Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	76,318	C→A	A17D (GCC→GAC)	SAUSA300_RS00345 →	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	76,318	0	C	A	100.0%	42.7 / NA	20	A17D (GCC→GAC)	SAUSA300_RS00345	transposase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (1/19);  total (1/19)

ATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCT  >  NC_007793/76285‑76410                                  |                                                                                             ataAACAATTTAACAAGGATGTTAGCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtgg                            <  1:3915039/100‑1 (MQ=255)  taAACAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtgca                           <  1:4849248/100‑3 (MQ=255)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                         <  1:1325757/100‑1 (MQ=12)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                         <  1:1671390/100‑1 (MQ=12)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                         <  1:1679305/100‑1 (MQ=12)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                         <  1:5287798/100‑1 (MQ=12)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                         <  1:2311167/100‑1 (MQ=12)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                         <  1:2897256/100‑1 (MQ=12)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                         <  1:5274040/100‑1 (MQ=12)    aaCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                         <  1:4556660/100‑1 (MQ=12)     aCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                        <  1:605609/100‑1 (MQ=12)     aCAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                        <  1:4075830/100‑1 (MQ=12)      cAATTTAACAAGGATGTTATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGCGATATATCTGAAATATTAAGGGAACGTGGTGTaa                       <  1:2159531/100‑1 (MQ=255)                       tATCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACg      >  1:5581538/1‑100 (MQ=255)                        aTCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACgg     <  1:1306149/100‑1 (MQ=255)                         tCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACggg    <  1:2182359/100‑2 (MQ=255)                         tCACTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACggg    <  1:754984/100‑2 (MQ=255)                           aCTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCt  <  1:4103319/100‑1 (MQ=255)                           aCTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCt  <  1:1878664/100‑1 (MQ=255)                           aCTGTAGACGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCt  <  1:1126086/100‑1 (MQ=255)                                  |                                                                                             ATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCT  >  NC_007793/76285‑76410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑