGTTTTCAACCTGAAAATAATAGCGTTATTTTAGGAGATAATACAGTCGTTTATTATGATTTTTtagtagt                >  1:4815547/1‑100 (MQ=255)                                                                             aaaCAGGCTGTTTCAAGTTTTCAACCTGAAAATAATAGCGTTATTTTAGGAGATAATACAGTCGTTTATTATGATTTTTTAGTAGTAGCTCCAGGATTAc  <  1:1407029/100‑1 (MQ=255)                                                                             aaaCAGGCTGTTTCAAGTTTTCAACCTGAAAATAATAGCGTTATTTTAGGAGATAATACAGTCGTTTATTATGATTTTTTAGTAGTAGCTCCAGGATTAc  <  1:5219607/100‑1 (MQ=255)                                                                                                  |                                                                               TGGTGCAGGGGTATCTAGTTTGAAAAGTTCTCGTAAAGATATGGAAAGTGTTATACCTGAAGGTGCTAACTGGATAAAACAGGCTGTTTCAAGTTTTTAACCTGAAAATAATAGCGTTATTTTAGGAGATAATACAGTCGTTTATTATGATTTTTTAGTAGTAGCTCCAGGATTAC  >  NC_007793/95716‑95891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑