Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	76,323	G→A	G19S (GGC→AGC)	SAUSA300_RS00345 →	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	76,323	0	G	A	100.0%	46.9 / NA	22	G19S (GGC→AGC)	SAUSA300_RS00345	transposase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/21);  total (1/21)

ATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGG  >  NC_007793/76285‑76419                                       |                                                                                                 ataAACAATTTAACAAGGATGTTAGCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtgg                                     <  1:3915039/100‑1 (MQ=255)  taAACAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtgca                                    <  1:4849248/100‑3 (MQ=255)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                  <  1:1325757/100‑1 (MQ=12)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                  <  1:1671390/100‑1 (MQ=12)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                  <  1:1679305/100‑1 (MQ=12)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                  <  1:5287798/100‑1 (MQ=12)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                  <  1:2311167/100‑1 (MQ=12)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                  <  1:5274040/100‑1 (MQ=12)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                  <  1:2897256/100‑1 (MQ=12)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                  <  1:4556660/100‑1 (MQ=12)     aCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                                 <  1:605609/100‑1 (MQ=12)     aCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                                 <  1:4075830/100‑1 (MQ=12)      cAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGCGATATATCTGAAATATTAAGGGAACGTGGTGTaa                                <  1:2159531/100‑1 (MQ=255)                       tATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACg               >  1:5581538/1‑100 (MQ=255)                        aTCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACgg              <  1:1306149/100‑1 (MQ=255)                         tCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACggg             <  1:2182359/100‑2 (MQ=255)                         tCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACggg             <  1:754984/100‑2 (MQ=255)                           aCTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCt           <  1:4103319/100‑1 (MQ=255)                           aCTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCt           <  1:1878664/100‑1 (MQ=255)                           aCTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCt           <  1:1126086/100‑1 (MQ=255)                                  acGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTg    <  1:2343843/99‑1 (MQ=12)                                    gTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTggg  <  1:5217637/100‑1 (MQ=12)                                       |                                                                                                 ATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGG  >  NC_007793/76285‑76419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑