Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	76,402	C→T	S45L (TCA→TTA)	SAUSA300_RS00345 →	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	76,402	0	C	T	100.0%	137.2 / NA	61	S45L (TCA→TTA)	SAUSA300_RS00345	transposase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/46);  total (15/46)

TATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATA  >  NC_007793/76307‑76467                                                                                                |                                                                  tATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACg                                                               >  1:5581538/1‑100 (MQ=255)  aTCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACgg                                                              <  1:1306149/100‑1 (MQ=255)   tCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACggg                                                             <  1:754984/100‑2 (MQ=255)   tCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACggg                                                             <  1:2182359/100‑2 (MQ=255)     aCTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCt                                                           <  1:1126086/100‑1 (MQ=255)     aCTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCt                                                           <  1:4103319/100‑1 (MQ=255)     aCTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCt                                                           <  1:1878664/100‑1 (MQ=255)            acGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTg                                                    <  1:2343843/99‑1 (MQ=12)              gTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTggg                                                  <  1:5217637/100‑1 (MQ=12)                tagCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGtt                                                <  1:4264657/98‑1 (MQ=17)                tagCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGtt                                                <  1:86655/98‑1 (MQ=17)                tagCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGta                                                <  1:840966/98‑2 (MQ=12)                 agCTACTATCTAGGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTc                                               >  1:3518644/2‑100 (MQ=12)                 agCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGttg                                               <  1:4609906/99‑2 (MQ=17)                 agCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTc                                               <  1:4506349/99‑1 (MQ=17)                 agCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTc                                               <  1:980587/99‑1 (MQ=17)                 agCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTc                                               <  1:1342965/99‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGttac                                              <  1:2695507/100‑3 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:5358665/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:4336697/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:4807432/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:526531/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:5150317/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:4198667/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:4037333/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:5406061/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:5550981/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:3655329/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:872126/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:3249540/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:1831731/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:182729/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:2059131/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:875775/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:151247/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:2349892/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:706509/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:2742274/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:2896812/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:3108539/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:3488944/100‑1 (MQ=17)                  gCTACTATCTAAGATATGCATTGAGTTATCGTGATAAATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCa                                              <  1:2029203/100‑1 (MQ=12)                   ctactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCag                                             <  1:4680355/100‑2 (MQ=17)                   ctactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCaa                                             <  1:4898755/100‑1 (MQ=17)                     tctaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGa                                           >  1:5104443/2‑100 (MQ=17)                     actaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGGTCATCATTTAACGGTCTACCGTTGGGTTCAAGa                                           >  1:1460788/1‑100 (MQ=12)                        accTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGAata                                        >  1:4851021/3‑100 (MQ=17)                        aTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGAatg                                        <  1:1137052/100‑2 (MQ=17)                         tCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGAatat                                       <  1:3423106/100‑1 (MQ=17)                          cTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGAATATg                                      <  1:250179/100‑1 (MQ=17)                            aaGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGAATATGcc                                    >  1:679623/1‑100 (MQ=17)                            aaGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGAATATGcc                                    >  1:2590220/1‑100 (MQ=17)                            aaGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGAATATGcc                                    >  1:1210503/1‑100 (MQ=17)                                tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                >  1:4918694/1‑100 (MQ=17)                                tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                >  1:4711359/1‑100 (MQ=17)                                 atGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAt                               >  1:3902330/1‑100 (MQ=17)                                  tGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAtt                              >  1:1877101/1‑100 (MQ=14)                                                       cccTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaa         >  1:2471743/3‑100 (MQ=17)                                                           gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAgt     >  1:3830579/1‑99 (MQ=17)                                                           gAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAgt     >  1:2635098/1‑99 (MQ=17)                                                              atatTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGTAtc  <  1:1670251/100‑2 (MQ=12)                                                                                                |                                                                  TATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATA  >  NC_007793/76307‑76467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑